ID   ETR1_YEAST              Reviewed;         380 AA.
AC   P38071; Q6Q5P2;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 3.
DT   25-NOV-2008, entry version 76.
DE   RecName: Full=Enoyl-[acyl-carrier protein] reductase [NADPH, B-specific], mitochondrial;
DE            EC=1.3.1.10;
DE   AltName: Full=Trans-2-enoyl-CoA reductase;
DE            EC=1.3.1.38;
DE   AltName: Full=Mitochondrial respiratory function protein 1;
DE   Flags: Precursor;
GN   Name=ETR1; Synonyms=MRF1, MRF1'; OrderedLocusNames=YBR026C;
GN   ORFNames=YBR0310;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 10-25.
RC   STRAIN=ATCC 26786 / X2180-1A;
RX   MEDLINE=94253088; PubMed=8195160;
RA   Yamazoe M., Shirahige K., Rashid M.B., Kaneko Y., Nakayama T.,
RA   Ogasawara N., Yoshikawa H.;
RT   "A protein which binds preferentially to single-stranded core sequence
RT   of autonomously replicating sequence is essential for respiratory
RT   function in mitochondrial of Saccharomyces cerevisiae.";
RL   J. Biol. Chem. 269:15244-15252(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   MEDLINE=94378725; PubMed=8091864; DOI=10.1002/yea.320100010;
RA   Smits P.H.M., de Haan M., Maat C., Grivell L.A.;
RT   "The complete sequence of a 33 kb fragment on the right arm of
RT   chromosome II from Saccharomyces cerevisiae reveals 16 open reading
RT   frames, including ten new open reading frames, five previously
RT   identified genes and a homologue of the SCO1 gene.";
RL   Yeast 10:S75-S80(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   MEDLINE=95112788; PubMed=7813418;
RA   Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA   Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA   Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA   Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA   Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA   Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J.,
RA   Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C.,
RA   Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M.,
RA   Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L.,
RA   Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J.,
RA   Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T.,
RA   Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A.,
RA   Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B.,
RA   Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I.,
RA   Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M.,
RA   Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A.,
RA   van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I.,
RA   Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H.,
RA   Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.;
RT   "Complete DNA sequence of yeast chromosome II.";
RL   EMBO J. 13:5795-5809(1994).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
RA   Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
RA   Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
RA   Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-
RT   encoding clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   MEDLINE=21393706; PubMed=11502169; DOI=10.1021/bi010277r;
RA   Grandier-Vazeille X., Bathany K., Chaignepain S., Camougrand N.,
RA   Manon S., Schmitter J.-M.;
RT   "Yeast mitochondrial dehydrogenases are associated in a supramolecular
RT   complex.";
RL   Biochemistry 40:9758-9769(2001).
RN   [6]
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX   MEDLINE=21400968; PubMed=11509667;
RX   DOI=10.1128/MCB.21.18.6243-6253.2001;
RA   Torkko J.M., Koivuranta K.T., Miinalainen I.J., Yagi A.I., Schmitz W.,
RA   Kastaniotis A.J., Airenne T.T., Gurvitz A., Hiltunen K.J.;
RT   "Candida tropicalis Etr1p and Saccharomyces cerevisiae Ybr026p
RT   (Mrf1'p), 2-enoyl thioester reductases essential for mitochondrial
RT   respiratory competence.";
RL   Mol. Cell. Biol. 21:6243-6253(2001).
RN   [7]
RP   FUNCTION, AND MUTAGENESIS OF TYR-73.
RX   PubMed=12614607; DOI=10.1016/S0022-2836(03)00038-X;
RA   Airenne T.T., Torkko J.M., Van den plas S., Sormunen R.T.,
RA   Kastaniotis A.J., Wierenga R.K., Hiltunen J.K.;
RT   "Structure-function analysis of enoyl thioester reductase involved in
RT   mitochondrial maintenance.";
RL   J. Mol. Biol. 327:47-59(2003).
RN   [8]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [9]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
RX   MEDLINE=22975177; PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA   Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P.,
RA   Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B.,
RA   Rehling P., Pfanner N., Meisinger C.;
RT   "The proteome of Saccharomyces cerevisiae mitochondria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN   [10]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-135 AND SER-339, AND
RP   MASS SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Required for respiration and the maintenance of the
CC       mitochondrial compartment. May have a role in the mitochondrial
CC       synthesis of fatty acids.
