ID   DHE5_YEAST              Reviewed;         457 AA.
AC   P39708;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-NOV-2008, entry version 59.
DE   RecName: Full=NADP-specific glutamate dehydrogenase 2;
DE            Short=NADP-GDH 2;
DE            EC=1.4.1.4;
DE   AltName: Full=NADP-dependent glutamate dehydrogenase 2;
GN   Name=GDH3; OrderedLocusNames=YAL062W; ORFNames=FUN51;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   MEDLINE=95249563; PubMed=7731988;
RA   Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N.,
RA   Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J.,
RA   Storms R.K.;
RT   "The nucleotide sequence of chromosome I from Saccharomyces
RT   cerevisiae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995).
CC   -!- CATALYTIC ACTIVITY: L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate
CC       + NH(3) + NADPH.
CC   -!- SUBUNIT: Homohexamer (By similarity).
CC   -!- INTERACTION:
CC       Q06150:BOP2; NbExp=1; IntAct=EBI-5832, EBI-34293;
CC   -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
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DR   EMBL; U12980; AAC04972.1; -; Genomic_DNA.
DR   PIR; S51960; S51960.
DR   RefSeq; NP_009339.1; -.
DR   HSSP; P24295; 1AUP.
DR   DIP; DIP:4325N; -.
DR   IntAct; P39708; -.
DR   PeptideAtlas; P39708; -.
DR   Ensembl; YAL062W; Saccharomyces cerevisiae.
DR   GeneID; 851237; -.
DR   GenomeReviews; U00091_GR; YAL062W.
DR   KEGG; sce:YAL062W; -.
DR   NMPDR; fig|4932.3.peg.13; -.
DR   CYGD; YAL062w; -.
DR   SGD; S000000058; GDH3.
DR   HOGENOM; P39708; -.
DR   BioCyc; MetaCyc:MON-11505; -.
DR   LinkHub; P39708; -.
DR   NextBio; 968159; -.
DR   ArrayExpress; P39708; -.
DR   GermOnline; YAL062W; Saccharomyces cerevisiae.
DR   GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0005625; C:soluble fraction; IDA:SGD.
DR   GO; GO:0004354; F:glutamate dehydrogenase (NADP+) activity; IEA:EC.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0019676; P:ammonia assimilation cycle; IEP:SGD.
DR   GO; GO:0006537; P:glutamate biosynthetic process; IGI:SGD.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006095; Glu/Leu/Phe/Val_DHase.
DR   InterPro; IPR006096; Glu/Leu/Phe/Val_DHase_C.
DR   InterPro; IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
DR   InterPro; IPR014362; Glu_DHase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11606:SF2; GLFV_DH; 1.
DR   Pfam; PF00208; ELFV_dehydrog; 1.
DR   Pfam; PF02812; ELFV_dehydrog_N; 1.
DR   PIRSF; PIRSF000185; Glu_DH; 1.
DR   PRINTS; PR00082; GLFDHDRGNASE.
DR   PROSITE; PS00074; GLFV_DEHYDROGENASE; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; NADP; Oxidoreductase.
FT   CHAIN         1    457       NADP-specific glutamate dehydrogenase 2.
FT                                /FTId=PRO_0000182799.
FT   NP_BIND     175    204       NAD (By similarity).
FT   ACT_SITE    111    111       By similarity.
SQ   SEQUENCE   457 AA;  49627 MW;  81B30625038B1888 CRC64;
     MTSEPEFQQA YDEIVSSVED SKIFEKFPQY KKVLPIVSVP ERIIQFRVTW ENDNGEQEVA
     QGYRVQFNSA KGPYKGGLRF HPSVNLSILK FLGFEQIFKN ALTGLDMGGG KGGLCVDLKG
     KSDNEIRRIC YAFMRELSRH IGKDTDVPAG DIGVGGREIG YLFGAYRSYK NSWEGVLTGK
     GLNWGGSLIR PEATGFGLVY YTQAMIDYAT NGKESFEGKR VTISGSGNVA QYAALKVIEL
     GGIVVSLSDS KGCIISETGI TSEQIHDIAS AKIRFKSLEE IVDEYSTFSE SKMKYVAGAR
     PWTHVSNVDI ALPCATQNEV SGDEAKALVA SGVKFVAEGA NMGSTPEAIS VFETARSTAT
     NAKDAVWFGP PKAANLGGVA VSGLEMAQNS QKVTWTAERV DQELKKIMIN CFNDCIQAAQ
     EYSTEKNTNT LPSLVKGANI ASFVMVADAM LDQGDVF
//