[1]	NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 43-58. STRAIN=Sprague-Dawley; TISSUE=Testis; PubMed=8182039 [NCBI, ExPASy, EBI, Israel, Japan] Brown L.J., McDonald M.J., Lehn D.A., Moran S.M.; "Sequence of rat mitochondrial glycerol-3-phosphate dehydrogenase cDNA. Evidence for EF-hand calcium-binding domains."; J. Biol. Chem. 269:14363-14366(1994).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=Wistar; TISSUE=Liver; PubMed=7937996 [NCBI, ExPASy, EBI, Israel, Japan] Mueller S., Seitz H.J.; "Cloning of a cDNA for the FAD-linked glycerol-3-phosphate dehydrogenase from rat liver and its regulation by thyroid hormones."; Proc. Natl. Acad. Sci. U.S.A. 91:10581-10585(1994).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Testis; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + acceptor = glycerone phosphate + reduced acceptor.
COFACTOR: FAD.
ENZYME REGULATION: Calcium-binding enhance the activity of the enzyme.
PATHWAY: Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1 .
SUBCELLULAR LOCATION: Mitochondrion.
SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
SIMILARITY: Contains 2 EF-hand domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U08027; AAB60443.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X78593; CAA55329.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC083565; AAH83565.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

A54051; A54051.

NP_036868.1; -.

3D structure databases

HSSP

P02593; 1CDM. [HSSP ENTRY / SWISS-3DIMAGE / PDB]

ModBase

P35571.

PTM databases

PhosphoSite

P35571; -.

Organism-specific databases

RGD

2726; Gpd2.

Gene expression databases

ArrayExpress

P35571; -.

Ontologies

GO:0009331;	Cellular component: glycerol-3-phosphate dehydrogenase complex (inferred from electronic annotation from InterPro).
GO:0005739;	Cellular component: mitochondrion (inferred from electronic annotation from UniProtKB-KW).
GO:0005509;	Molecular function: calcium ion binding (inferred from electronic annotation from InterPro).
GO:0004368;	Molecular function: glycerol-3-phosphate dehydrogenase activity (inferred from electronic annotation from InterPro).
GO:0006072;	Biological process: glycerol-3-phosphate metabolic process (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR011992; EF-Hand_type.
IPR002048; EF_hand_Ca_bd.
IPR000447; FAD-dep_Gly3P_DHase.
IPR006076; FAD-dep_OxRdtase.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.238.10; EF-Hand_type; 1.

Pfam

PF01266; DAO; 1.
PF00036; efhand; 2.
Pfam graphical view of domain structure.

PRINTS

PR01001; FADG3PDH.

ProDom

PD000012; EF-hand; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00054; EFh; 2.
SMART graphical view of domain structure.

PROSITE

PS00018; EF_HAND_1; 1.
PS50222; EF_HAND_2; 2.
PS00977; FAD_G3PDH_1; 1.
PS00978; FAD_G3PDH_2; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P35571.

Genome annotation databases

Ensembl

ENSRNOG00000033824; Rattus norvegicus. [Contig view]

GeneID

25062; -.

KEGG

rno:25062; -.

Phylogenomic databases

HOVERGEN

P35571; -.

Other

NextBio

605290; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calcium; Direct protein sequencing; FAD; Flavoprotein; Mitochondrion; Oxidoreductase; Phosphoprotein; Repeat; Transit peptide.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 42`	`42`	`Mitochondrion.`
`CHAIN`	`43 727`	`685`	`Glycerol-3-phosphate dehydrogenase, mitochondrial.`	`PRO_0000010431`
`DOMAIN`	`623 658`	`36`	`EF-hand 1.`
`DOMAIN`	`659 694`	`36`	`EF-hand 2.`
`NP_BIND`	`71 99`	`29`	`FAD (Potential).`
`CA_BIND`	`672 683`	`12`	`Potential.`
`MOD_RES`	`600 600`		`Phosphotyrosine (By similarity).`
`MOD_RES`	`601 601`		`Phosphotyrosine (By similarity).`

Sequence information

Length: 727 AA [This is the length of the unprocessed precursor]

Molecular weight: 80973 Da [This is the MW of the unprocessed precursor]

CRC64: B2AAA45E93F5F7CB [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAFQKVVKGT ILMGGGALAT VLGLSQFAHY RRKQVSLAYV EAATCFSEPV NREPPSREAQ 

        70         80         90        100        110        120 
LMTLQNTSEF DILVIGGGAT GCGCALDAVT RGLKTALVER NDFASGTSSR STKLIHGGVR 

       130        140        150        160        170        180 
YLQKAITNLD VEQYRMVKEA LHERANLLEI APHLSAPLPI MLPLYKWWQL PYYWVGIKMY 

       190        200        210        220        230        240 
DLVAGSHCLK SSYVLSKSRA LEHFPMLQKD KLVGAIVYYD GQHNDARMNL AIALTAARYG 

       250        260        270        280        290        300 
AATANYMEVV SLLKKTDPET GKERVSGARC KDVLTGHEFN VRAKCVINAT GPFTDSVRKM 

       310        320        330        340        350        360 
DDNDVVPICQ PSAGVHIVMP GYYSPENMGL LDPATSDGRV IFFLPWEKMT IAGTTDSPTD 

       370        380        390        400        410        420 
VTHHPIPSED DINFILNEVR NYLSCDVEVR RGDVLAAWSG IRPLVTDPKS ANTQSISRNH 

       430        440        450        460        470        480 
VVEVSDSGLI TIAGGKWTTY RSMAEDTVNK AVKLHNLNAG PSRTVGLFLQ GGKDWSPTLY 

       490        500        510        520        530        540 
IRLVQDYGLE SEVAQHLAKT YGDKAFDVAK MASVTGKRWP VVGVRLVSEF PYIEAEVKYG 

       550        560        570        580        590        600 
IKEYACTAVD MISRRTRLAF LNVQAAEEAL PKIVELMGRE LNWSELRKQE ELETATRFLY 

       610        620        630        640        650        660 
YEMGYKSRTE QLTDSTEISL LPPDIDRYKK RFHMFDEDEK GFITIVDVQR VLESINVQMD 

       670        680        690        700        710        720 
EDTLHEILCE VDLNKNGQVE LHEFLQLMSA VHTGRVSGSR LAILMKTAEE NLDRRVPIPV 


DRSCGGL

P35571 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools