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UniProtKB/Swiss-Prot entry P37061


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NAOX_ENTFA
Primary accession number P37061
Secondary accession numbers None
Integrated into Swiss-Prot on June 1, 1994
Sequence was last modified on June 1, 1994 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 67)
Name and origin of the protein
Protein name NADH oxidase
Synonyms NOXase
EC 1.6.99.3
Gene name
Name: nox
OrderedLocusNames: EF_1586
From
Enterococcus faecalis (Streptococcus faecalis) [TaxID: 1351] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Lactobacillales; Enterococcaceae; Enterococcus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 11700 / 10C1 / DSM 20409 / NCIB 8661;
DOI=10.1016/0022-2836(92)90215-6; PubMed=1404382 [NCBI, ExPASy, EBI, Israel, Japan]
Ross R.P., Claiborne A.;
"Molecular cloning and analysis of the gene encoding the NADH oxidase from Streptococcus faecalis 10C1. Comparison with NADH peroxidase and the flavoprotein disulfide reductases.";
J. Mol. Biol. 227:658-671(1992).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=V583 / ATCC 700802;
DOI=10.1126/science.1080613; PubMed=12663927 [NCBI, ExPASy, EBI, Israel, Japan]
Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis.";
Science 299:2071-2074(2003).
[3]
PROTEIN SEQUENCE OF 3-17; 34-52 AND 226-250.
STRAIN=ATCC 11700 / 10C1 / DSM 20409 / NCIB 8661;
PubMed=2511195 [NCBI, ExPASy, EBI, Israel, Japan]
Ahmed S.A., Claiborne A.;
"The streptococcal flavoprotein NADH oxidase. I. Evidence linking NADH oxidase and NADH peroxidase cysteinyl redox centers.";
J. Biol. Chem. 264:19856-19863(1989).
[4]
ACTIVE SITE.
PubMed=8262333 [NCBI, ExPASy, EBI, Israel, Japan]
Claiborne A., Miller H., Parsonage D., Ross R.P.;
"Protein-sulfenic acid stabilization and function in enzyme catalysis and gene regulation.";
FASEB J. 7:1483-1490(1993).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X68847; CAA48728.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE016830; AAO81372.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S26965; S26965.
RefSeq NP_815302.1; -.
3D structure databases
HSSP P37062; 1NHP. [HSSP ENTRY / PDB]
ModBase P37061.
Protein family/group databases
PeroxiBase 5456; EfNadOxd01.
Enzyme and pathway databases
BioCyc EFAE226185:EF_1586-MON; -.
Family and domain databases
InterPro IPR000759; Adrndx_reductase.
IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR001100; Pyr_nuc-diS_OxRdtase.
IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
IPR001327; Pyr_OxRdtase_NAD_bd.
Graphical view of domain structure.
Gene3D G3DSA:3.30.390.30; Pyr_redox_dim; 1.
Pfam PF00070; Pyr_redox; 1.
PF07992; Pyr_redox_2; 1.
PF02852; Pyr_redox_dim; 1.
Pfam graphical view of domain structure.
PRINTS PR00419; ADXRDTASE.
PR00368; FADPNR.
PR00411; PNDRDTASEI.
ProDom PD000139; FAD_pyr_redox; 1.
[Domain structure / List of seq. sharing at least 1 domain]
BLOCKS P37061.
Genome annotation databases
GeneID 1200486; -.
GenomeReviews AE016830_GR; EF_1586.
KEGG efa:EF1586; -.
NMPDR fig|226185.1.peg.1487; -.
TIGR EF_1586; -.
Phylogenomic databases
HOGENOM P37061; -.
Other
ProtoNet P37061.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; FAD; Flavoprotein; NAD; Oxidation; Oxidoreductase; Redox-active center.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   446  446     NADH oxidase. PRO_0000184701
NP_BIND   7    11  5     FAD (By similarity). 
NP_BIND   110   113  4     FAD (By similarity). 
NP_BIND   150   165  16     NAD (By similarity). 
NP_BIND   271   281  11     FAD (By similarity). 
ACT_SITE   10    10        Proton acceptor (By similarity). 
ACT_SITE   42    42        Redox-active. 
BINDING   32    32        FAD (By similarity). 
BINDING   42    42        FAD (By similarity). 
BINDING   177   177        NAD (By similarity). 
BINDING   186   186        NAD (By similarity). 
BINDING   243   243        NAD; via amide nitrogen (By similarity). 
BINDING   299   299        FAD; via amide nitrogen (By similarity). 
BINDING   328   328        NAD; via carbonyl oxygen (By similarity). 
MOD_RES   42    42        Cysteine sulfenic acid (-SOH). 
Sequence information
Length: 446 AA [This is the length of the unprocessed precursor] Molecular weight: 48915 Da [This is the MW of the unprocessed precursor] CRC64: D0762A47FC3DC071 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKVVVVGCTH AGTSAVKSIL ANHPEAEVTV YERNDNISFL SCGIALYVGG VVKNAADLFY 

        70         80         90        100        110        120 
SNPEELASLG ATVKMEHNVE EINVDDKTVT AKNLQTGATE TVSYDKLVMT TGSWPIIPPI 

       130        140        150        160        170        180 
PGIDAENILL CKNYSQANVI IEKAKDAKRV VVVGGGYIGI ELVEAFVESG KQVTLVDGLD 

       190        200        210        220        230        240 
RILNKYLDKP FTDVLEKELV DRGVNLALGE NVQQFVADEQ GKVAKVITPS QEFEADMVIM 

       250        260        270        280        290        300 
CVGFRPNTEL LKDKVDMLPN GAIEVNEYMQ TSNPDIFAAG DSAVVHYNPS QTKNYIPLAT 

       310        320        330        340        350        360 
NAVRQGMLVG RNLTEQKLAY RGTQGTSGLY LFGWKIGSTG VTKESAKLNG LDVEATVFED 

       370        380        390        400        410        420 
NYRPEFMPTT EKVLMELVYE KGTQRIVGGQ LMSKYDITQS ANTLSLAVQN KMTVEDLAIS 

       430        440 
DFFFQPHFDR PWNYLNLLAQ AALENM 

P37061 in FASTA format

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