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UniProtKB/Swiss-Prot entry P40288

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHG_BACME
Primary accession number P40288
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1995
Sequence was last modified on February 1, 1995 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 50)
Name and origin of the protein
Protein name Glucose 1-dehydrogenase
Synonym EC 1.1.1.47
Gene name None
From
Bacillus megaterium [TaxID: 1404] 
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-29, AND MUTAGENESIS.
STRAIN=IWG3;
PubMed=2495285 [NCBI, ExPASy, EBI, Israel, Japan]
Makino Y., Negoro S., Urabe I., Okada H.;
"Stability-increasing mutants of glucose dehydrogenase from Bacillus megaterium IWG3.";
J. Biol. Chem. 264:6381-6385(1989).
[2]
 SEQUENCE REVISION.
Urabe I.;
Submitted (MAR-1989) to the EMBL/GenBank/DDBJ databases.
[3]
 X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) IN COMPLEX WITH NAD.
PubMed=11173533 [NCBI, ExPASy, EBI, Israel, Japan]
Yamamoto K., Kurisu G., Kusunoki M., Tabata S., Urabe I., Osaki S.;
"Crystal structure of glucose dehydrogenase from Bacillus megaterium IWG3 at 1.7 A resolution.";
J. Biochem. 129:303-312(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
J04805; AAA22475.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A33528; A33528.
3D structure databases
PDB
1G6K; X-ray; 2.00 A; A/B/E/F=1-261.[ExPASy / RCSB / EBI]
1GCO; X-ray; 1.70 A; A/B/E/F=1-261.[ExPASy / RCSB / EBI]
1GEE; X-ray; 1.60 A; A/B/E/F=1-261.[ExPASy / RCSB / EBI]
1RWB; X-ray; 2.00 A; A/B/E/F=1-261.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1G6K; -.
1GCO; -.
1GEE; -.
1RWB; -.
ModBase P40288.
Family and domain databases
InterPro IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR19410; ADH_short_C2; 1.
Pfam PF00106; adh_short; 1.
Pfam graphical view of domain structure.
PRINTS PR00081; GDHRDH.
PR00080; SDRFAMILY.
PROSITE PS00061; ADH_SHORT; 1.
BLOCKS P40288.
Other
ProtoNet P40288.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Direct protein sequencing; NADP; Oxidoreductase; Sporulation.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   261  261     Glucose 1-dehydrogenase. PRO_0000054615
NP_BIND   11    35  25     NADP. 
ACT_SITE   158   158        Proton acceptor. 
BINDING   145   145        Substrate (By similarity). 
MUTAGEN   96    96        E->A,G,K: Heat stable. 
MUTAGEN   108   108        D->N: Heat stable. 
MUTAGEN   112   112        V->A: Heat stable. 
MUTAGEN   133   133        E->K: Heat stable. 
MUTAGEN   183   183        V->I: Heat stable. 
MUTAGEN   194   194        P->Q: Heat stable. 
MUTAGEN   210   210        E->K: Heat stable. 
MUTAGEN   217   217        Y->H: Heat stable. 
MUTAGEN   252   252        Q->L: Heat stable. 
MUTAGEN   253   253        Y->C: Heat stable. 
MUTAGEN   258   258        A->G: Heat stable. 
HELIX   3     5  3      
STRAND   9    12  4      
HELIX   18    29  12      
STRAND   33    40  8      
HELIX   42    54  13      
STRAND   58    63  6      
HELIX   69    83  15      
STRAND   88    91  4      
HELIX   101   103  3      
HELIX   106   116  11      
HELIX   118   133  16      
STRAND   139   143  5      
HELIX   146   148  3      
HELIX   156   176  21      
HELIX   177   179  3      
STRAND   182   188  7      
HELIX   194   196  3      
TURN   197   201  5      
HELIX   203   210  8      
HELIX   221   232  12      
HELIX   234   236  3      
STRAND   243   247  5      
HELIX   250   252  3      
HELIX   254   259  6      
Sequence information
Length: 261 AA [This is the length of the unprocessed precursor] Molecular weight: 28085 Da [This is the MW of the unprocessed precursor] CRC64: C23AC98D304EEB2F [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MYKDLEGKVV VITGSSTGLG KSMAIRFATE KAKVVVNYRS KEDEANSVLE EIKKVGGEAI 

        70         80         90        100        110        120 
AVKGDVTVES DVINLVQSAI KEFGKLDVMI NNAGLENPVS SHEMSLSDWN KVIDTNLTGA 

       130        140        150        160        170        180 
FLGSREAIKY FVENDIKGTV INMSSVHEKI PWPLFVHYAA SKGGMKLMTE TLALEYAPKG 

       190        200        210        220        230        240 
IRVNNIGPGA INTPINAEKF ADPEQRADVE SMIPMGYIGE PEEIAAVAAW LASSEASYVT 

       250        260 
GITLFADGGM TQYPSFQAGR G
P40288 in FASTA format

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