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UniProtKB/Swiss-Prot entry P33203

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PRP40_YEAST
Primary accession number P33203
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1994
Sequence was last modified on February 1, 1994 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 83)
Name and origin of the protein
Protein name Pre-mRNA-processing protein PRP40
Synonyms None
Gene name
Name: PRP40
OrderedLocusNames: YKL012W
ORFNames: YKL165
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1002/yea.320081109; PubMed=1481574 [NCBI, ExPASy, EBI, Israel, Japan]
Pascolo S., Ghazvini M., Boyer J., Colleaux L., Thierry A., Dujon B.;
"The sequence of a 9.3 kb segment located on the left arm of the yeast chromosome XI reveals five open reading frames including the CCE1 gene and putative products related to MYO2 and to the ribosomal protein L10.";
Yeast 8:987-995(1992).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
DOI=10.1038/369371a0; PubMed=8196765 [NCBI, ExPASy, EBI, Israel, Japan]
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.;
"Complete DNA sequence of yeast chromosome XI.";
Nature 369:371-378(1994).
[3]
 PARTIAL PROTEIN SEQUENCE.
DOI=10.1073/pnas.94.2.385; PubMed=9012791 [NCBI, ExPASy, EBI, Israel, Japan]
Neubauer G., Gottschalk A., Fabrizio P., Seraphin B., Luehrmann R., Mann M.;
"Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry.";
Proc. Natl. Acad. Sci. U.S.A. 94:385-390(1997).
[4]
 IDENTIFICATION, AND MUTAGENESIS OF SER-240.
PubMed=8622699 [NCBI, ExPASy, EBI, Israel, Japan]
Kao H.-Y., Siliciano P.G.;
"Identification of Prp40, a novel essential yeast splicing factor associated with the U1 small nuclear ribonucleoprotein particle.";
Mol. Cell. Biol. 16:960-967(1996).
[5]
 FUNCTION, AND INTERACTION WITH MSL5; MUD2 AND PRP8.
DOI=10.1016/S0092-8674(00)80221-4; PubMed=9150140 [NCBI, ExPASy, EBI, Israel, Japan]
Abovich N., Rosbash M.;
"Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals.";
Cell 89:403-412(1997).
[6]
 FUNCTION, AND INTERACTION WITH RPB1.
DOI=10.1074/jbc.M004118200; PubMed=10978320 [NCBI, ExPASy, EBI, Israel, Japan]
Morris D.P., Greenleaf A.L.;
"The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II.";
J. Biol. Chem. 275:39935-39943(2000).
[7]
 FUNCTION, INTERACTION WITH CRM1, SUBCELLULAR LOCATION, AND MUTAGENESIS OF LEU-274; LEU-277; LEU-281; LEU-340; LEU-344 AND LEU-347.
DOI=10.1534/genetics.166.1.53; PubMed=15020406 [NCBI, ExPASy, EBI, Israel, Japan]
Murphy M.W., Olson B.L., Siliciano P.G.;
"The yeast splicing factor Prp40p contains functional leucine-rich nuclear export signals that are essential for splicing.";
Genetics 166:53-65(2004).
[8]
 PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-576 AND TYR-583, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
[9]
 STRUCTURE BY NMR OF 1-75.
DOI=10.1016/S0022-2836(02)01145-2; PubMed=12460579 [NCBI, ExPASy, EBI, Israel, Japan]
Wiesner S., Stier G., Sattler M., Macias M.J.;
"Solution structure and ligand recognition of the WW domain pair of the yeast splicing factor Prp40.";
J. Mol. Biol. 324:807-822(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
S53418; AAB24902.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z28012; CAA81847.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S30014; S30014.
RefSeq NP_012913.1; -.
3D structure databases
PDB
1O6W; NMR; -; A=1-75.[ExPASy / RCSB / EBI]
2B7E; NMR; -; A=134-189.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1O6W; -.
2B7E; -.
ModBase P33203.
Protein-protein interaction databases
DIP DIP:1620N; -.
IntAct P33203; -.
Organism-specific databases
CYGD YKL012w; -.
SGD S000001495; PRP40.
Yeast-GFP YKL012W.
Gene expression databases
ArrayExpress P33203; -.
GermOnline YKL012W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005685; Cellular component: snRNP U1 (inferred from direct assay from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0003723; Molecular function: RNA binding (inferred from direct assay from SGD).
GO:0000398; Biological process: nuclear mRNA splicing, via spliceosome (inferred from direct assay from SGD).
QuickGo view.
Family and domain databases
InterPro IPR002713; FF.
IPR001202; WW_Rsp5_WWP.
Graphical view of domain structure.
Gene3D G3DSA:1.10.10.440; FF; 1.
Pfam PF01846; FF; 4.
PF00397; WW; 2.
Pfam graphical view of domain structure.
SMART SM00441; FF; 4.
SM00456; WW; 2.
SMART graphical view of domain structure.
PROSITE PS01159; WW_DOMAIN_1; 2.
PS50020; WW_DOMAIN_2; 2.
PROSITE graphical view of domain structure (profiles).
ProtoNet P33203.
Genome annotation databases
Ensembl YKL012W; Saccharomyces cerevisiae. [Contig view]
GeneID 853857; -.
GenomeReviews Y13137_GR; YKL012W.
KEGG sce:YKL012W; -.
NMPDR fig|4932.3.peg.3899; -.
Phylogenomic databases
HOGENOM P33203; -.
Other
LinkHub P33203; -.
NextBio 975100; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Repeat; Ribonucleoprotein.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   583  583     Pre-mRNA-processing protein PRP40. PRO_0000076084
DOMAIN   1    31  31     WW 1. 
DOMAIN   42    72  31     WW 2. 
DOMAIN   134   185  52     FF 1. 
DOMAIN   203   254  52     FF 2. 
DOMAIN   357   410  54     FF 3. 
DOMAIN   494   549  56     FF 4. 
MOD_RES   576   576        Phosphothreonine. 
MOD_RES   583   583        Phosphotyrosine. 
MUTAGEN   240   240        S->P: In PRP40-1 suppressor; affects SAR1 mRNA accumulation in U1-U4 mutant at 18 degrees Celsius. 
MUTAGEN   274   274        L->A: Temperature sensitive growth at 36 degrees Celsius. 
MUTAGEN   277   277        L->A: Temperature sensitive growth at 36 degrees Celsius. 
MUTAGEN   281   281        L->A: Temperature sensitive growth at 36 degrees Celsius. 
MUTAGEN   340   340        L->A: Wild-type growth at 36 degrees Celsius. 
MUTAGEN   344   344        L->A: Wild-type growth at 36 degrees Celsius. 
MUTAGEN   347   347        L->I: Wild-type growth at 36 degrees Celsius. 
STRAND   4     8  5      
STRAND   14    18  5      
TURN   19    22  4      
STRAND   23    27  5      
HELIX   30    43  14      
STRAND   46    49  4      
STRAND   55    59  5      
TURN   60    63  4      
STRAND   64    68  5      
HELIX   134   145  12      
HELIX   154   164  11      
HELIX   167   170  4      
HELIX   175   187  13      
Sequence information
Length: 583 AA [This is the length of the unprocessed precursor] Molecular weight: 69065 Da [This is the MW of the unprocessed precursor] CRC64: BA1C1C91D532524C [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSIWKEAKDA SGRIYYYNTL TKKSTWEKPK ELISQEELLL RENGWKAAKT ADGKVYYYNP 

