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UniProtKB/Swiss-Prot entry P39863

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NIA_FUSOX
Primary accession number P39863
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1995
Sequence was last modified on February 1, 1995 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 62)
Name and origin of the protein
Protein name Nitrate reductase [NADPH]
Synonyms NR
EC 1.7.1.3
Gene name
Name: NIA
From
Fusarium oxysporum [TaxID: 5507] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Hypocreales; mitosporic Hypocreales; Fusarium; Fusarium oxysporum species complex.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=FOM24;
DOI=10.1016/0378-1119(93)90669-T; PubMed=8370541 [NCBI, ExPASy, EBI, Israel, Japan]
Diolez A., Langin T., Gerlinger C., Brygoo Y., Daboussi M.-J.;
"The nia gene of Fusarium oxysporum: isolation, sequence and development of a homologous transformation system.";
Gene 131:61-67(1993).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z22549; CAA80270.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JN0803; JN0803.
3D structure databases
HSSP P04166; 1EUE. [HSSP ENTRY / PDB]
ModBase P39863.
Ontologies
GO
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0050660; Molecular function: FAD binding (inferred from electronic annotation from InterPro).
GO:0020037; Molecular function: heme binding (inferred from electronic annotation from InterPro).
GO:0005506; Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0030151; Molecular function: molybdenum ion binding (inferred from electronic annotation from InterPro).
GO:0050464; Molecular function: nitrate reductase (NADPH) activity (inferred from electronic annotation from EC).
GO:0042128; Biological process: nitrate assimilation (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001199; Cyt_B5.
IPR001834; Cyt_B5_reductase.
IPR001709; FPN_cyt_redctse.
IPR005066; MoCF_OxRdtse_dimer.
IPR008335; Mopterin_OxRdtase_euk.
IPR012137; Nitr_rd_NADH.
IPR008333; OxRdtase_FAD-bd.
IPR001433; OxRdtase_FAD/NAD_bd.
IPR000572; OxRdtase_Mopterin-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.10.120.10; Cyt_B5; 1.
G3DSA:2.60.40.650; MoCF_oxrdtse_dimer; 1.
G3DSA:3.90.420.10; Oxred_molyb_bd; 1.
Pfam PF00173; Cyt-b5; 1.
PF00970; FAD_binding_6; 1.
PF03404; Mo-co_dimer; 1.
PF00175; NAD_binding_1; 1.
PF00174; Oxidored_molyb; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000233; Nitr_rd_NADH; 1.
PRINTS PR00406; CYTB5RDTASE.
PR00363; CYTOCHROMEB5.
PR00407; EUMOPTERIN.
PR00371; FPNCR.
ProDom PD000612; Cyt_B5; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00191; CYTOCHROME_B5_1; 1.
PS50255; CYTOCHROME_B5_2; 1.
PS51384; FAD_FR; 1.
PS00559; MOLYBDOPTERIN_EUK; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P39863.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
FAD; Flavoprotein; Heme; Iron; Metal-binding; Molybdenum; NADP; Nitrate assimilation; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   905  905     Nitrate reductase [NADPH]. PRO_0000166043
DOMAIN   546   621  76     Cytochrome b5 heme-binding. 
DOMAIN   648   759  112     FAD-binding FR-type. 
NP_BIND   875   884  10     NADP (By similarity). 
COMPBIAS   3     8  6     Poly-Thr. 
METAL   179   179        Molybdenum-pterin (Potential). 
METAL   230   230        Molybdenum-pterin (Potential). 
METAL   581   581        Iron (heme axial ligand) (By similarity). 
METAL   604   604        Iron (heme axial ligand) (By similarity). 
DISULFID   428   428        Interchain (Potential). 
Sequence information
Length: 905 AA [This is the length of the unprocessed precursor] Molecular weight: 101899 Da [This is the MW of the unprocessed precursor] CRC64: D5D8E23F7971ACDA [This is a checksum on the sequence] 
        10         20         30         40         50         60 
METSTTTTLL QQERIPENSE PISTHIHTHS LPPTPPGTAK PSRIGSFHDL QELSSVESPR 

        70         80         90        100        110        120 
LDHIKPYPLP PKFSPKSVLK EDLKTPDNFV ERDPRLIRLT GVHPFNVEAP LSDLYDEGFL 

       130        140        150        160        170        180 
TSENLHYVRN HGHVPRCEDD DILDWEFEIS GLVENPIKMN VRDLINDYQQ LTYPVTFVCA 

       190        200        210        220        230        240 
GNRRKEQNIV RKTKGFSWGP AGLSTALWTG VAIGDLLSAA KPKRGARYVC FEGADKLPNG 

       250        260        270        280        290        300 
YYGTSIKLNW CMDPNRGVMV AHKMNGNPLP PDHGKPVRIV IPGQIGGRSI KWLKKITITQ 

       310        320        330        340        350        360 
EPSDNWYHIY DNRVLPTMIS PEESANLPEV WKDEKYAIYD LSTNSAICYP AHEEKVPFTD 

       370        380        390        400        410        420 
APASYKVRGY AYSGGGRRIT RVEVTLDKGK SWRLANIRYP EDDYRNAPEG DTLYGGRVDM 

       430        440        450        460        470        480 
WWRETSFCWC FWDLDIPLDE LKSADDIMMR AMDESMNVQP RDMYWSVLGM MNNPWFRIVI 

       490        500        510        520        530        540 
HKEDHALRFE HPTHATLKIK GWMERVKEAG GDLTNGYWGE KAPGEVQEVV VKEPEKQICM 

       550        560        570        580        590        600 
TNPQINRKIT IEELKAHSGE EEPWFVVKGE VYDGTPYLSG HPGGAASIFG AAGQDATEEF 

       610        620        630        640        650        660 
MAIHSENAKA MLPTYHIGTL DEESRAILSG DATKTNDDAD REVFLQAKTW SKAILDKKTS 

       670        680        690        700        710        720 
ISPDTKIFSF KLNHEAQKIG LPTGQHLMMR LRDPATREAI IRSYTPYSDG SDCGRLDILI 

       730        740        750        760        770        780 
KIYYDTPQRK GGVMTQALDA LPIGHWVDFK GPTGKFVYHG NGLCTINEKE RRVRRFIMVC 

       790        800        810        820        830        840 
GGSGITPIRQ VLRAVIDNPK DTTPCLVFNG NRSVNDILCM EELEELEAAN PSRCRVVNAL 

       850        860        870        880        890        900 
SNPPPEWNGL KGFVNQALVP EYMDLPKASG EGDELLLVCG PPPMVKAVEA SFLGMGFKSD 


DFVFF
P39863 in FASTA format

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