ID   MSOX_STRSB              Reviewed;         389 AA.
AC   P40854;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   04-NOV-2008, entry version 56.
DE   RecName: Full=Monomeric sarcosine oxidase;
DE            Short=MSOX;
DE            EC=1.5.3.1;
GN   Name=soxA;
OS   Streptomyces sp. (strain KB210-8SY).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=72592;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-31;
RP   122-143; 230-259 AND 268-283.
RX   MEDLINE=92330009; PubMed=1368326;
RA   Suzuki K., Ogishima M., Sugiyama M., Inouye Y., Nakamura S.,
RA   Imamura S.;
RT   "Molecular cloning and expression of a Streptomyces sarcosine oxidase
RT   gene in Streptomyces lividans.";
RL   Biosci. Biotechnol. Biochem. 56:432-436(1992).
CC   -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Sarcosine + H(2)O + O(2) = glycine +
CC       formaldehyde + H(2)O(2).
CC   -!- COFACTOR: Binds 1 FAD per subunit (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the MSOX/MTOX family. MSOX subfamily.
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DR   EMBL; S39464; AAC60412.2; -; Genomic_DNA.
DR   EMBL; D10623; BAA01473.1; -; Genomic_DNA.
DR   HSSP; P40859; 1EL5.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00516; -; 1.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   Pfam; PF01266; DAO; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; FAD; Flavoprotein;
KW   Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    389       Monomeric sarcosine oxidase.
FT                                /FTId=PRO_0000213764.
FT   NP_BIND       6     36       FAD (Potential).
FT   MOD_RES     315    315       S-8alpha-FAD cysteine (By similarity).
SQ   SEQUENCE   389 AA;  42263 MW;  EFE480AF4C1B73E6 CRC64;
     MSPTYDVIVI GLGGMGSAAA HHLSARGARV LGLEKFGPVH NRGSSHGGSR ITRQSYFEDP
     AYVPLLLRAY ELYEELERAT GRNVATLCGG VMAGPPDSRT VSGSLRSATE WDLAHEMLDA
     KEIRRRFPTL APDDDEVALF EAKAGLLRPE NMVAAHLQLA TRQGAELRFE EPVLRWEPYR
     DGVRVHTGEN TYTAGQLVIC PGAWAPQLLA DIGVPITVER QIMYWFQPKG GTGPFVPERH
     PVYIWEDADG VQVYGFPAID GPEKGAKVAF FRKGQHTTPE TIDRTVHAHE VRAMADHMSA
     LIPDLPGTFL KAATCMYSNT PDEHFVIARH PAHPESVTVA CGFSGHGFKF VPVVGEILAD
     LALTGATAHP IGLFDPARLT APAARGVQP
//