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UniProtKB/Swiss-Prot entry P40375


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAOX_SCHPO
Primary accession number P40375
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1995
Sequence was last modified on February 1, 1995 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 61)
Name and origin of the protein
Protein name NAD-dependent malic enzyme
Synonyms NAD-ME
EC 1.1.1.38
Gene name
Name: mae2
ORFNames: SPCC794.12c
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
PubMed=7941746 [NCBI, ExPASy, EBI, Israel, Japan]
Viljoen M., Subden R.E., Krizus A., van Vuuren H.J.J.;
"Molecular analysis of the malic enzyme gene (mae2) of Schizosaccharomyces pombe.";
Yeast 10:613-624(1994).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
[3]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-445, AND MASS SPECTROMETRY.
DOI=10.1021/pr7006335; PubMed=18257517 [NCBI, ExPASy, EBI, Israel, Japan]
Wilson-Grady J.T., Villen J., Gygi S.P.;
"Phosphoproteome analysis of fission yeast.";
J. Proteome Res. 7:1088-1097(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U00621; AAA18985.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CU329672; CAA19139.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S44330; S44330.
RefSeq NP_587760.1; -.
3D structure databases
HSSP P23368; 1GZ3. [HSSP ENTRY / PDB]
ModBase P40375.
Enzyme and pathway databases
BioCyc SPOM-XXX-01:SPOM-XXX-01-002119-MON; -.
Organism-specific databases
GeneDB_Spombe SPCC794.12c; -.
Gene expression databases
ArrayExpress P40375; -.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from direct assay from GeneDB_SPombe).
GO:0016619; Molecular function: malate dehydrogenase (oxaloacetate-decarboxylating) activity (inferred from electronic annotation from EC).
GO:0046872; Molecular function: metal ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051287; Molecular function: NAD binding (inferred from electronic annotation from InterPro).
GO:0006108; Biological process: malate metabolic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006090; Biological process: pyruvate metabolic process (inferred from mutant phenotype from GeneDB_SPombe).
QuickGo view.
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
ProtoNet P40375.
Genome annotation databases
GeneID 2539592; -.
KEGG spo:SPCC794.12c; -.
NMPDR fig|4896.1.peg.98; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Metal-binding; NAD; Oxidoreductase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   565  565     NAD-dependent malic enzyme. PRO_0000160205
ACT_SITE   103   103        Proton donor (By similarity). 
ACT_SITE   177   177        Proton acceptor (By similarity). 
METAL   248   248        Divalent metal cation (By similarity). 
METAL   249   249        Divalent metal cation (By similarity). 
METAL   272   272        Divalent metal cation (By similarity). 
BINDING   272   272        NAD (By similarity). 
BINDING   419   419        NAD (By similarity). 
SITE   272   272  1     Important for activity (By similarity). 
MOD_RES   445   445        Phosphoserine. 
Sequence information
Length: 565 AA [This is the length of the unprocessed precursor] Molecular weight: 62535 Da [This is the MW of the unprocessed precursor] CRC64: AF22D98E1BB72CA8 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPAGTKEQIE CPLKGVTLLN SPRYNKDTAF TPEERQKFEI SSRLPPIVET LQQQVDRCYD 

        70         80         90        100        110        120 
QYKAIGDEPL QKNLYLSQLS VTNQTLFYAL ISQHLIEMIP IIYTPTEGDA IKQFSDIYRY 

       130        140        150        160        170        180 
PEGCYLDIDH NDLSYIKQQL SEFGKSDSVE YIIITDSEGI LGIGDQGVGG VLISVAKGHL 

       190        200        210        220        230        240 
MTLCAGLDPN RFLPIVLDVG TNNETHRKNH QYMGLRKDRV RGEQYDSFLD NVIKAIREVF 

       250        260        270        280        290        300 
PEAFIHFEDF GLANAKRILD HYRPDIACFN DDIQGTGAVA LAAIIGALHV TKSPLTEQRI 

       310        320        330        340        350        360 
MIFGAGTAGV GIANQIVAGM VTDGLSLDKA RGNLFMIDRC GLLLERHAKI ATDGQKPFLK 

       370        380        390        400        410        420 
KDSDFKEVPS GDINLESAIA LVKPTILLGC SGQPGKFTEK AIREMSKHVE RPIIFPISNP 

       430        440        450        460        470        480 
TTLMEAKPDQ IDKWSDGKAL IATGSPLPPL NRNGKKYVIS QCNNALLYPA LGVACVLSRC 

       490        500        510        520        530        540 
KLLSDGMLKA ASDALATVPR SLFAADEALL PDLNNAREIS RHIVFAVLKQ AVSEGMSTVD 

       550        560 
LPKDDAKLKE WIIEREWNPE YKPFV 

P40375 in FASTA format

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