NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-31; 122-143; 230-259 AND 268-283.
PubMed=1368326 [NCBI, ExPASy, EBI, Israel, Japan]
Suzuki K., Ogishima M., Sugiyama M., Inouye Y., Nakamura S., Imamura S.;
"Molecular cloning and expression of a Streptomyces sarcosine oxidase gene in Streptomyces lividans.";
Biosci. Biotechnol. Biochem. 56:432-436(1992).

Comments

FUNCTION: Catalyzes the oxidative demethylation of sarcosine (By similarity).
CATALYTIC ACTIVITY: Sarcosine + H₂O + O₂ = glycine + formaldehyde + H₂O₂.
COFACTOR: Binds 1 FAD per subunit (By similarity).
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the MSOX/MTOX family. MSOX subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

S39464; AAC60412.2; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D10623; BAA01473.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P40859; 1EL5. [HSSP ENTRY / PDB]

ModBase

P40854.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0008115;	Molecular function: sarcosine oxidase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00516; -; 1.
PBIL [Tree]

InterPro

IPR006076; FAD-dep_OxRdtase.
Graphical view of domain structure.

Pfam

PF01266; DAO; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 389`	`388`	`Monomeric sarcosine oxidase.`	`PRO_0000213764`
`NP_BIND`	`6 36`	`31`	`FAD (Potential).`
`MOD_RES`	`315 315`		`S-8alpha-FAD cysteine (By similarity).`

Sequence information

Length: 389 AA [This is the length of the unprocessed precursor]

Molecular weight: 42263 Da [This is the MW of the unprocessed precursor]

CRC64: EFE480AF4C1B73E6 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSPTYDVIVI GLGGMGSAAA HHLSARGARV LGLEKFGPVH NRGSSHGGSR ITRQSYFEDP 

        70         80         90        100        110        120 
AYVPLLLRAY ELYEELERAT GRNVATLCGG VMAGPPDSRT VSGSLRSATE WDLAHEMLDA 

       130        140        150        160        170        180 
KEIRRRFPTL APDDDEVALF EAKAGLLRPE NMVAAHLQLA TRQGAELRFE EPVLRWEPYR 

       190        200        210        220        230        240 
DGVRVHTGEN TYTAGQLVIC PGAWAPQLLA DIGVPITVER QIMYWFQPKG GTGPFVPERH 

       250        260        270        280        290        300 
PVYIWEDADG VQVYGFPAID GPEKGAKVAF FRKGQHTTPE TIDRTVHAHE VRAMADHMSA 

       310        320        330        340        350        360 
LIPDLPGTFL KAATCMYSNT PDEHFVIARH PAHPESVTVA CGFSGHGFKF VPVVGEILAD 

       370        380 
LALTGATAHP IGLFDPARLT APAARGVQP

P40854 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools