[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787; PubMed=8226639 [NCBI, ExPASy, EBI, Israel, Japan] Fischer R.J., Helms J., Duerre P.; "Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis."; J. Bacteriol. 175:6959-6969(1993).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787; PubMed=8300540 [NCBI, ExPASy, EBI, Israel, Japan] Nair R.V., Bennett G.N., Papoutsakis E.T.; "Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824."; J. Bacteriol. 176:871-885(1994).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787; DOI=10.1128/JB.183.16.4823-4838.2001; PubMed=11466286 [NCBI, ExPASy, EBI, Israel, Japan] Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.; "Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum."; J. Bacteriol. 183:4823-4838(2001).

Comments

FUNCTION: This enzyme has probably two activities: ADH, and ACDH.
CATALYTIC ACTIVITY: An alcohol + NAD⁺ = an aldehyde or ketone + NADH.
CATALYTIC ACTIVITY: Acetaldehyde + CoA + NAD⁺ = acetyl-CoA + NADH.
INDUCTION: Induced during switch to solvent production.
SIMILARITY: In the N-terminal section; belongs to the aldehyde dehydrogenase family.
SIMILARITY: In the C-terminal section; belongs to the iron-containing alcohol dehydrogenase family.

Copyright

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Cross-references

Sequence databases

EMBL

X72831; CAA51344.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L14817; AAD04638.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AE001438; AAK76907.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A49346; A49346.

RefSeq

NP_149325.1; -.

3D structure databases

ModBase

P33744.

Enzyme and pathway databases

BioCyc

CACE272562:CAP0162-MON; -.

Ontologies

GO:0008774;	Molecular function: acetaldehyde dehydrogenase (acetylating) activity (inferred from electronic annotation from InterPro).
GO:0004022;	Molecular function: alcohol dehydrogenase activity (inferred from electronic annotation from InterPro).
GO:0046872;	Molecular function: metal ion binding (inferred from electronic annotation from InterPro).
GO:0015976;	Biological process: carbon utilization (inferred from electronic annotation from InterPro).
GO:0006066;	Biological process: cellular alcohol metabolic process (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR016162; Ald_DHase_N.
IPR012079; Bifunc_Ald/AlcDHase.
IPR001670; Fe_AlcDHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.

Pfam

PF00465; Fe-ADH; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000111; ALDH_ADH; 1.

PROSITE

PS00913; ADH_IRON_1; 1.
PS00060; ADH_IRON_2; FALSE_NEG.

ProtoNet

P33744.

Genome annotation databases

GeneID

1116167; -.

GenomeReviews

AE001438_GR; CA_P0162.

KEGG

cac:CA_P0162; -.

NMPDR

fig|272562.1.peg.160; -.

Phylogenomic databases

HOGENOM

P33744; -.

Genome annotation databases

CMR

P33744; CA_P0162.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Multifunctional enzyme; NAD; Oxidoreductase; Plasmid.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 862`	`862`	`Aldehyde-alcohol dehydrogenase.`	`PRO_0000087839`
`NP_BIND`	`420 425`	`6`	`NAD (Potential).`
`ACT_SITE`	`244 244`		`By similarity.`

Sequence information

Length: 862 AA [This is the length of the unprocessed precursor]

Molecular weight: 95321 Da [This is the MW of the unprocessed precursor]

CRC64: BE09E32B28DD08B0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKVTTVKELD EKLKVIKEAQ KKFSCYSQEM VDEIFRNAAM AAIDARIELA KAAVLETGMG 

        70         80         90        100        110        120 
LVEDKVIKNH FAGEYIYNKY KDEKTCGIIE RNEPYGITKI AEPIGVVAAI IPVTNPTSTT 

       130        140        150        160        170        180 
IFKSLISLKT RNGIFFSPHP RAKKSTILAA KTILDAAVKS GAPENIIGWI DEPSIELTQY 

       190        200        210        220        230        240 
LMQKADITLA TGGPSLVKSA YSSGKPAIGV GPGNTPVIID ESAHIKMAVS SIILSKTYDN 

       250        260        270        280        290        300 
GVICASEQSV IVLKSIYNKV KDEFQERGAY IIKKNELDKV REVIFKDGSV NPKIVGQSAY 

       310        320        330        340        350        360 
TIAAMAGIKV PKTTRILIGE VTSLGEEEPF AHEKLSPVLA MYEADNFDDA LKKAVTLINL 

       370        380        390        400        410        420 
GGLGHTSGIY ADEIKARDKI DRFSSAMKTV RTFVNIPTSQ GASGDLYNFR IPPSFTLGCG 

       430        440        450        460        470        480 
FWGGNSVSEN VGPKHLLNIK TVAERRENML WFRVPHKVYF KFGCLQFALK DLKDLKKKRA 

       490        500        510        520        530        540 
FIVTDSDPYN LNYVDSIIKI LEHLDIDFKV FNKVGREADL KTIKKATEEM SSFMPDTIIA 

       550        560        570        580        590        600 
LGGTPEMSSA KLMWVLYEHP EVKFEDLAIK FMDIRKRIYT FPKLGKKAML VAITTSAGSG 

       610        620        630        640        650        660 
SEVTPFALVT DNNTGNKYML ADYEMTPNMA IVDAELMMKM PKGLTAYSGI DALVNSIEAY 

       670        680        690        700        710        720 
TSVYASEYTN GLALEAIRLI FKYLPEAYKN GRTNEKAREK MAHASTMAGM ASANAFLGLC 

       730        740        750        760        770        780 
HSMAIKLSSE HNIPSGIANA LLIEEVIKFN AVDNPVKQAP CPQYKYPNTI FRYARIADYI 

       790        800        810        820        830        840 
KLGGNTDEEK VDLLINKIHE LKKALNIPTS IKDAGVLEEN FYSSLDRISE LALDDQCTGA 

       850        860 
NPRFPLTSEI KEMYINCFKK QP

P33744 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools