ID   CRAC_DICDI              Reviewed;         698 AA.
AC   P35401; Q54NJ7;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 4.
DT   25-NOV-2008, entry version 41.
DE   RecName: Full=Protein CRAC;
GN   Name=dagA; ORFNames=DDB_G0285161;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AX4;
RX   MEDLINE=94375528; PubMed=8089184; DOI=10.1083/jcb.126.6.1537;
RA   Insall R., Kupsa A., Lilly P.J., Shaulsky G., Levin L.R., Loomis W.F.,
RA   Devreotes P.N.;
RT   "CRAC, a cytosolic protein containing a pleckstrin homology domain, is
RT   required for receptor and G protein-mediated activation of adenylyl
RT   cyclase in Dictyostelium.";
RL   J. Cell Biol. 126:1537-1545(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A.,
RA   Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q.,
RA   Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F.,
RA   Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P.,
RA   Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P.,
RA   Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N.,
RA   Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M.,
RA   Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I.,
RA   Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R.,
RA   Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C.,
RA   Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D.,
RA   Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S.,
RA   Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T.,
RA   Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A.,
RA   Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M.,
RA   Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G.,
RA   Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-9.
RX   MEDLINE=94245733; PubMed=8188693;
RA   Lilly P.J., Devreotes P.N.;
RT   "Identification of CRAC, a cytosolic regulator required for guanine
RT   nucleotide stimulation of adenylyl cyclase in Dictyostelium.";
RL   J. Biol. Chem. 269:14123-14129(1994).
CC   -!- FUNCTION: Couples activated G protein to adenylyl cyclase signal
CC       transduction from surface cAMP receptor.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DEVELOPMENTAL STAGE: Tightly developmentally regulated.
CC   -!- SIMILARITY: Contains 1 PH domain.
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DR   EMBL; U06228; AAA61782.1; -; Genomic_DNA.
DR   EMBL; AAFI02000075; EAL64837.1; -; Genomic_DNA.
DR   PIR; A54796; A54796.
DR   RefSeq; XP_638365.1; -.
DR   GeneID; 3389921; -.
DR   KEGG; ddi:DDB_0191434; -.
DR   dictyBase; DDB_G0285161; dagA.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0031256; C:leading edge membrane; IDA:dictyBase.
DR   GO; GO:0008047; F:enzyme activator activity; IMP:dictyBase.
DR   GO; GO:0005547; F:phosphatidylinositol-3,4,5-triphosphate bin...; IDA:dictyBase.
DR   GO; GO:0043325; F:phosphatidylinositol-3,4-bisphosphate binding; IDA:dictyBase.
DR   GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR   GO; GO:0006935; P:chemotaxis; IMP:dictyBase.
DR   GO; GO:0007188; P:G-protein signaling, coupled to cAMP nucleo...; IDA:dictyBase.
DR   GO; GO:0048015; P:phosphoinositide-mediated signaling; TAS:dictyBase.
DR   GO; GO:0045762; P:positive regulation of adenylate cyclase ac...; IMP:dictyBase.
DR   GO; GO:0008064; P:regulation of actin polymerization or depol...; IMP:dictyBase.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cel...; TAS:dictyBase.
DR   InterPro; IPR001849; PH.
DR   InterPro; IPR011993; PH_type.
DR   Gene3D; G3DSA:2.30.29.30; PH_type; 1.
DR   Pfam; PF00169; PH; 1.
DR   SMART; SM00233; PH; 1.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Cytoplasm; Direct protein sequencing.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    698       Protein CRAC.
FT                                /FTId=PRO_0000079325.
FT   DOMAIN       22    122       PH.
FT   COMPBIAS    350    363       Poly-Thr.
FT   COMPBIAS    468    480       Poly-Gly.
FT   COMPBIAS    613    624       Poly-Ser.
FT   CONFLICT    653    653       R -> G (in Ref. 1; AAA61782).
SQ   SEQUENCE   698 AA;  78630 MW;  3CC3D8FF6D9D849B CRC64;
     MGKTERKKEL LELFEYEKIK GDVSYSSIMK KAGGNGKGFL DRYFALHRNY ILYYKLGKSS
     LKPDDKQEPQ GYINLMDCNP DDTKEIAPLM FQISHKHRTY IVKAKDESSM KQFLTLLIAR
     IRSLEKIDID KLGCTVVVLT KVKKFREVLT NPLILPDRVS PEMAEEWVKQ MKNYNASFNL
     ADPFIKQVEQ ISEFCRGEVK EYIDWFGGPE GPRLAMIRCE ETVLSNWVEY INKTSSEITT
     YQDNRFFRED FKDIAVHLKN MTTFIDCYND YMIHCRKYNN NKPNTKFLEE KQTFKEYIEK
     FIPKVASCND VSLNQFYDRS LIQSSDGIVT INTSGIKKTL INQSNIISIT STTTTTTTTT
     TTTCSMPNMS NLIHSLDHTN LNIIDLNHSK SQQQLHPPPS PHHQHLHHQI VSNSKDFNIS
     VSSNNFNDGN SEFPNLDINC DFDLTSASNL SSPILSSEVP SNVVDPIGSG QGGGGSGGGG
     VTAVTEEAIN EKWHFDCNTS MIFKPPSEDG RNEGSNMSTS SITSKMSLSL NGGFDMKWVY
     QCGYFKSKNM GSISWNGKHW CWSHPRTSYK IKYIWDPTKQ SFLNIPFKSR VGATGGGSVP
     SSQSTNNLQS STSSMSSLSS SSTSTTKRSH PTTLYPDYQF KDNLLTPIII EGRHQPSLTL
     IDSPLTIPNA CLLTIAMTQY IQDALIHLSL GPKVLSSK
//