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UniProtKB/Swiss-Prot entry P33057


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MCEL_VARV
Primary accession number P33057
Secondary accession number Q9QNI8
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on October 1, 1993 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 49)
Name and origin of the protein
Protein name mRNA-capping enzyme large subunit
Synonyms None
Includes Polynucleotide 5'-triphosphatase
     (EC 3.1.3.33)
     (mRNA 5'-triphosphatase)
     (TPase)
mRNA guanylyltransferase
     (EC 2.7.7.50)
     (GTP--RNA guanylyltransferase)
     (GTase)
Gene name
ORFNames: D1R, F1R
From
Variola virus [TaxID: 10255] 
Taxonomy Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
Virus host Homo sapiens (Human) [TaxID: 9606]
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=India-1967 / Isolate Ind3;
DOI=10.1016/0168-1702(93)90110-9; PubMed=8383392 [NCBI, ExPASy, EBI, Israel, Japan]
Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S., Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V., Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G., Sandakhchiev L.S.;
"Nucleotide sequence analysis of variola virus HindIII M, L, I genome fragments.";
Virus Res. 27:25-35(1993).
[2]
COMPLETE GENOME.
STRAIN=India-1967 / Isolate Ind3;
DOI=10.1016/0014-5793(93)80041-R; PubMed=8384129 [NCBI, ExPASy, EBI, Israel, Japan]
Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
"Genes of variola and vaccinia viruses necessary to overcome the host protective mechanisms.";
FEBS Lett. 319:80-83(1993).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Bangladesh-1975;
DOI=10.1038/366748a0; PubMed=8264798 [NCBI, ExPASy, EBI, Israel, Japan]
Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C., Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A., Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.;
"Potential virulence determinants in terminal regions of variola smallpox virus genome.";
Nature 366:748-751(1993).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Garcia-1966;
DOI=10.1006/viro.1999.0086; PubMed=10639322 [NCBI, ExPASy, EBI, Israel, Japan]
Shchelkunov S.N., Totmenin A.V., Loparev V.N., Safronov P.F., Gutorov V.V., Chizhikov V.E., Knight J.C., Parsons J.M., Massung R.F., Esposito J.J.;
"Alastrim smallpox variola minor virus genome DNA sequences.";
Virology 266:361-386(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X67119; CAA47590.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X69198; CAA49032.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
L22579; AAA60839.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Y16780; CAB54691.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A72162; A72162.
G36846; G36846.
T28529; T28529.
RefSeq NP_042135.1; -.
3D structure databases
ModBase P33057.
Ontologies
GO
GO:0004484; Molecular function: mRNA guanylyltransferase activity (inferred from electronic annotation from EC).
GO:0004651; Molecular function: polynucleotide 5'-phosphatase activity (inferred from electronic annotation from EC).
GO:0006370; Biological process: mRNA capping (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR004971; Pox_MCEL.
Graphical view of domain structure.
Pfam PF03291; Pox_MCEL; 1.
Pfam graphical view of domain structure.
ProtoNet P33057.
Genome annotation databases
GeneID 1486417; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Hydrolase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotidyltransferase; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   844  844     mRNA-capping enzyme large subunit. PRO_0000210125
ACT_SITE   260   260        N6-GMP-lysine intermediate (Potential). 
VARIANT   118   118  1     A -> E (in strain: Bangladesh-1975 and Garcia-1966). 
VARIANT   552   552  1     E -> D (in strain: Garcia-1966). 
VARIANT   600   600  1     E -> G (in strain: Bangladesh-1975 and Garcia-1966). 
VARIANT   741   741  1     V -> A (in strain: Garcia-1966). 
VARIANT   831   831  1     D -> N (in strain: Garcia-1966). 
Sequence information
Length: 844 AA [This is the length of the unprocessed precursor] Molecular weight: 96729 Da [This is the MW of the unprocessed precursor] CRC64: CAA2474D798115D1 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDANVVSSST IATYIDALAK NASELEQGST AYEINNELEL VFIKPPLITL TNVVNISTIQ 

        70         80         90        100        110        120 
ESFIRFTVTN KEGVKIRTKI PLSKVHGLDV KNVQLVDAID NIVWEKKSLV TENRLHKACL 

       130        140        150        160        170        180 
LRLSTEERHI FLDYKKYGSS IRLELVNLIQ AKTKNFTIDF KLKYFLGSGA QSKSSLLHAI 

       190        200        210        220        230        240 
NHPKSRPNTS LEIEFTPRDN ETVPYDELIK ELTTFSRHIF MASPENVILS PPINAPIKTF 

       250        260        270        280        290        300 
MLPKQDIVGM DLENLYAVTK TDGIPITIRV TSKGLYCYFT HLGYIIRYPV KRIIDSEVVV 

       310        320        330        340        350        360 
FGEAVKDKNW TVYLIKLIEP VNAISDRLEE SKYVESKLVD ICDRIVFKSK KYEGPFTTTS 

       370        380        390        400        410        420 
EVVDMLSTYL PKQPEGVILF YSKGPKSNID FKIKKENTID QTVNVVFRYM SSEPIIFGES 

       430        440        450        460        470        480 
SIFIEYKKFT NDKGFPKEYG SGKIVLYNGV NYLNNIYCLE YINTHNEVGI KSVVVPIKFI 

       490        500        510        520        530        540 
AEFLVNGEIL KPRIDKTMKY INSEDYYGNQ HNVIVEHLRD QSIKIGDVFN EDKLSDVGHQ 

       550        560        570        580        590        600 
YANNDKFRLN PEVSYFTNKR TRGPLGILSN YVKTLLISMY CSKTFLDDSN KRKVLAIDFE 

       610        620        630        640        650        660 
NGADLEKYFY GEIALLVATD PDADAIARGN ERYNKLNSGI KTKYYKFDYI QETIRSNTFV 

       670        680        690        700        710        720 
SSVREVFYFG KFNIIDWQFA IHYSFHPRHY ATIMNNLSEL TASGGKVLIT TMDGDKLSKL 

       730        740        750        760        770        780 
TDKKTFIIHK NLPSSENYMS VEKIADDRIV VYNPSTMSTP MTEYIIKKND IVRVFNEYGF 

       790        800        810        820        830        840 
VLVDNVDFAT IIERSKKFIN GASTMEDRPS TRNFFELNRG AIKCEGLDVE DLLSYYVVYV 


FSKR 

P33057 in FASTA format

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