CC   -!- CATALYTIC ACTIVITY: Acyl-[acyl-carrier-protein] + NADP(+) = trans-
CC       2,3-dehydroacyl-[acyl-carrier-protein] + NADPH.
CC   -!- CATALYTIC ACTIVITY: Acyl-CoA + NADP(+) = trans-2,3-dehydroacyl-CoA
CC       + NADPH.
CC   -!- SUBUNIT: Homodimer or in a complex with other proteins. Interacts
CC       with ARS1.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC   -!- MISCELLANEOUS: Present with 1560 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family. Quinone oxidoreductase subfamily.
CC   -!- CAUTION: Was originally (PubMed:8195160) thought to be a nuclear
CC       protein involved in transcriptional regulation of genes required
CC       for the functional assembly of mitochondrial respiratory proteins.
CC       This was later proven not to be the case (PubMed:11509667).
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DR   EMBL; D26606; BAA05651.1; -; Genomic_DNA.
DR   EMBL; Z35895; CAA84968.1; -; Genomic_DNA.
DR   EMBL; X76078; CAA53683.1; -; Genomic_DNA.
DR   EMBL; AY557872; AAS56198.1; -; Genomic_DNA.
DR   RefSeq; NP_009582.1; -.
DR   HSSP; Q8WZM3; 1GYR.
DR   IntAct; P38071; -.
DR   PeptideAtlas; P38071; -.
DR   Ensembl; YBR026C; Saccharomyces cerevisiae.
DR   GeneID; 852314; -.
DR   GenomeReviews; Y13134_GR; YBR026C.
DR   KEGG; sce:YBR026C; -.
DR   NMPDR; fig|4932.3.peg.277; -.
DR   CYGD; YBR026c; -.
DR   SGD; S000000230; ETR1.
DR   HOGENOM; P38071; -.
DR   LinkHub; P38071; -.
DR   NextBio; 971002; -.
DR   GermOnline; YBR026C; Saccharomyces cerevisiae.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004319; F:enoyl-[acyl-carrier-protein] reductase (NAD...; IEA:EC.
DR   GO; GO:0019166; F:trans-2-enoyl-CoA reductase (NADPH) activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0009060; P:aerobic respiration; IMP:SGD.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013154; AlcDHase_GroES-like.
DR   InterPro; IPR002085; AlcDHase_SF_Zn.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11695; ADH_Sf_Zn; 1.
DR   Pfam; PF08240; ADH_N; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Direct protein sequencing; DNA-binding;
KW   Fatty acid biosynthesis; Lipid synthesis; Mitochondrion; NADP;
KW   Oxidoreductase; Phosphoprotein; Transit peptide.
FT   TRANSIT       1      9       Mitochondrion.
FT   CHAIN        10    380       Enoyl-[acyl-carrier protein] reductase
FT                                [NADPH, B-specific], mitochondrial.
FT                                /FTId=PRO_0000160924.
FT   MOD_RES     135    135       Phosphoserine.
FT   MOD_RES     339    339       Phosphoserine.
FT   MUTAGEN      73     73       Y->N: 0.1% of catalytic activity. No
FT                                specific ARS1 binding.
FT   CONFLICT     24     24       I -> T (in Ref. 4; AAS56198).
SQ   SEQUENCE   380 AA;  42067 MW;  9795013283C3E9F8 CRC64;
     MLPTFKRYMS SSAHQIPKHF KSLIYSTHEV EDCTKVLSVK NYTPKQDLSQ SIVLKTLAFP
     INPSDINQLQ GVYPSRPEKT YDYSTDEPAA IAGNEGVFEV VSLPSGSSKG DLKLGDRVIP
     LQANQGTWSN YRVFSSSSDL IKVNDLDLFS AATVSVNGCT GFQLVSDYID WNSNGNEWII
     QNAGTSSVSK IVTQVAKAKG IKTLSVIRDR DNFDEVAKVL EDKYGATKVI SESQNNDKTF
     AKEVLSKILG ENARVRLALN SVGGKSSASI ARKLENNALM LTYGGMSKQP VTLPTSLHIF
     KGLTSKGYWV TEKNKKNPQS KIDTISDFIK MYNYGHIISP RDEIETLTWN TNTTTDEQLL
     ELVKKGITGK GKKKMVVLEW
//