[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Leaf; Wu S., Lu Q., Kriz A.L., Harper J.E.; "Molecular cloning and analysis winged bean and soybean bispecific constitutive NR and their NiR."; Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
CATALYTIC ACTIVITY: Nitrite + NAD⁺ + H₂O = nitrate + NADH.
COFACTOR: Binds 1 FAD.
COFACTOR: Binds 1 heme group. The heme group is called cytochrome b-557.
COFACTOR: Binds 1 molybdenum ion.
SUBUNIT: Homodimer (By similarity).
SIMILARITY: Belongs to the nitrate reductase family.
SIMILARITY: Contains 1 cytochrome b5 heme-binding domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L16780; AAA80564.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P17571; 2CND. [HSSP ENTRY / PDB]

ModBase

P39882.

Family and domain databases

InterPro

IPR001199; Cyt_B5.
IPR001834; Cyt_B5_reductase.
IPR001709; FPN_cyt_redctse.
IPR001433; OxRdtase_FAD/NAD_bd.
IPR000572; OxRdtase_Mopterin-bd.
Graphical view of domain structure.

Pfam

PF00175; NAD_binding_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00406; CYTB5RDTASE.
PR00371; FPNCR.

PROSITE

PS00191; CYTOCHROME_B5_1; PARTIAL.
PS50255; CYTOCHROME_B5_2; PARTIAL.
PS00559; MOLYBDOPTERIN_EUK; PARTIAL.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

FAD; Flavoprotein; Heme; Iron; Metal-binding; Molybdenum; NAD; Nitrate assimilation; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 160`	`>160`	`Nitrate reductase [NADH].`	`PRO_0000166060`
`NON_TER`	`1 1`

Sequence information

Length: 160 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 17728 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: D6CFC1447393A932 [This is a checksum on the sequence]

        10         20         30         40         50         60 
KGPLGHIEYT GKGNFIAHGE PKFARRLAML AGGTGITPIY QVIQAVLKDP DDRTEMFFAV 

        70         80         90        100        110        120 
YSNKTESDIL LREELDAWAK KHSDRFKVWY VVDKAGNDWA FSTGRVNESI MRVHLPGPSD 

       130        140        150        160 
ALALACGPPP INSAYGWQPS LENIGYKKDD VPVFMATTQS

P39882 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools