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UniProtKB/Swiss-Prot entry P33898


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name G3P3_ECOLI
Primary accession number P33898
Secondary accession numbers P76094 P78062 P78291 Q03850 Q63208
Integrated into Swiss-Prot on February 1, 1994
Sequence was last modified on April 27, 2001 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 80)
Name and origin of the protein
Protein name Putative glyceraldehyde-3-phosphate dehydrogenase C
Synonyms GAPDH-C
EC 1.2.1.12
Gene name
Name: gapC
OrderedLocusNames: b4493, JW1413/JW5906
ORFNames: b1416/b1417
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 5: Uncertain;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
PubMed=9019141 [NCBI, ExPASy, EBI, Israel, Japan]
Hidalgo E., Limon A., Aguilar J.;
"A second Escherichia coli gene with similarity to gapA.";
Microbiologia 12:99-106(1996).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Clinical isolate, and River isolate;
PubMed=9622357 [NCBI, ExPASy, EBI, Israel, Japan]
Espinosa-Urgel M., Kolter R.;
"Escherichia coli genes expressed preferentially in an aquatic environment.";
Mol. Microbiol. 28:325-332(1998).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.6.363; PubMed=9097039 [NCBI, ExPASy, EBI, Israel, Japan]
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.";
DNA Res. 3:363-377(1996).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 306-332.
STRAIN=K12;
PubMed=1917845 [NCBI, ExPASy, EBI, Israel, Japan]
Hidalgo E., Chen Y.-M., Lin E.C.C., Aguilar J.;
"Molecular cloning and DNA sequencing of the Escherichia coli K-12 ald gene encoding aldehyde dehydrogenase.";
J. Bacteriol. 173:6118-6123(1991).
[7]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13.
STRAIN=K12;
DOI=10.1007/BF00322437; PubMed=2836696 [NCBI, ExPASy, EBI, Israel, Japan]
Nakamura H., Murakami H., Yamato I., Anraku Y.;
"Nucleotide sequence of the cybB gene encoding cytochrome b561 in Escherichia coli K12.";
Mol. Gen. Genet. 212:1-5(1988).
[8]
NUCLEOTIDE SEQUENCE [MRNA] OF 1-165.
Krawetz S.A.;
Submitted (OCT-1990) to the EMBL/GenBank/DDBJ databases.
[9]
NUCLEOTIDE SEQUENCE [MRNA] OF 1-52.
PubMed=3780374 [NCBI, ExPASy, EBI, Israel, Japan]
Krawetz S.A., Connor W., Cannon P.D., Dixon G.H.;
"A vector-primer-cloner-sequencer plasmid for the construction of cDNA libraries: evidence for a rat glyceraldehyde-3-phosphate dehydrogenase-like mRNA and a ferritin mRNA within testis.";
DNA 5:427-435(1986).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L09067; AAA23856.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
AP009048; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
M64541; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
X07569; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
X54798; CAA38569.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M14166; AAA41178.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S14477; S14477.
3D structure databases
HSSP P17721; 1HDG. [HSSP ENTRY / PDB]
ModBase P33898.
Protein-protein interaction databases
DIP DIP:9737N; -.
Enzyme and pathway databases
BioCyc EcoCyc:EG12103-MON; -.
EcoCyc:G8205-MON; -.
Organism-specific databases
EchoBASE EB2027; -.
EcoGene EG12103; gapC.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0004365; Molecular function: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity (inferred from electronic annotation from InterPro).
GO:0051287; Molecular function: NAD binding (inferred from electronic annotation from InterPro).
GO:0006096; Biological process: glycolysis (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR000173; GlycerAld_3-P_DHase.
IPR006424; Glyceraldehyde-3-P_DHase_1.
Graphical view of domain structure.
PANTHER PTHR10836; GAP_DH; 1.
Pfam PF02800; Gp_dh_C; 1.
PF00044; Gp_dh_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000149; GAP_DH; 1.
PRINTS PR00078; G3PDHDRGNASE.
TIGRFAMs TIGR01534; GAPDH-I; 1.
PROSITE PS00071; GAPDH; 1.
ProtoNet P33898.
Genome annotation databases
GenomeReviews AP009048_GR; b4493.
Phylogenomic databases
HOGENOM P33898; -.
Genome annotation databases
CMR P33898; b4493.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Glycolysis; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   333  333     Putative glyceraldehyde-3-phosphate dehydrogenase C. PRO_0000145649
NP_BIND   11    12  2     NAD (By similarity). 
REGION   149   151  3     Glyceraldehyde 3-phosphate binding (By similarity). 
REGION   209   210  2     Glyceraldehyde 3-phosphate binding (By similarity). 
ACT_SITE   150   150        Nucleophile (By similarity). 
BINDING   34    34        NAD (By similarity). 
BINDING   78    78        NAD; via carbonyl oxygen (By similarity). 
BINDING   180   180        Glyceraldehyde 3-phosphate (By similarity). 
BINDING   232   232        Glyceraldehyde 3-phosphate (By similarity). 
BINDING   315   315        NAD (By similarity). 
SITE   177   177  1     Activates thiol group during catalysis (By similarity). 
CONFLICT   39    39        K -> Y (in Ref. 8 and 9). 
Sequence information
Length: 333 AA [This is the length of the unprocessed precursor] Molecular weight: 35650 Da [This is the MW of the unprocessed precursor] CRC64: E88223297376B0A0 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSKVGINGFG RIGRLVLGRL LEVKSNIDVV AINDLTSPKI LAYLLKHDSN YGPFPWSVDF 

        70         80         90        100        110        120 
TEDSLIVDGK SIAVYAEKEA KNIPWKAKGA EIIVECTGFY TSAEKSQAHL DAGAKKVLIS 

       130        140        150        160        170        180 
APAGEMKTIV YNVNDDTLDG NDTIVSVASC TTNCLAPMAK ALHDSFGIEV GTMTTIHAYT 

       190        200        210        220        230        240 
GTQSLVDGPR GKDLRASRAA AENIIPHTTG AAKAIGLVIP ELSGKLKGHA QRVPVKTGSV 

       250        260        270        280        290        300 
TELVSILGKK VTAEEVNNAL KQATTNNESF GYTDEEIVSS DIIGSHFGSV FDATQTEITA 

       310        320        330 
VGDLQLVKTV AWYDNEYGFV TQLIRTLEKF AKL 

P33898 in FASTA format

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