ID   MBHS_ALCHY              Reviewed;         363 AA.
AC   P33375;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-NOV-2008, entry version 56.
DE   RecName: Full=Uptake hydrogenase small subunit;
DE            EC=1.12.99.6;
DE   AltName: Full=Hydrogenlyase;
DE   AltName: Full=Membrane-bound hydrogenase small subunit;
DE   Flags: Precursor;
GN   Name=hupS;
OS   Alcaligenes hydrogenophilus.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Alcaligenes.
OX   NCBI_TaxID=516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=93193199; PubMed=1294332;
RA   Yagi K., Seto T., Terakado M., Umeda F., Doi T., Imanishi T.,
RA   Miura Y.;
RT   "Nucleotide sequences of membrane-bound hydrogenase gene in
RT   Alcaligenes hydrogenophilus.";
RL   Chem. Pharm. Bull. 40:3292-3296(1992).
CC   -!- FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to
CC       increase the production of ATP and to protect nitrogenase against
CC       inhibition or damage by O(2) under carbon- or phosphate-limited
CC       conditions.
CC   -!- CATALYTIC ACTIVITY: H(2) + A = AH(2).
CC   -!- COFACTOR: Binds 1 3Fe-4S cluster (By similarity).
CC   -!- COFACTOR: Binds 2 4Fe-4S clusters (By similarity).
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- PTM: Predicted to be exported by the Tat system. The position of
CC       the signal peptide cleavage has not been experimentally proven.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small
CC       subunit family.
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DR   EMBL; S56898; AAB25779.1; -; Genomic_DNA.
DR   PIR; JH0775; JH0775.
DR   HSSP; P18187; 1FRF.
DR   GO; GO:0009375; C:ferredoxin hydrogenase complex; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0051538; F:3 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:EC.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to u...; IEA:InterPro.
DR   InterPro; IPR001821; NiFe_hyd_ssu.
DR   InterPro; IPR013634; NiFe_hyd_ssu_N.
DR   InterPro; IPR006137; OxRdtase_q6.
DR   InterPro; IPR006311; Tat.
DR   Pfam; PF08425; NiFe_dehyd_N; 1.
DR   Pfam; PF01058; Oxidored_q6; 1.
DR   PIRSF; PIRSF000310; NiFe_hyd_ssu; 1.
DR   PRINTS; PR00614; NIHGNASESMLL.
DR   TIGRFAMs; TIGR00391; hydA; 1.
DR   TIGRFAMs; TIGR01409; TAT_signal_seq; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   3: Inferred from homology;
KW   3Fe-4S; 4Fe-4S; Cell membrane; Iron; Iron-sulfur; Membrane;
KW   Metal-binding; Oxidoreductase; Signal.
FT   SIGNAL        1     43       Tat-type signal (Potential).
FT   CHAIN        44    363       Uptake hydrogenase small subunit.
FT                                /FTId=PRO_0000013422.
FT   METAL        60     60       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL        63     63       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       158    158       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       192    192       Iron-sulfur 1 (4Fe-4S) (By similarity).
FT   METAL       230    230       Iron-sulfur 2 (4Fe-4S); via pros nitrogen
FT                                (By similarity).
FT   METAL       233    233       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       258    258       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       264    264       Iron-sulfur 2 (4Fe-4S) (By similarity).
FT   METAL       273    273       Iron-sulfur 3 (3Fe-4S) (By similarity).
FT   METAL       292    292       Iron-sulfur 3 (3Fe-4S) (By similarity).
FT   METAL       295    295       Iron-sulfur 3 (3Fe-4S) (By similarity).
SQ   SEQUENCE   363 AA;  39696 MW;  1327F5062D81A61B CRC64;
     MIETFYEVMR RQGISRRSFL KYCSLTATSL GLSPVFVPKI VHAMETKPRT PVLWLHGLEC
     TCCSESFIRS AHPLAKDVVL SMISLDYDDT LMAAAGHQAE AILGEVMTKY KGNYILAVEG
     NPPLNQDGMS CIIGGKPFID QLRHVAKDAK AIISWGSCAS WGCVQAAKAN PTQATPIHKV
     ITDKPIIKVP GCPPIAEVMT GVITYMLTFD RFPELDRQGR PKMFYSQRIH DKCYRRPHFD
     AGQFVESWDD ESARKGYCLY KVGCKGPTTY NACSTTRWNG GTSFPIQSGH GCIGCSEDGF
     WDKGSFYSRL TNIHQFGIEA NADSVGVTAV GVVGAATAAH AAVSAIKRAR HKDAAQDTAA
     TQK
//