        70         80         90        100        110        120 
TTRETSWTIP AFEKKVEPIA EQKHDTVSHA QVNGNRIALT AGEKQEPGRT INEEESQYAN 

       130        140        150        160        170        180 
NSKLLNVRRR TKEEAEKEFI TMLKENQVDS TWSFSRIISE LGTRDPRYWM VDDDPLWKKE 

       190        200        210        220        230        240 
MFEKYLSNRS ADQLLKEHNE TSKFKEAFQK MLQNNSHIKY YTRWPTAKRL IADEPIYKHS 

       250        260        270        280        290        300 
VVNEKTKRQT FQDYIDTLID TQKESKKKLK TQALKELREY LNGIITTSSS ETFITWQQLL 

       310        320        330        340        350        360 
NHYVFDKSKR YMANRHFKVL THEDVLNEYL KIVNTIENDL QNKLNELRLR NYTRDRIARD 

       370        380        390        400        410        420 
NFKSLLREVP IKIKANTRWS DIYPHIKSDP RFLHMLGRNG SSCLDLFLDF VDEQRMYIFA 

       430        440        450        460        470        480 
QRSIAQQTLI DQNFEWNDAD SDEITKQNIE KVLENDRKFD KVDKEDISLI VDGLIKQRNE 

       490        500        510        520        530        540 
KIQQKLQNER RILEQKKHYF WLLLQRTYTK TGKPKPSTWD LASKELGESL EYKALGDEDN 

       550        560        570        580 
IRRQIFEDFK PESSAPTAES ATANLTLTAS KKRHLTPAVE LDY
P33203 in FASTA format

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