ID   NIA_LOTTE               Reviewed;         160 AA.
AC   P39882;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-NOV-2008, entry version 50.
DE   RecName: Full=Nitrate reductase [NADH];
DE            Short=NR;
DE            EC=1.7.1.1;
DE   Flags: Fragment;
GN   Name=NIA;
OS   Lotus tetragonolobus (Winged pea) (Tetragonolobus purpureus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Loteae; Lotus.
OX   NCBI_TaxID=3868;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RA   Wu S., Lu Q., Kriz A.L., Harper J.E.;
RT   "Molecular cloning and analysis winged bean and soybean bispecific
RT   constitutive NR and their NiR.";
RL   Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Nitrate reductase is a key enzyme involved in the first
CC       step of nitrate assimilation in plants, fungi and bacteria.
CC   -!- CATALYTIC ACTIVITY: Nitrite + NAD(+) + H(2)O = nitrate + NADH.
CC   -!- COFACTOR: Binds 1 FAD.
CC   -!- COFACTOR: Binds 1 heme group. The heme group is called cytochrome
CC       b-557.
CC   -!- COFACTOR: Binds 1 molybdenum ion.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the nitrate reductase family.
CC   -!- SIMILARITY: Contains 1 cytochrome b5 heme-binding domain.
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DR   EMBL; L16780; AAA80564.1; -; mRNA.
DR   HSSP; P17571; 2CND.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030151; F:molybdenum ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009703; F:nitrate reductase (NADH) activity; IEA:EC.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001199; Cyt_B5.
DR   InterPro; IPR001834; Cyt_B5_reductase.
DR   InterPro; IPR001709; FPN_cyt_redctse.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD_bd.
DR   InterPro; IPR000572; OxRdtase_Mopterin-bd.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00406; CYTB5RDTASE.
DR   PRINTS; PR00371; FPNCR.
DR   PROSITE; PS00191; CYTOCHROME_B5_1; PARTIAL.
DR   PROSITE; PS50255; CYTOCHROME_B5_2; PARTIAL.
DR   PROSITE; PS00559; MOLYBDOPTERIN_EUK; PARTIAL.
PE   2: Evidence at transcript level;
KW   FAD; Flavoprotein; Heme; Iron; Metal-binding; Molybdenum; NAD;
KW   Nitrate assimilation; Oxidoreductase.
FT   CHAIN        <1    160       Nitrate reductase [NADH].
FT                                /FTId=PRO_0000166060.
FT   NON_TER       1      1
SQ   SEQUENCE   160 AA;  17728 MW;  D6CFC1447393A932 CRC64;
     KGPLGHIEYT GKGNFIAHGE PKFARRLAML AGGTGITPIY QVIQAVLKDP DDRTEMFFAV
     YSNKTESDIL LREELDAWAK KHSDRFKVWY VVDKAGNDWA FSTGRVNESI MRVHLPGPSD
     ALALACGPPP INSAYGWQPS LENIGYKKDD VPVFMATTQS
//