[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=1294332 [NCBI, ExPASy, EBI, Israel, Japan] Yagi K., Seto T., Terakado M., Umeda F., Doi T., Imanishi T., Miura Y.; "Nucleotide sequences of membrane-bound hydrogenase gene in Alcaligenes hydrogenophilus."; Chem. Pharm. Bull. 40:3292-3296(1992).

Comments

FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
CATALYTIC ACTIVITY: H₂ + A = AH₂.
COFACTOR: Binds 1 nickel ion per subunit (By similarity).
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

S56898; AAB25780.1; ALT_SEQ; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

JH0776; JH0776.

3D structure databases

HSSP

P21852; 1UBL. [HSSP ENTRY / PDB]

ModBase

P33374.

Family and domain databases

InterPro

IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.

Pfam

PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.

PROSITE

PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Membrane; Metal-binding; Nickel; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 621`	`621`	`Uptake hydrogenase large subunit.`	`PRO_0000199707`
`METAL`	`75 75`		`Nickel (Potential).`
`METAL`	`78 78`		`Nickel (Potential).`
`METAL`	`600 600`		`Nickel (Potential).`
`METAL`	`603 603`		`Nickel (Potential).`

Sequence information

Length: 621 AA [This is the length of the unprocessed precursor]

Molecular weight: 68925 Da [This is the MW of the unprocessed precursor]

CRC64: 906AC4414285D4A3 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MATYETQGFK LNDSGRRIIV DPVTRIEGHM RCEVNLDANN VIRNAVSTGT MWRGLEVILK 

        70         80         90        100        110        120 
RADPADAWAF VERICRVCTG CHALASVRAV EDALGIKIPK NAHLIREMMA KTLQVHDHVV 

       130        140        150        160        170        180 
HFYHLHALDW VDVVSALNAD PKRTSALQQT VSPAHPLSSP GYFRDVQIRL KKFVESGQLG 

       190        200        210        220        230        240 
PFMNGYWGNP AYKLPPEANL MAVTHYLEAL DLQKEWVKIH TIFGGKNPHP NYLVGGMPCV 

       250        260        270        280        290        300 
DSNLDGSGAA GAPLNMERLN FVRARIEEAI EFVKNVYLPD VLAIGTIYKD AGWLYGGGLS 

       310        320        330        340        350        360 
ALNVMDYGTY PRVNYDPTTD QLPGGAILNG NWDEIFPVDP RDPEQVQEFV AHSWYKYADE 

       370        380        390        400        410        420 
TKGLHPWDGV TEPNFVLGPK AVGTPTDIKQ LDEDAKYSWI KVAALAGHAM EVGPLVALHP 

       430        440        450        460        470        480 
RIRARAEDPK SYRAHYLREQ VENSARAINT GIPQALGLKQ TDYTVKQLLP TTIGRTLARA 

       490        500        510        520        530        540 
LEAQYCGNMM LDDWHEMMAN IKAGDLTTAN VDKWEPSAWP KEAKGVGHVA APRGACGHWI 

       550        560        570        580        590        600 
RIKDGKIENY QCVVPTTWNG SPRDSKGQIG AFEASLMNTP MAKPEEPVEI LRTVHSFDPC 

       610        620 
LACSTHVIRP DGQERVVVKV R

P33374 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools