ID HEM6_ECOLI Reviewed; 299 AA. AC P36553; DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot. DT 01-JUN-1994, sequence version 1. DT 25-NOV-2008, entry version 70. DE RecName: Full=Coproporphyrinogen 3 oxidase, aerobic; DE EC=1.3.3.3; DE AltName: Full=Coproporphyrinogen III oxidase, aerobic; DE Short=Coproporphyrinogenase; DE Short=Coprogen oxidase; GN Name=hemF; OrderedLocusNames=b2436, JW2429; OS Escherichia coli (strain K12). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=K12; RX MEDLINE=94131946; PubMed=8300522; RA Troup B., Jahn M., Hungerer C., Jahn D.; RT "Isolation of the hemF operon containing the gene for the Escherichia RT coli aerobic coproporphyrinogen III oxidase by in vivo complementation RT of a yeast HEM13 mutant."; RL J. Bacteriol. 176:673-680(1994). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX MEDLINE=97349980; PubMed=9205837; DOI=10.1093/dnares/4.2.91; RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., RA Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., RA Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., RA Oshima T., Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., RA Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., RA Yamagata S., Horiuchi T.; RT "Construction of a contiguous 874-kb sequence of the Escherichia coli- RT K12 genome corresponding to 50.0-68.8 min on the linkage map and RT analysis of its sequence features."; RL DNA Res. 4:91-113(1997). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains RT MG1655 and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). RN [5] RP CHARACTERIZATION, AND CRYSTALLIZATION. RX PubMed=13129604; DOI=10.1016/S0378-1097(03)00531-7; RA Breckau D., Mahlitz E., Sauerwald A., Layer G., Jahn D.; RT "Oxygen-dependent coproporphyrinogen-III oxidase from Escherichia RT coli: one-step purification and biochemical characterisation."; RL FEMS Microbiol. Lett. 226:31-37(2003). RN [6] RP CHARACTERIZATION, AND MUTAGENESIS OF TRP-36; HIS-96; HIS-106; TRP-123; RP TYR-135; HIS-145; TYR-160; TRP-166; CYS-167; TYR-170; HIS-175; RP TYR-213; TYR-240; TYR-276 AND TRP-298. RX PubMed=12975365; DOI=10.1074/jbc.M308553200; RA Breckau D., Mahlitz E., Sauerwald A., Layer G., Jahn D.; RT "Oxygen-dependent coproporphyrinogen III oxidase (HemF) from RT Escherichia coli is stimulated by manganese."; RL J. Biol. Chem. 278:46625-46631(2003). CC -!- CATALYTIC ACTIVITY: Coproporphyrinogen-III + O(2) + 2 H(+) = CC protoporphyrinogen-IX + 2 CO(2) + 2 H(2)O. CC -!- COFACTOR: Manganese. CC -!- PATHWAY: Porphyrin metabolism; protoporphyrin-IX biosynthesis; CC protoporphyrinogen-IX from coproporphyrinogen III (O2 route): step CC 1/1. CC -!- SUBUNIT: Homodimer. CC -!- INTERACTION: CC P0A6Z3:htpG; NbExp=1; IntAct=EBI-1114572, EBI-369221; CC P18843:nadE; NbExp=1; IntAct=EBI-1114572, EBI-548960; CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- SIMILARITY: Belongs to the aerobic coproporphyrinogen III oxidase CC family. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; X75413; CAA53167.1; -; Genomic_DNA. DR EMBL; U00096; AAC75489.1; -; Genomic_DNA. DR EMBL; AP009048; BAA16319.1; -; Genomic_DNA. DR PIR; B36964; B36964. DR RefSeq; AP_003030.1; -. DR RefSeq; NP_416931.1; -. DR IntAct; P36553; -. DR GeneID; 946908; -. DR GenomeReviews; U00096_GR; b2436. DR GenomeReviews; AP009048_GR; JW2429. DR KEGG; ecj:JW2429; -. DR KEGG; eco:b2436; -. DR EchoBASE; EB2106; -. DR EcoGene; EG12189; hemF. DR HOGENOM; P36553; -. DR BioCyc; EcoCyc:COPROGENOXI-MON; -. DR BioCyc; MetaCyc:COPROGENOXI-MON; -. DR LinkHub; P36553; -. DR GO; GO:0005737; C:cytoplasm; IEA:HAMAP. DR GO; GO:0004109; F:coproporphyrinogen oxidase activity; IEA:HAMAP. DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW. DR GO; GO:0005515; F:protein binding; IPI:IntAct. DR GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW. DR GO; GO:0006779; P:porphyrin biosynthetic process; IEA:HAMAP. DR HAMAP; MF_00333; -; 1. DR InterPro; IPR001260; Coprogen_oxidas. DR Gene3D; G3DSA:3.40.1500.10; Coprogen_oxidas; 1. DR PANTHER; PTHR10755; Coprogen_oxidas; 1. DR Pfam; PF01218; Coprogen_oxidas; 1. DR PIRSF; PIRSF000166; Coproporphyri_ox; 1. DR PRINTS; PR00073; COPRGNOXDASE. DR PROSITE; PS01021; COPROGEN_OXIDASE; 1. PE 1: Evidence at protein level; KW Complete proteome; Cytoplasm; Manganese; Metal-binding; KW Oxidoreductase; Porphyrin biosynthesis. FT CHAIN 1 299 Coproporphyrinogen 3 oxidase, aerobic. FT /FTId=PRO_0000109894. FT METAL 96 96 Manganese. FT METAL 106 106 Manganese. FT METAL 145 145 Manganese. FT METAL 175 175 Manganese. SQ SEQUENCE 299 AA; 34323 MW; 53667E5C7B6D0198 CRC64; MKPDAHQVKQ FLLNLQDTIC QQLTAVDGAE FVEDSWQREA GGGGRSRVLR NGGVFEQAGV NFSHVHGEAM PASATAHRPE LAGRSFEAMG VSLVVHPHNP YVPTSHANVR FFIAEKPGAD PVWWFGGGFD LTPFYGFEED AIHWHRTARD LCLPFGEDVY PRYKKWCDEY FYLKHRNEQR GIGGLFFDDL NTPDFDRCFA FMQAVGKGYT DAYLPIVERR KAMAYGERER NFQLYRRGRY VEFNLVWDRG TLFGLQTGGR TESILMSMPP LVRWEYDYQP KDGSPEAALS EFIKVRDWV //