ID   BDH1_YEAST              Reviewed;         382 AA.
AC   P39714;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-NOV-2008, entry version 70.
DE   RecName: Full=(R,R)-butanediol dehydrogenase;
DE            EC=1.1.1.4;
GN   Name=BDH1; Synonyms=BDH; OrderedLocusNames=YAL060W; ORFNames=FUN49;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   MEDLINE=95249563; PubMed=7731988;
RA   Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N.,
RA   Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J.,
RA   Storms R.K.;
RT   "The nucleotide sequence of chromosome I from Saccharomyces
RT   cerevisiae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995).
RN   [2]
RP   CHARACTERIZATION.
RC   STRAIN=ATCC 90845 / FY834;
RX   MEDLINE=20549593; PubMed=10938079; DOI=10.1074/jbc.M003035200;
RA   Gonzalez E., Fernandez M.R., Larroy C., Sola L., Pericas M.A.,
RA   Pares X., Biosca J.A.;
RT   "Characterization of a (2R,3R)-2,3-butanediol dehydrogenase as the
RT   Saccharomyces cerevisiae YAL060W gene product. Disruption and
RT   induction of the gene.";
RL   J. Biol. Chem. 275:35876-35885(2000).
RN   [3]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- CATALYTIC ACTIVITY: (R,R)-butane-2,3-diol + NAD(+) = (R)-acetoin +
CC       NADH.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- SUBUNIT: Homodimer.
CC   -!- INTERACTION:
CC       P40054:SER3; NbExp=1; IntAct=EBI-3501, EBI-16961;
CC   -!- MISCELLANEOUS: Present with 8730 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family.
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DR   EMBL; U12980; AAC04974.1; -; Genomic_DNA.
DR   PIR; S51962; S51962.
DR   RefSeq; NP_009341.1; -.
DR   HSSP; O96496; 1E3J.
DR   DIP; DIP:5356N; -.
DR   IntAct; P39714; -.
DR   PeptideAtlas; P39714; -.
DR   Ensembl; YAL060W; Saccharomyces cerevisiae.
DR   GeneID; 851239; -.
DR   GenomeReviews; U00091_GR; YAL060W.
DR   KEGG; sce:YAL060W; -.
DR   NMPDR; fig|4932.3.peg.15; -.
DR   CYGD; YAL060w; -.
DR   SGD; S000000056; BDH1.
DR   HOGENOM; P39714; -.
DR   BioCyc; MetaCyc:MON-14023; -.
DR   LinkHub; P39714; -.
DR   NextBio; 968165; -.
DR   GermOnline; YAL060W; Saccharomyces cerevisiae.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0000721; F:(R,R)-butanediol dehydrogenase activity; IEA:EC.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006066; P:cellular alcohol metabolic process; IMP:SGD.
DR   GO; GO:0055114; P:oxidation reduction; IEA:InterPro.
DR   InterPro; IPR013154; AlcDHase_GroES-like.
DR   InterPro; IPR002085; AlcDHase_SF_Zn.
DR   InterPro; IPR013149; AlcDHase_Zn-bd.
DR   InterPro; IPR002328; AlcDHase_Zn_CS.
DR   PANTHER; PTHR11695; ADH_Sf_Zn; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase; Zinc.
FT   CHAIN         1    382       (R,R)-butanediol dehydrogenase.
FT                                /FTId=PRO_0000160788.
FT   METAL        39     39       Zinc 1; catalytic (By similarity).
FT   METAL        73     73       Zinc 1; catalytic (By similarity).
FT   METAL       103    103       Zinc 2 (By similarity).
FT   METAL       120    120       Zinc 2 (By similarity).
FT   METAL       123    123       Zinc 2 (By similarity).
FT   METAL       131    131       Zinc 2 (By similarity).
FT   METAL       173    173       Zinc 1; catalytic (By similarity).
SQ   SEQUENCE   382 AA;  41538 MW;  1006FA596DE91A17 CRC64;
     MRALAYFKKG DIHFTNDIPR PEIQTDDEVI IDVSWCGICG SDLHEYLDGP IFMPKDGECH
     KLSNAALPLA MGHEMSGIVS KVGPKVTKVK VGDHVVVDAA SSCADLHCWP HSKFYNSKPC
     DACQRGSENL CTHAGFVGLG VISGGFAEQV VVSQHHIIPV PKEIPLDVAA LVEPLSVTWH
     AVKISGFKKG SSALVLGAGP IGLCTILVLK GMGASKIVVS EIAERRIEMA KKLGVEVFNP
     SKHGHKSIEI LRGLTKSHDG FDYSYDCSGI QVTFETSLKA LTFKGTATNI AVWGPKPVPF
     QPMDVTLQEK VMTGSIGYVV EAFEEVVRAI HNGDIAMEDC KQLITGKQRI EDGWEKGFQE
     LMDHKESNVK ILLTPNNHGE MK
//