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UniProtKB/Swiss-Prot entry P32891


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DLD1_YEAST
Primary accession number P32891
Secondary accession number Q12360
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on November 1, 1997 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 77)
Name and origin of the protein
Protein name D-lactate dehydrogenase [cytochrome] 1, mitochondrial [Precursor]
Synonyms EC 1.1.2.4
D-lactate ferricytochrome C oxidoreductase
D-LCR
Gene name
Name: DLD1
Synonyms: DLD
OrderedLocusNames: YDL174C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1007/BF00291989; PubMed=8492799 [NCBI, ExPASy, EBI, Israel, Japan]
Lodi T., Ferrero I.;
"Isolation of the DLD gene of Saccharomyces cerevisiae encoding the mitochondrial enzyme D-lactate ferricytochrome c oxidoreductase.";
Mol. Gen. Genet. 238:315-324(1993).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=9169867 [NCBI, ExPASy, EBI, Israel, Japan]
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
Nature 387:75-78(1997).
[3]
PROTEIN SEQUENCE OF 576-581, AND SUBCELLULAR LOCATION.
STRAIN=ATCC 201238 / W303-1B;
DOI=10.1021/bi010277r; PubMed=11502169 [NCBI, ExPASy, EBI, Israel, Japan]
Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N., Manon S., Schmitter J.-M.;
"Yeast mitochondrial dehydrogenases are associated in a supramolecular complex.";
Biochemistry 40:9758-9769(2001).
[4]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X66052; CAA46852.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z67750; CAA91571.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z74222; CAA98748.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S61038; S61038.
RefSeq NP_010107.1; -.
3D structure databases
ModBase P32891.
Protein-protein interaction databases
IntAct P32891; -.
Organism-specific databases
CYGD YDL174c; -.
SGD S000002333; DLD1.
Yeast-GFP YDL174C.
Gene expression databases
ArrayExpress P32891; -.
GermOnline YDL174C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005743; Cellular component: mitochondrial inner membrane (inferred from direct assay from SGD).
GO:0004458; Molecular function: D-lactate dehydrogenase (cytochrome) activity (inferred from mutant phenotype from SGD).
GO:0009060; Biological process: aerobic respiration (inferred from mutant phenotype from SGD).
GO:0005975; Biological process: carbohydrate metabolic process (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR016167; FAD-bd_2_sub1.
IPR016168; FAD-linked_Oxase_FAD-bd_sub2.
IPR004113; FAD-linked_oxidase_C.
IPR006094; Oxid_FAD_bind_N.
Graphical view of domain structure.
Gene3D G3DSA:3.30.43.10; FAD-binding_2_sub1; 1.
G3DSA:3.30.465.20; FAD-linked_oxidase_FAD-bd_sub2; 1.
Pfam PF02913; FAD-oxidase_C; 1.
PF01565; FAD_binding_4; 1.
Pfam graphical view of domain structure.
PROSITE PS51387; FAD_PCMH; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P32891.
Proteomic databases
PeptideAtlas P32891; -.
Genome annotation databases
Ensembl YDL174C; Saccharomyces cerevisiae. [Contig view]
GeneID 851380; -.
GenomeReviews Z71256_GR; YDL174C.
KEGG sce:YDL174C; -.
NMPDR fig|4932.3.peg.841; -.
Phylogenomic databases
HOGENOM P32891; -.
Other
LinkHub P32891; -.
ProtoNet P32891.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; FAD; Flavoprotein; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Transit peptide; Zinc.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1     ?        Mitochondrion. 
CHAIN   ?   587        D-lactate dehydrogenase [cytochrome] 1, mitochondrial. PRO_0000020428
DOMAIN   146   327  182     FAD-binding PCMH-type. 
CONFLICT   439   439        A -> RR (in Ref. 1; CAA46852). 
CONFLICT   572   587        DKIFKTDPNEPANDYR -> GQNL (in Ref. 1; CAA46852). 
Sequence information
Length: 587 AA [This is the length of the unprocessed precursor] Molecular weight: 65293 Da [This is the MW of the unprocessed precursor] CRC64: 07183BEAEEB2EB19 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLWKRTCTRL IKPIAQPRGR LVRRSCYRYA STGTGSTDSS SQWLKYSVIA SSATLFGYLF 

        70         80         90        100        110        120 
AKNLYSRETK EDLIEKLEMV KKIDPVNSTL KLSSLDSPDY LHDPVKIDKV VEDLKQVLGN 

       130        140        150        160        170        180 
KPENYSDAKS DLDAHSDTYF NTHHPSPEQR PRIILFPHTT EEVSKILKIC HDNNMPVVPF 

       190        200        210        220        230        240 
SGGTSLEGHF LPTRIGDTIT VDLSKFMNNV VKFDKLDLDI TVQAGLPWED LNDYLSDHGL 

       250        260        270        280        290        300 
MFGCDPGPGA QIGGCIANSC SGTNAYRYGT MKENIINMTI VLPDGTIVKT KKRPRKSSAG 

       310        320        330        340        350        360 
YNLNGLFVGS EGTLGIVTEA TVKCHVKPKA ETVAVVSFDT IKDAAACASN LTQSGIHLNA 

       370        380        390        400        410        420 
MELLDENMMK LINASESTDR CDWVEKPTMF FKIGGRSPNI VNALVDEVKA VAQLNHCNSF 

       430        440        450        460        470        480 
QFAKDDDEKL ELWEARKVAL WSVLDADKSK DKSAKIWTTD VAVPVSQFDK VIHETKKDMQ 

       490        500        510        520        530        540 
ASKLINAIVG HAGDGNFHAF IVYRTPEEHE TCSQLVDRMV KRALNAEGTC TGEHGVGIGK 

       550        560        570        580 
REYLLEELGE APVDLMRKIK LAIDPKRIMN PDKIFKTDPN EPANDYR 

P32891 in FASTA format

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