ID   NIR_BETVE               Reviewed;         583 AA.
AC   P38500;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-NOV-2008, entry version 64.
DE   RecName: Full=Ferredoxin--nitrite reductase, chloroplastic;
DE            EC=1.7.7.1;
DE   Flags: Precursor;
GN   Name=NIR1;
OS   Betula verrucosa (White birch) (Betula pendula).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fagales; Betulaceae; Betula.
OX   NCBI_TaxID=3505;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   MEDLINE=92167960; PubMed=1347145; DOI=10.1007/BF00292710;
RA   Friemann A., Brinkmann K., Hachtel W.;
RT   "Sequence of a cDNA encoding nitrite reductase from the tree Betula
RT   pendula and identification of conserved protein regions.";
RL   Mol. Gen. Genet. 231:411-416(1992).
CC   -!- CATALYTIC ACTIVITY: NH(3) + 2 H(2)O + 6 oxidized ferredoxin =
CC       nitrite + 6 reduced ferredoxin + 7 H(+).
CC   -!- COFACTOR: Binds 1 siroheme per subunit.
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
CC   -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast (By similarity).
CC   -!- INDUCTION: By nitrate.
CC   -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S
CC       domain family.
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DR   EMBL; X60093; CAA42690.1; -; mRNA.
DR   PIR; S20495; S20495.
DR   SMR; P38500; 42-576.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-KW.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0048307; F:ferredoxin-nitrite reductase activity; IEA:EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0006810; P:transport; IEA:UniProtKB-KW.
DR   InterPro; IPR006066; Nir_Si_BS.
DR   InterPro; IPR006067; Nir_Sir_4Fe4S.
DR   InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR   Pfam; PF01077; NIR_SIR; 1.
DR   Pfam; PF03460; NIR_SIR_ferr; 2.
DR   PRINTS; PR00397; SIROHAEM.
DR   PROSITE; PS00365; NIR_SIR; 1.
PE   2: Evidence at transcript level;
KW   4Fe-4S; Chloroplast; Electron transport; Heme; Iron; Iron-sulfur;
KW   Metal-binding; Nitrate assimilation; Oxidoreductase; Plastid;
KW   Transit peptide; Transport.
FT   TRANSIT       1     22       Chloroplast (Potential).
FT   CHAIN        23    583       Ferredoxin--nitrite reductase,
FT                                chloroplastic.
FT                                /FTId=PRO_0000019704.
FT   METAL       461    461       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       467    467       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       502    502       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       506    506       Iron (siroheme axial ligand) (By
FT                                similarity).
FT   METAL       506    506       Iron-sulfur (4Fe-4S) (By similarity).
SQ   SEQUENCE   583 AA;  65229 MW;  482111EF7E66991F CRC64;
     MSSLSVRFLS PPLFSSTPAW PRTGLAATQA VPPVVAEVDA GRLEPRVEER EGYWVLKEKF
     REGINPQEKL KLEREPMKLF MEGGIEDLAK MSLEEIDKDK ISKSDIDVRL KWLGLFHRRK
     HHYGRFMMRL KLPNGVTTSA QTRYLASVIR KYGKDGCADV TTRQNWQIRG VVLSDVPEIL
     KGLDEVGLTS LQSGMDNVRN PVGNPLAGID IHEIVATRPY NNLLSQFITA NSRGNLAFTN
     LPRKWNVCVV GSHDLFEHPH INDLAYMPAI KDGRFGFNLL VGGFFSPRRC AEAVPLDAWV
     SADDIILVCK AILEAYRDLG TRGNRQKTRM MWLIDELGIE GFRSEVVKRM PNQELERAAP
     EDLIEKQWER RELIGVHPQK QEGLSYVGLH IPVGRVQADD MDELARLADT YGCGELRLTV
     EQNIIIPNIE NSKLEALLGE PLLKDRFSPE PPILMKGLVA CTGNQFCGQA IIETKARALK
     VTEEVQRQVA VTRPVRMHWT GCPNSCGQVQ VADIGFMGCM ARDENGKPCE GAAVFLGGRI
     GSDSHLGNLY KKGVPCKNLV PLVVDILVKH FGAVPREREE SED
//