ID   MSOX_ARTST              Reviewed;         389 AA.
AC   P40873; O66180;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-NOV-2008, entry version 68.
DE   RecName: Full=Monomeric sarcosine oxidase;
DE            Short=MSOX;
DE            EC=1.5.3.1;
GN   Name=soxA;
OS   Arthrobacter sp. (strain TE1826).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Micrococcineae; Micrococcaceae; Arthrobacter.
OX   NCBI_TaxID=68999;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-6.
RA   Nishiya Y., Imanaka T.;
RT   "Cloning and sequencing of the sarcosine oxidase gene from
RT   Arthrobacter sp. TE1826.";
RL   J. Ferment. Bioeng. 75:239-244(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=98223334; PubMed=9563845; DOI=10.1007/s004380050685;
RA   Nishiya Y., Toda A., Imanaka T.;
RT   "Gene cluster for creatinine degradation in Arthrobacter sp. TE1826.";
RL   Mol. Gen. Genet. 257:581-586(1998).
RN   [3]
RP   MUTAGENESIS OF CYS-265 AND CYS-318.
RX   MEDLINE=95194007; PubMed=7887617;
RA   Nishiya Y., Zuihara S., Imanaka T.;
RT   "Active site analysis and stabilization of sarcosine oxidase by the
RT   substitution of cysteine residues.";
RL   Appl. Environ. Microbiol. 61:367-370(1995).
RN   [4]
RP   MUTAGENESIS OF GLY-13; ALA-14; GLY-15; SER-16; MET-17; GLY-18 AND
RP   ASP-36.
RX   MEDLINE=96271689; PubMed=8779579;
RA   Nishiya Y., Imanaka T.;
RT   "Analysis of interaction between the Arthrobacter sarcosine oxidase
RT   and the coenzyme flavin adenine dinucleotide by site-directed
RT   mutagenesis.";
RL   Appl. Environ. Microbiol. 62:2405-2410(1996).
RN   [5]
RP   MUTAGENESIS OF CYS-318.
RX   MEDLINE=20493514; PubMed=11035956; DOI=10.1006/prep.2000.1299;
RA   Nishiya Y.;
RT   "A mutant sarcosine oxidase in which activity depends on flavin
RT   adenine dinucleotide.";
RL   Protein Expr. Purif. 20:95-97(2000).
CC   -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine.
CC   -!- CATALYTIC ACTIVITY: Sarcosine + H(2)O + O(2) = glycine +
CC       formaldehyde + H(2)O(2).
CC   -!- COFACTOR: Binds 1 FAD per subunit.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Decreases in function by replacement in the G-X-G-
CC       X-X-G motif are suppressed by chloride or bromide ion.
CC   -!- SIMILARITY: Belongs to the MSOX/MTOX family. MSOX subfamily.
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DR   EMBL; D63413; BAA09716.1; -; Genomic_DNA.
DR   EMBL; AB007122; BAA25926.1; -; Genomic_DNA.
DR   PIR; T44248; T44248.
DR   HSSP; P40859; 1L9F.
DR   SMR; P40873; 7-385.
DR   BioCyc; MetaCyc:MON-11002; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR   HAMAP; MF_00516; -; 1.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR006281; SoxA_mon.
DR   Pfam; PF01266; DAO; 1.
DR   TIGRFAMs; TIGR01377; soxA_mon; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; FAD; Flavoprotein;
KW   Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    389       Monomeric sarcosine oxidase.
FT                                /FTId=PRO_0000213760.
FT   NP_BIND       8     38       FAD (Potential).
FT   MOD_RES     318    318       S-8alpha-FAD cysteine (Probable).
FT   MUTAGEN      13     13       G->A: Loss of activity.
FT   MUTAGEN      14     14       A->D,K: Loss of activity.
FT   MUTAGEN      14     14       A->I,V: Decrease in activity.
FT   MUTAGEN      14     14       A->L,W,Y: Weak activity.
FT   MUTAGEN      15     15       G->A: Loss of activity.
FT   MUTAGEN      16     16       S->P: Weak activity. Loss of activity;
FT                                when associated with G-17.
FT   MUTAGEN      17     17       M->G: Weak activity. Loss of activity;
FT                                when associated with P-16.
FT   MUTAGEN      18     18       G->A: Weak activity.
FT   MUTAGEN      36     36       D->A: Loss of activity.
FT   MUTAGEN      36     36       D->E: Almost no change in activity.
FT   MUTAGEN      36     36       D->N: 100-fold decrease in activity.
FT   MUTAGEN     265    265       C->A,D: Decrease in activity. Stabilizes
FT                                soxA against chemicals and metal ions.
FT   MUTAGEN     265    265       C->R,S: Almost no change in activity.
FT                                Stabilizes soxA against chemicals and
FT                                metal ions.
FT   MUTAGEN     318    318       C->S: Weak activity.
FT   CONFLICT     39     39       H -> D (in Ref. 2; BAA09716/BAA25926).
FT   CONFLICT    316    316       D -> A (in Ref. 2; BAA09716/BAA25926).
SQ   SEQUENCE   389 AA;  43294 MW;  77437C4F12B4AC7F CRC64;
     MSIKKDYDVI VVGAGSMGMA AGYYLSKQGV KTLLVDSFHP PHTNGSHHGD TRIIRHAYGE
     GREYVPFALR AQELWYELEK ETHHKIFTKT GVLVFGPKGE APFVAETMEA AKEHSLDVDL
     LEGSEINKRW PGVTVPENYN AIFEKNSGVL FSENCIRAYR ELAEANGAKV LTYTPVEDFE
     IAEDFVKIQT AYGSFTASKL IVSMGAWNSK LLSKLNIEIP LQPYRQVVGF FECDEKKYSN
     THGYPAFMVE VPTGIYYGFP SFGGCGLKIG YHTYGQKIDP DTINREFGIY PEDEGNIRKF
     LETYMPGATG ELKSGDVCMY TKTPDEHFVI DLHPQFSNVA IAAGFSGHGF KFSSVVGETL
     SQLAVTGKTE HDISIFSINR PALKQKETI
//