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UniProtKB/Swiss-Prot entry P40859

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MSOX_BACB0
Primary accession number P40859
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1995
Sequence was last modified on February 1, 1996 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 73)
Name and origin of the protein
Protein name Monomeric sarcosine oxidase
Synonyms MSOX
EC 1.5.3.1
Gene name
Name: soxA
Synonyms: sox
From
Bacillus sp. (strain B-0618) [TaxID: 69000] 
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Suzuki K., Sagai H., Imamura S., Sugiyama M.;
"Cloning, sequencing, overexpression in Escherichia coil of a sarcosine oxidase-encoding gene linked to the Bacillus creatinase gene.";
J. Ferment. Bioeng. 77:231-234(1994).
[2]
 SEQUENCE REVISION TO 117.
Suzuki K.;
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases.
[3]
 CHARACTERIZATION, PROTEIN SEQUENCE OF 313-319, AND MASS SPECTROMETRY.
DOI=10.1021/bi982955o; PubMed=10220347 [NCBI, ExPASy, EBI, Israel, Japan]
Wagner M.A., Khanna P., Jorns M.S.;
"Structure of the flavocoenzyme of two homologous amine oxidases: monomeric sarcosine oxidase and N-methyltryptophan oxidase.";
Biochemistry 38:5588-5595(1999).
[4]
 CHARACTERIZATION.
DOI=10.1021/bi000350y; PubMed=10913293 [NCBI, ExPASy, EBI, Israel, Japan]
Wagner M.A., Jorns M.S.;
"Monomeric sarcosine oxidase: 2. Kinetic studies with sarcosine, alternate substrates, and a substrate analogue.";
Biochemistry 39:8825-8829(2000).
[5]
 CHARACTERIZATION.
DOI=10.1021/bi001421w; PubMed=11087383 [NCBI, ExPASy, EBI, Israel, Japan]
Zhao G., Qu J., Davis F.A., Jorns M.S.;
"Inactivation of monomeric sarcosine oxidase by reaction with N-(cyclopropyl)glycine.";
Biochemistry 39:14341-14347(2000).
[6]
 X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
DOI=10.1016/S0969-2126(99)80043-4; PubMed=10368302 [NCBI, ExPASy, EBI, Israel, Japan]
Trickey P., Wagner M.A., Jorns M.S., Mathews F.S.;
"Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme.";
Structure 7:331-345(1999).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D16521; BAA03967.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR I39975; I39975.
3D structure databases
PDB
1EL5; X-ray; 1.80 A; A/B=2-390.[ExPASy / RCSB / EBI]
1EL7; X-ray; 1.90 A; A/B=2-390.[ExPASy / RCSB / EBI]
1EL8; X-ray; 1.90 A; A/B=2-390.[ExPASy / RCSB / EBI]
1EL9; X-ray; 2.00 A; A/B=2-390.[ExPASy / RCSB / EBI]
1ELI; X-ray; 2.00 A; A/B=2-390.[ExPASy / RCSB / EBI]
1L9C; X-ray; 1.90 A; A/B=2-390.[ExPASy / RCSB / EBI]
1L9D; X-ray; 1.95 A; A/B=2-390.[ExPASy / RCSB / EBI]
1L9E; X-ray; 1.85 A; A/B=2-390.[ExPASy / RCSB / EBI]
2A89; X-ray; 1.85 A; A/B=2-390.[ExPASy / RCSB / EBI]
2GB0; X-ray; 1.85 A; A/B=2-390.[ExPASy / RCSB / EBI]
2GF3; X-ray; 1.30 A; A/B=2-390.[ExPASy / RCSB / EBI]
3BHF; X-ray; 2.10 A; A/B=1-390.[ExPASy / RCSB / EBI]
3BHK; X-ray; 1.71 A; A/B=1-390.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1EL5; -.
1EL7; -.
1EL8; -.
1EL9; -.
1ELI; -.
1L9C; -.
1L9D; -.
1L9E; -.
2A89; -.
2GB0; -.
2GF3; -.
3BHF; -.
3BHK; -.
ModBase P40859.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0008115; Molecular function: sarcosine oxidase activity (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00516; -; 1.
PBIL [Tree]
InterPro IPR006076; FAD-dep_OxRdtase.
IPR006281; SoxA_mon.
Graphical view of domain structure.
Pfam PF01266; DAO; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01377; soxA_mon; 1.
BLOCKS P40859.
Other
ProtoNet P40859.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   390  390     Monomeric sarcosine oxidase. PRO_0000213762
NP_BIND   6    36  31     FAD (Potential). 
MOD_RES   316   316        S-8alpha-FAD cysteine. 
STRAND   5    10  6      
HELIX   14    25  12      
STRAND   30    33  4      
STRAND   41    45  5      
STRAND   47    52  6      
HELIX   60    62  3      
HELIX   63    79  17      
STRAND   90    95  6      
HELIX   100   111  12      
STRAND   117   120  4      
HELIX   122   127  6      
STRAND   137   142  6      
STRAND   146   149  4      
HELIX   150   163  14      
STRAND   167   169  3      
STRAND   174   179  6      
STRAND   184   188  5      
STRAND   191   200  10      
HELIX   203   205  3      
HELIX   206   209  4      
HELIX   210   213  4      
STRAND   220   229  10      
HELIX   233   236  4      
HELIX   238   240  3      
STRAND   244   249  6      
STRAND   252   257  6      
STRAND   265   271  7      
TURN   278   280  3      
HELIX   290   302  13      
HELIX   304   306  3      
STRAND   310   320  11      
STRAND   327   331  5      
STRAND   334   341  8      
HELIX   348   350  3      
HELIX   351   364  14      
HELIX   372   374  3      
HELIX   379   381  3      
Sequence information
Length: 390 AA [This is the length of the unprocessed precursor] Molecular weight: 43182 Da [This is the MW of the unprocessed precursor] CRC64: D830491B80230BCF [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSTHFDVIVV GAGSMGMAAG YQLAKQGVKT LLVDAFDPPH TNGSHHGDTR IIRHAYGEGR 

        70         80         90        100        110        120 
EYVPLALRSQ ELWYELEKET HHKIFTKTGV LVFGPKGESA FVAETMEAAK EHSLTVDLLE 

       130        140        150        160        170        180 
GDEINKRWPG ITVPENYNAI FEPNSGVLFS ENCIRAYREL AEARGAKVLT HTRVEDFDIS 

       190        200        210        220        230        240 
PDSVKIETAN GSYTADKLIV SMGAWNSKLL SKLNLDIPLQ PYRQVVGFFE SDESKYSNDI 

       250        260        270        280        290        300 
DFPGFMVEVP NGIYYGFPSF GGCGLKLGYH TFGQKIDPDT INREFGVYPE DESNLRAFLE 

       310        320        330        340        350        360 
EYMPGANGEL KRGAVCMYTK TLDEHFIIDL HPEHSNVVIA AGFSGHGFKF SSGVGEVLSQ 

       370        380        390 
LALTGKTEHD ISIFSINRPA LKESLQKTTI
P40859 in FASTA format

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