NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=AX3;
De Lozanne A.;
"The dictyostelium HMGCoA reductase genes.";
Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases.

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=AX4;
DOI=10.1038/nature03481; PubMed=15875012 [NCBI, ExPASy, EBI, Israel, Japan]
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
"The genome of the social amoeba Dictyostelium discoideum.";
Nature 435:43-57(2005).

Comments

FUNCTION: This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of the sterol biosynthesis.
CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP⁺ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3 .
PATHWAY: Context: Cholesterol biosynthesis .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane.
SIMILARITY: Belongs to the HMG-CoA reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L19349; AAA33214.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AAFI02000005; EAL71921.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

XP_646489.1; -.

3D structure databases

HSSP

P04035; 1HWI. [HSSP ENTRY / PDB]

ModBase

P34135.

Organism-specific databases

dictyBase

DDB0191125; hmgA.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR002202; HMG_CoA_Rdtase_cat.
IPR004554; HMG_CoA_Rdtase_I_cat.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.770.10; HMG-CoA_red; 1.

PANTHER

PTHR10572; HMG-CoA_red; 1.

Pfam

PF00368; HMG-CoA_red; 1.
Pfam graphical view of domain structure.

PRINTS

PR00071; HMGCOARDTASE.

TIGRFAMs

TIGR00533; HMG_CoA_R_NADP; 1.

PROSITE

PS00066; HMG_COA_REDUCTASE_1; 1.
PS00318; HMG_COA_REDUCTASE_2; 1.
PS01192; HMG_COA_REDUCTASE_3; 1.
PS50065; HMG_COA_REDUCTASE_4; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P34135.

Genome annotation databases

GeneID

3397705; -.

KEGG

ddi:DDB_0191125; -.

Other

ProtoNet

P34135.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cholesterol biosynthesis; Complete proteome; Endoplasmic reticulum; Glycoprotein; Lipid synthesis; Membrane; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 552`	`552`	`3-hydroxy-3-methylglutaryl-coenzyme A reductase 1.`	`PRO_0000114427`
`ACT_SITE`	`237 237`		`Charge relay system (By similarity).`
`ACT_SITE`	`369 369`		`Charge relay system (By similarity).`
`ACT_SITE`	`445 445`		`Charge relay system (By similarity).`
`ACT_SITE`	`543 543`		`Proton donor (By similarity).`
`CARBOHYD`	`288 288`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`375 375`		`N-linked (GlcNAc...) (Potential).`

Sequence information

Length: 552 AA [This is the length of the unprocessed precursor]

Molecular weight: 60387 Da [This is the MW of the unprocessed precursor]

CRC64: 3535CF268CDCE693 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLFAPPNLET KELFWIIYIL ILIPKVFAKV MSVRELFPFF KWGFNIRRSN FLVPILSNNV 

        70         80         90        100        110        120 
IVTGEEAVQY EKPLPYIPQH NQQQQQKQQP SQDYIQQPQN DNNINSGKEQ EQQQQQQQQQ 

       130        140        150        160        170        180 
QQTPDITNQP TKTNKKIPIK ELSNEEILIK LEKGEVLAYR LENELGDCSR AVEIRRMLLE 

       190        200        210        220        230        240 
KQLSKKIEPI PHEGFDFAKV QGQCCENVIG YVPIPVGTAG PIQLNGQLVT IPMATTEGCL 

       250        260        270        280        290        300 
VASTHRGCKA ITESGGAKCT ITSRGMTRAP VVRFSDIVKA SEFVSWINDT DNYQALKAVF 

       310        320        330        340        350        360 
DSTSRFARLS AIKCTIAGRS VYIRFKCDTG DAMGMNMVSK GVEAVLEHLK IIFDDMTLLS 

       370        380        390        400        410        420 
ISGNMCTDKK PSSINWTEGR GRSVVCEAMI TGDVVQRVLK TNVQALVDLN IAKNLIGSAM 

       430        440        450        460        470        480 
AGSIGGFNAH ASNIVTAIFL ATGQDCAQNV ESSNCITQME ACNDGQDLYI TVTMPSIEVG 

       490        500        510        520        530        540 
TVGGGTSLPA QSACLDIIGV KGSSSSKPGA NADQLAKTIA SAVMAGELSL MSALSAGHLM 

       550 
KSHLQYNRAK TN

P34135 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools