ID   HMDH2_DICDI             Reviewed;         526 AA.
AC   P34136; Q551D5;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 2.
DT   25-NOV-2008, entry version 57.
DE   RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase 2;
DE            Short=HMG-CoA reductase 2;
DE            EC=1.1.1.34;
GN   Name=hmgB; ORFNames=DDB_G0276615;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 46-526.
RC   STRAIN=AX3;
RA   De Lozanne A.;
RT   "The dictyostelium HMGCoA reductase genes.";
RL   Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: This transmembrane glycoprotein is involved in the
CC       control of cholesterol biosynthesis. It is the rate-limiting
CC       enzyme of the sterol biosynthesis.
CC   -!- CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP(+) = (S)-3-
CC       hydroxy-3-methylglutaryl-CoA + 2 NADPH.
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid
CC       biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
CC   -!- SIMILARITY: Belongs to the HMG-CoA reductase family.
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DR   EMBL; AAFI02000016; EAL69120.1; -; Genomic_DNA.
DR   EMBL; L19350; AAA33215.1; -; mRNA.
DR   RefSeq; XP_643058.1; -.
DR   HSSP; P04035; 1HWI.
DR   GeneID; 3394199; -.
DR   KEGG; ddi:DDB_0215357; -.
DR   dictyBase; DDB_G0276615; hmgB.
DR   GO; GO:0016021; C:integral to membrane; IEA:InterPro.
DR   GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH)...; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0006695; P:cholesterol biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR   GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002202; HMG_CoA_Rdtase_cat.
DR   InterPro; IPR004554; HMG_CoA_Rdtase_I_cat.
DR   Gene3D; G3DSA:3.90.770.10; HMG-CoA_red; 2.
DR   PANTHER; PTHR10572; HMG-CoA_red; 2.
DR   Pfam; PF00368; HMG-CoA_red; 2.
DR   PRINTS; PR00071; HMGCOARDTASE.
DR   TIGRFAMs; TIGR00533; HMG_CoA_R_NADP; 1.
DR   PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR   PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR   PROSITE; PS01192; HMG_COA_REDUCTASE_3; 1.
DR   PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE   2: Evidence at transcript level;
KW   Cholesterol biosynthesis; Complete proteome; Lipid synthesis; NADP;
KW   Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis.
FT   CHAIN         1    526       3-hydroxy-3-methylglutaryl-coenzyme A
FT                                reductase 2.
FT                                /FTId=PRO_0000114428.
FT   ACT_SITE    193    193       Charge relay system (By similarity).
FT   ACT_SITE    325    325       Charge relay system (By similarity).
FT   ACT_SITE    401    401       Charge relay system (By similarity).
FT   ACT_SITE    499    499       Proton donor (By similarity).
SQ   SEQUENCE   526 AA;  56160 MW;  5C95CCBB7180EC92 CRC64;
     MIRIGSNLIK QSIKNTNKSG ISRFFTTGSN NSTNYKPESC VKEEPKGKSV NVEDLKDQEI
     IALVDKGEIQ PHNLETRLPN NFQRAVHIRR KLLARDLQKE HQRALHAQAV VAAAEKAATS
     GEDPSSIQPV VPPTSNLDFE GSLTNLPVDH FDYTKVLGAC CENVIGYIPI PVGVAGPILL
     DGKLVSIPMA TTEGCLVAST HRGAKAITKS GGAKTVLLQS GMTRAPVCRL PSSIRAGELK
     QWIENQENFY QVASAFNSTS RFARLKSIKV VIAGRLVYLR FKSSTGDAMG MNMVSKGVEK
     ALEVITEYFP EMEVLSLSGN VCTDKKPSSI NWLEGRGKSV VAEAVISGDI VRDVLKTTVE
     ALVSLNIDKN LIGSAMAGSI GGFNAHASNI VTALYIATGQ DPAQNVESSN CITLMESING
     GKDLYISVTM PSIEVGTVGG GTHLPAQSAC LDLLKIRGAN LERPGANSEQ LARVVAAAVL
     SGELSLMSAL AAGHLVRSHL KHNRKTEAPA PQADTISMTH NLPHSD
//