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UniProtKB/Swiss-Prot entry P34105

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAOX_POPTR
Primary accession number P34105
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1994
Sequence was last modified on February 1, 1996 (Sequence version 3)
Annotations were last modified on    November 25, 2008 (Entry version 56)
Name and origin of the protein
Protein name NADP-dependent malic enzyme
Synonyms NADP-ME
EC 1.1.1.40
Gene name None
From
Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp. trichocarpa) [TaxID: 3694] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids I; Malpighiales; Salicaceae; Saliceae; Populus.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Leaf;
PubMed=16668348 [NCBI, ExPASy, EBI, Israel, Japan]
van Doorsselaere J., Villarroel R., van Montagu M., Inze D.;
"Nucleotide sequence of a cDNA encoding malic enzyme from poplar.";
Plant Physiol. 96:1385-1386(1991).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X56233; CAA39690.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S18826; S18826.
3D structure databases
HSSP P40927; 1GQ2. [HSSP ENTRY / PDB]
ModBase P34105.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0004473; Molecular function: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity (inferred from electronic annotation from EC).
GO:0046872; Molecular function: metal ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051287; Molecular function: NAD binding (inferred from electronic annotation from InterPro).
GO:0006108; Biological process: malate metabolic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
ProtoNet P34105.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cytoplasm; Metal-binding; NADP; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   591  591     NADP-dependent malic enzyme. PRO_0000160203
NP_BIND   335   351  17     NADP (By similarity). 
ACT_SITE   139   139        Proton donor (By similarity). 
ACT_SITE   210   210        Proton acceptor (By similarity). 
METAL   282   282        Divalent metal cation (By similarity). 
METAL   283   283        Divalent metal cation (By similarity). 
METAL   306   306        Divalent metal cation (By similarity). 
BINDING   192   192        NAD (By similarity). 
BINDING   306   306        NAD (By similarity). 
BINDING   447   447        NAD (By similarity). 
SITE   306   306  1     Important for activity (By similarity). 
Sequence information
Length: 591 AA [This is the length of the unprocessed precursor] Molecular weight: 65223 Da [This is the MW of the unprocessed precursor] CRC64: 18DF2433610F4F5A [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MESTLKEMRD GASVLDMDPK STVGGGVEDV YGEDRATEDQ LVTPWTISVA SGYTLLRDPH 

        70         80         90        100        110        120 
HNKGLAFTEK ERDAHYLRGL LPPTTISQQL QEKKLMNTIR QYQLPLQKYT AMMELEERNE 

       130        140        150        160        170        180 
RLFYKLLIDN VEELLPVVYT PTVGEACQKY GSIFKRPQGL YISLKEKGKV LDVLKNWPQK 

       190        200        210        220        230        240 
SIQVIVVTDG ERILGLGDLG CQGIGIPVGK LSLYTALGGV RPSACLPVTI DVGTNNEQLL 

       250        260        270        280        290        300 
KDEFYIGLRQ RRATGQEYSE LLHEFMTAVK QNYGEKVLIQ FEDFANHNAF DLLAKYGTTH 

       310        320        330        340        350        360 
LVFNDDIQGT AAVVLAGLIS ALKLLGGSLA DHTFLFLGAG EAGTGIAELI ALEMSRRSKT 

       370        380        390        400        410        420 
PLEETRKKIW LTDSKGLIVS SRKESLQHFK KPWAHEHEPV KGLLEVVKAI KPIVLIGTSG 

       430        440        450        460        470        480 
VGKTFTKEVI EAMASFNEKP LILALSNPTS QSECTAQEAY TWTKGKAIFA SGSPFDPVEY 

       490        500        510        520        530        540 
EGKVFVPGQS NNAYIFPGLG LGLVISGAIR VHDDMLLAAA EALAGQIKEE YLAKGLIYPP 

       550        560        570        580        590 
LSNIRKISVQ IAANVAAKAY ELGLATRLPR PENLVKHAES CMYSPAYRYY R
P34105 in FASTA format

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