ID   HMDH1_DICDI             Reviewed;         552 AA.
AC   P34135; Q55CJ2;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-NOV-2008, entry version 57.
DE   RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase 1;
DE            Short=HMG-CoA reductase 1;
DE            EC=1.1.1.34;
GN   Name=hmgA; ORFNames=DDB_G0269142;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=AX3;
RA   De Lozanne A.;
RT   "The dictyostelium HMGCoA reductase genes.";
RL   Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: This transmembrane glycoprotein is involved in the
CC       control of cholesterol biosynthesis. It is the rate-limiting
CC       enzyme of the sterol biosynthesis.
CC   -!- CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP(+) = (S)-3-
CC       hydroxy-3-methylglutaryl-CoA + 2 NADPH.
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid
CC       biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane.
CC   -!- SIMILARITY: Belongs to the HMG-CoA reductase family.
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DR   EMBL; L19349; AAA33214.1; -; mRNA.
DR   EMBL; AAFI02000005; EAL71921.1; -; Genomic_DNA.
DR   RefSeq; XP_646489.1; -.
DR   HSSP; P04035; 1HWI.
DR   GeneID; 3397705; -.
DR   KEGG; ddi:DDB_0191125; -.
DR   dictyBase; DDB_G0269142; hmgA.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:InterPro.
DR   GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH)...; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR   GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002202; HMG_CoA_Rdtase_cat.
DR   InterPro; IPR004554; HMG_CoA_Rdtase_I_cat.
DR   Gene3D; G3DSA:3.90.770.10; HMG-CoA_red; 1.
DR   PANTHER; PTHR10572; HMG-CoA_red; 1.
DR   Pfam; PF00368; HMG-CoA_red; 1.
DR   PRINTS; PR00071; HMGCOARDTASE.
DR   TIGRFAMs; TIGR00533; HMG_CoA_R_NADP; 1.
DR   PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR   PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR   PROSITE; PS01192; HMG_COA_REDUCTASE_3; 1.
DR   PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE   2: Evidence at transcript level;
KW   Cholesterol biosynthesis; Complete proteome; Endoplasmic reticulum;
KW   Glycoprotein; Lipid synthesis; Membrane; NADP; Oxidoreductase;
KW   Steroid biosynthesis; Sterol biosynthesis.
FT   CHAIN         1    552       3-hydroxy-3-methylglutaryl-coenzyme A
FT                                reductase 1.
FT                                /FTId=PRO_0000114427.
FT   ACT_SITE    237    237       Charge relay system (By similarity).
FT   ACT_SITE    369    369       Charge relay system (By similarity).
FT   ACT_SITE    445    445       Charge relay system (By similarity).
FT   ACT_SITE    543    543       Proton donor (By similarity).
FT   CARBOHYD    288    288       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    375    375       N-linked (GlcNAc...) (Potential).
SQ   SEQUENCE   552 AA;  60387 MW;  3535CF268CDCE693 CRC64;
     MLFAPPNLET KELFWIIYIL ILIPKVFAKV MSVRELFPFF KWGFNIRRSN FLVPILSNNV
     IVTGEEAVQY EKPLPYIPQH NQQQQQKQQP SQDYIQQPQN DNNINSGKEQ EQQQQQQQQQ
     QQTPDITNQP TKTNKKIPIK ELSNEEILIK LEKGEVLAYR LENELGDCSR AVEIRRMLLE
     KQLSKKIEPI PHEGFDFAKV QGQCCENVIG YVPIPVGTAG PIQLNGQLVT IPMATTEGCL
     VASTHRGCKA ITESGGAKCT ITSRGMTRAP VVRFSDIVKA SEFVSWINDT DNYQALKAVF
     DSTSRFARLS AIKCTIAGRS VYIRFKCDTG DAMGMNMVSK GVEAVLEHLK IIFDDMTLLS
     ISGNMCTDKK PSSINWTEGR GRSVVCEAMI TGDVVQRVLK TNVQALVDLN IAKNLIGSAM
     AGSIGGFNAH ASNIVTAIFL ATGQDCAQNV ESSNCITQME ACNDGQDLYI TVTMPSIEVG
     TVGGGTSLPA QSACLDIIGV KGSSSSKPGA NADQLAKTIA SAVMAGELSL MSALSAGHLM
     KSHLQYNRAK TN
//