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UniProtKB/Swiss-Prot entry P36444

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAOC_FLAPR
Primary accession number P36444
Secondary accession numbers None
Integrated into Swiss-Prot on June 1, 1994
Sequence was last modified on June 1, 1994 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 59)
Name and origin of the protein
Protein name NADP-dependent malic enzyme, chloroplastic [Precursor]
Synonyms NADP-ME
EC 1.1.1.40
Gene name
Name: MODA
From
Flaveria pringlei [TaxID: 4226] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; asterids; campanulids; Asterales; Asteraceae; Asteroideae; Tageteae; Flaveria.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Leaf;
DOI=10.1007/BF00019491; PubMed=7858216 [NCBI, ExPASy, EBI, Israel, Japan]
Lipka B., Steinmueller K., Rosche E., Borsch D., Westhoff P.;
"The C3 plant Flaveria pringlei contains a plastidic NADP-malic enzyme which is orthologous to the C4 isoform of the C4 plant F. trinervia.";
Plant Mol. Biol. 26:1775-1783(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X78069; CAA54986.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S52016; S42939.
3D structure databases
HSSP P40927; 1GQ2. [HSSP ENTRY / PDB]
ModBase P36444.
Ontologies
GO
GO:0009507; Cellular component: chloroplast (inferred from electronic annotation from UniProtKB-KW).
GO:0004473; Molecular function: malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity (inferred from electronic annotation from EC).
GO:0046872; Molecular function: metal ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051287; Molecular function: NAD binding (inferred from electronic annotation from InterPro).
GO:0006108; Biological process: malate metabolic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
ProtoNet P36444.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Chloroplast; Metal-binding; NAD; NADP; Oxidoreductase; Plastid; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1   ?61        Chloroplast. 
CHAIN   ?62   647        NADP-dependent malic enzyme, chloroplastic. PRO_0000018545
NP_BIND   391   407  17     NADP (By similarity). 
ACT_SITE   195   195        Proton donor (By similarity). 
ACT_SITE   266   266        Proton acceptor (By similarity). 
METAL   338   338        Divalent metal cation (By similarity). 
METAL   339   339        Divalent metal cation (By similarity). 
METAL   362   362        Divalent metal cation (By similarity). 
BINDING   248   248        NAD (By similarity). 
BINDING   362   362        NAD (By similarity). 
BINDING   503   503        NAD (By similarity). 
SITE   362   362  1     Important for activity (By similarity). 
Sequence information
Length: 647 AA [This is the length of the unprocessed precursor] Molecular weight: 71204 Da [This is the MW of the unprocessed precursor] CRC64: B06AE314F48BE352 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MMSLNSSSVV KSSISGVSWT QSQSVRLSVR RPMVVAMVNS NGRPERSVGV SVDGAVKDVN 

        70         80         90        100        110        120 
APVAVEVADS ESKKPTAVVG GGVEDVYGED SATEDHFITP WSVSVASGYS LLRDPHHNKG 

       130        140        150        160        170        180 
LAFTEKERDA HYLRGLLPPV VVNHDLQVKK MMHNIRQYEV PLQRYQAMMD LQERNERLFY 

       190        200        210        220        230        240 
KLLIENIEEL LPIVYTPTVG EACQKYGTIF KNPQGLYISL KDKGKVLEIL KNWPQKKIQV 

       250        260        270        280        290        300 
IVVTDGERIL GLGDLGCQGM GIPVGKLSLY TALGGIRPSA CLPITIDVGT NNEKMLNDEF 

       310        320        330        340        350        360 
YIGLRQRRAS GKEYAELMNE FMSAVKQNYG EKVLIQFEDF ANHNAFDLLE KYRTTHLVFN 

       370        380        390        400        410        420 
DDIQGTASVV LAGLISALKL VGGSLADHKF LFLGAGEAGT GIAELIALEI SKQTNAPLEE 

       430        440        450        460        470        480 
TRKKIWLVDS KGLIVRSRLD SLQHFKKPWA HDHEPVNKFL DAVKAIKPTV LIGSSGAGQT 

       490        500        510        520        530        540 
FTKEVVEAMS SFNEKPIILA LSNPTSQSEC TAEQAYTWSE GRTIFASGSP FAPVEYNGKV 

       550        560        570        580        590        600 
YVSGQSNNAY IFPGFGLGLI ISGAIRVHDE MLLAASEALA EQVTQEHFDN GLIYPPFTNI 

       610        620        630        640 
RKISAHIAAK VAAKAYELGL ASRLPQPENL VAYAESCMYS PKYRNYR
P36444 in FASTA format

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