ID   DHE2_YEAST              Reviewed;        1092 AA.
AC   P33327;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-NOV-2008, entry version 72.
DE   RecName: Full=NAD-specific glutamate dehydrogenase;
DE            Short=NAD-GDH;
DE            EC=1.4.1.2;
GN   Name=GDH2; OrderedLocusNames=YDL215C; ORFNames=D0892;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=EBY23;
RX   MEDLINE=94039072; PubMed=7901008;
RA   Boles E., Lehnert W., Zimmermann F.K.;
RT   "The role of the NAD-dependent glutamate dehydrogenase in restoring
RT   growth on glucose of a Saccharomyces cerevisiae phosphoglucose
RT   isomerase mutant.";
RL   Eur. J. Biochem. 217:469-477(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   MEDLINE=97313263; PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N.,
RA   Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M.,
RA   Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L.,
RA   Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M.,
RA   Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S.,
RA   Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M.,
RA   Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S.,
RA   Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K.,
RA   Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D.,
RA   Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C.,
RA   Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T.,
RA   Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W.,
RA   Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K.,
RA   Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S.,
RA   Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A.,
RA   Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S.,
RA   Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M.,
RA   Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y.,
RA   Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M.,
RA   Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E.,
RA   Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R.,
RA   Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-79.
RX   MEDLINE=92049354; PubMed=1682801;
RA   Miller S.M., Magasanik B.;
RT   "Role of the complex upstream region of the GDH2 gene in nitrogen
RT   regulation of the NAD-linked glutamate dehydrogenase in Saccharomyces
RT   cerevisiae.";
RL   Mol. Cell. Biol. 11:6229-6247(1991).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 848-1092.
RA   Rasmussen S.W.;
RL   Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-482, AND MASS
RP   SPECTROMETRY.
RX   PubMed=15665377; DOI=10.1074/mcp.M400219-MCP200;
RA   Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J.,
RA   Mann M., Jensen O.N.;
RT   "Quantitative phosphoproteomics applied to the yeast pheromone
RT   signaling pathway.";
RL   Mol. Cell. Proteomics 4:310-327(2005).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25; SER-28 AND SER-242,
RP   AND MASS SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- CATALYTIC ACTIVITY: L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate
CC       + NH(3) + NADH.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- MISCELLANEOUS: Present with 7400 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; S66436; AAB20327.1; -; Genomic_DNA.
DR   EMBL; X99000; CAA67475.1; -; Genomic_DNA.
DR   EMBL; Z74263; CAA98793.1; -; Genomic_DNA.
DR   EMBL; X72015; CAA50894.1; -; Genomic_DNA.
DR   PIR; S37676; S37676.
DR   RefSeq; NP_010066.1; -.
DR   DIP; DIP:1689N; -.
DR   IntAct; P33327; -.
DR   PeptideAtlas; P33327; -.
DR   Ensembl; YDL215C; Saccharomyces cerevisiae.
DR   GeneID; 851311; -.
DR   GenomeReviews; Z71256_GR; YDL215C.
DR   KEGG; sce:YDL215C; -.
DR   NMPDR; fig|4932.3.peg.799; -.
DR   CYGD; YDL215c; -.
DR   SGD; S000002374; GDH2.
DR   HOGENOM; P33327; -.
DR   LinkHub; P33327; -.
DR   NextBio; 968334; -.
DR   ArrayExpress; P33327; -.
DR   GermOnline; YDL215C; Saccharomyces cerevisiae.
DR   GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004352; F:glutamate dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0019551; P:glutamate catabolic process to 2-oxoglutarate; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:InterPro.
DR   InterPro; IPR006095; Glu/Leu/Phe/Val_DHase.
DR   InterPro; IPR006096; Glu/Leu/Phe/Val_DHase_C.
DR   InterPro; IPR016210; Glu_DHase_NAD-dep.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00208; ELFV_dehydrog; 1.
DR   PIRSF; PIRSF000184; GDH_NAD; 1.
DR   PROSITE; PS00074; GLFV_DEHYDROGENASE; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; NAD; Oxidoreductase; Phosphoprotein.
FT   CHAIN         1   1092       NAD-specific glutamate dehydrogenase.
FT                                /FTId=PRO_0000182733.
FT   ACT_SITE    626    626       By similarity.
FT   MOD_RES      25     25       Phosphoserine.
FT   MOD_RES      28     28       Phosphoserine.
FT   MOD_RES     242    242       Phosphoserine.
FT   MOD_RES     482    482       Phosphoserine.
SQ   SEQUENCE   1092 AA;  124332 MW;  DF8358D831E4545F CRC64;
     MLFDNKNRGA LNSLNTPDIA SLSISSMSDY HVFDFPGKDL QREEVIDLLD QQGFIPDDLI
     EQEVDWFYNS LGIDDLFFSR ESPQLISNII HSLYASKLDF FAKSKFNGIQ PRLFSIKNKI
     ITNDNHAIFM ESNTGVSISD SQQKNFKFAS DAVGNDTLEH GKDTIKKNRI EMDDSCPPYE
     LDSEIDDLFL DNKSQKNCRL VSFWAPESEL KLTFVYESVY PNDDPAGVDI SSQDLLKGDI
     ESISDKTMYK VSSNENKKLY GLLLKLVKER EGPVIKTTRS VENKDEIRLL VAYKRFTTKR
     YYSALNSLFH YYKLKPSKFY LESFNVKDDD IIIFSVYLNE NQQLEDVLLH DVEAALKQVE
     REASLLYAIP NNSFHEVYQR RQFSPKEAIY AHIGAIFINH FVNRLGSDYQ NLLSQITIKR
     NDTTLLEIVE NLKRKLRNET LTQQTIINIM SKHYTIISKL YKNFAQIHYY HNSTKDMEKT
     LSFQRLEKVE PFKNDQEFEA YLNKFIPNDS PDLLILKTLN IFNKSILKTN FFITRKVAIS
     FRLDPSLVMT KFEYPETPYG IFFVVGNTFK GFHIRFRDIA RGGIRIVCSR NQDIYDLNSK
     NVIDENYQLA STQQRKNKDI PEGGSKGVIL LNPGLVEHDQ TFVAFSQYVD AMIDILINDP
     LKENYVNLLP KEEILFFGPD EGTAGFVDWA TNHARVRNCP WWKSFLTGKS PSLGGIPHDE
     YGMTSLGVRA YVNKIYETLN LTNSTVYKFQ TGGPDGDLGS NEILLSSPNE CYLAILDGSG
     VLCDPKGLDK DELCRLAHER KMISDFDTSK LSNNGFFVSV DAMDIMLPNG TIVANGTTFR
     NTFHTQIFKF VDHVDIFVPC GGRPNSITLN NLHYFVDEKT GKCKIPYIVE GANLFITQPA
     KNALEEHGCI LFKDASANKG GVTSSSMEVL ASLALNDNDF VHKFIGDVSG ERSALYKSYV
     VEVQSRIQKN AELEFGQLWN LNQLNGTHIS EISNQLSFTI NKLNDDLVAS QELWLNDLKL
     RNYLLLDKII PKILIDVAGP QSVLENIPES YLKVLLSSYL SSTFVYQNGI DVNIGKFLEF
     IGGLKREAEA SA
//