ID   G3PC_PINSY              Reviewed;         340 AA.
AC   P34924;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-NOV-2008, entry version 55.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase, cytosolic;
DE            EC=1.2.1.12;
GN   Name=GAPC;
OS   Pinus sylvestris (Scots pine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Coniferopsida; Coniferales; Pinaceae; Pinus; Pinus.
OX   NCBI_TaxID=3349;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=93196483; PubMed=8095691;
RA   Martin W., Lydiate D., Brinkmann H., Forkmann G., Saedler H.,
RA   Cerff R.;
RT   "Molecular phylogenies in angiosperm evolution.";
RL   Mol. Biol. Evol. 10:140-162(1993).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic
CC       form which participates in glycolysis and two chloroplast forms
CC       which participates in photosynthesis. These three forms are
CC       encoded by distinct genes.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; L07501; AAA33779.1; -; mRNA.
DR   PIR; T09663; T09663.
DR   HSSP; P00357; 4GPD.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DHase.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DHase_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   ProDom; PD007761; GAPDH_like; 1.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    340       Glyceraldehyde-3-phosphate dehydrogenase,
FT                                cytosolic.
FT                                /FTId=PRO_0000145614.
FT   NP_BIND      16     17       NAD (By similarity).
FT   REGION      156    158       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      216    217       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    157    157       Nucleophile (By similarity).
FT   BINDING      38     38       NAD (By similarity).
FT   BINDING      85     85       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     187    187       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     239    239       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     321    321       NAD (By similarity).
FT   SITE        184    184       Activates thiol group during catalysis
FT                                (By similarity).
SQ   SEQUENCE   340 AA;  36530 MW;  1E81ACA460086F01 CRC64;
     MGSTGKIKIG INGFGRIGRL VARVALTRDD IELVGVNDPF ISTDYMSYMF KYDSVHGKWK
     HHEVNVKDSK TLLFGEKSVA VFGCRNPEEI PWGEVGAEYV VESTGVFTDK EKASAHLKAG
     AKKVVISAPS KDAPMFVVGV NEHQYKSDVN IVSNASCTTN CLAPLAKVIN DKFGIVEGLM
     TTVHSITATQ KTVDGPSNKD WRGGRGAGFN IIPSSTGAAK AVGKVLPALN GKLTGMAFRV
     PTPDVSVVDL TVRLEKSATY DEIKAAIKAE SEGNLKGILG YTEDAVVSTD FIGDSRSSIF
     DAQAGIALSD NFVKLVSWYD NEWGYSSRVV DLIVHMAATQ
//