[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1074/jbc.270.31.18252; PubMed=7543100 [NCBI, ExPASy, EBI, Israel, Japan] Chlumsky L.J., Zhang L., Jorns M.S.; "Sequence analysis of sarcosine oxidase and nearby genes reveals homologies with key enzymes of folate one-carbon metabolism."; J. Biol. Chem. 270:18252-18259(1995).
[2]	PROTEIN SEQUENCE OF 161-187 AND 334-404. STRAIN=U-96; PubMed=1939012 [NCBI, ExPASy, EBI, Israel, Japan] Suzuki H., Kawamura-Konishi Y.; "Cysteine residues in the active site of Corynebacterium sarcosine oxidase."; J. Biochem. 109:909-917(1991).
[3]	PARTIAL PROTEIN SEQUENCE. STRAIN=U-96; PubMed=3202887 [NCBI, ExPASy, EBI, Israel, Japan] Suzuki H., Kawamura-Konishi Y.; "Presence of AMP binding sequence in subunit B of Corynebacterium sarcosine oxidase."; Biochem. Int. 17:577-583(1988).
[4]	CHARACTERIZATION. DOI=10.1021/bi00092a024; PubMed=7692961 [NCBI, ExPASy, EBI, Israel, Japan] Chlumsky L.J., Zhang L., Ramsey A.J., Jorns M.S.; "Preparation and properties of recombinant corynebacterial sarcosine oxidase: evidence for posttranslational modification during turnover with sarcosine."; Biochemistry 32:11132-11142(1993).
[5]	FMN-BINDING. DOI=10.1021/bi952995h; PubMed=8611516 [NCBI, ExPASy, EBI, Israel, Japan] Willie A., Edmondson D.E., Jorns M.S.; "Sarcosine oxidase contains a novel covalently bound FMN."; Biochemistry 35:5292-5299(1996).
[6]	PROTEIN SEQUENCE OF 174-178, FMN-BINDING AT HIS-173, AND MASS SPECTROMETRY. DOI=10.1021/bi972705s; PubMed=9485355 [NCBI, ExPASy, EBI, Israel, Japan] Chlumsky L.J., Sturgess A.W., Nieves E., Jorns M.S.; "Identification of the covalent flavin attachment site in sarcosine oxidase."; Biochemistry 37:2089-2095(1998).
[7]	PROTEIN SEQUENCE OF 174-178, FMN-BINDING AT HIS-173, AND MASS SPECTROMETRY. DOI=10.1023/A:1014135216860; PubMed=11902668 [NCBI, ExPASy, EBI, Israel, Japan] Mukouyama E.B., Ohsawa H., Suzuki H.; "Cofactors in sarcosine oxidase from Corynebacterium sp. U-96."; J. Protein Chem. 21:59-64(2002).

Comments

FUNCTION: Catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide and 5,10-methylenetetrahydrofolate.
CATALYTIC ACTIVITY: Sarcosine + H₂O + O₂ = glycine + formaldehyde + H₂O₂.
COFACTOR: Binds 1 FAD per subunit.
COFACTOR: Binds 1 FMN covalently.
SUBUNIT: Heterotetramer (alpha 100 kDa, beta 42 kDa, gamma 20 kDa, and delta 6 kDa).
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the soxB family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U23955; AAC43459.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

PX0049; PX0049.

3D structure databases

SMR

P40875; 3-405.

ModBase

P40875.

Family and domain databases

InterPro

IPR006076; FAD-dep_OxRdtase.
IPR006278; SoxB.
Graphical view of domain structure.

Pfam

PF01266; DAO; 1.
Pfam graphical view of domain structure.

TIGR01373; soxB; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; FMN; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 405`	`405`	`Sarcosine oxidase subunit beta.`	`PRO_0000072044`
`MOD_RES`	`173 173`		`Tele-8alpha-FMN histidine.`
`VARIANT`	`167 167`	`1`	`R -> Y (in strain: U-96).`
`VARIANT`	`172 173`	`2`	`KH -> CK (in strain: U-96).`
`VARIANT`	`178 178`	`1`	`W -> T (in strain: U-96).`
`VARIANT`	`182 182`	`1`	`R -> N (in strain: U-96).`
`VARIANT`	`359 359`	`1`	`K -> FC (in strain: U-96).`
`VARIANT`	`366 366`	`1`	`F -> Y (in strain: U-96).`
`VARIANT`	`370 370`	`1`	`H -> E (in strain: U-96).`
`VARIANT`	`374 374`	`1`	`N -> A (in strain: U-96).`
`VARIANT`	`377 379`	`3`	`AHA -> PKK (in strain: U-96).`
`VARIANT`	`385 385`	`1`	`S -> A (in strain: U-96).`

Sequence information

Length: 405 AA [This is the length of the unprocessed precursor]

Molecular weight: 43987 Da [This is the MW of the unprocessed precursor]

CRC64: 401141810BA60CDE [This is a checksum on the sequence]

        10         20         30         40         50         60 
MADLLPEHPE FLWANPEPKK SYDVVIVGGG GHGLATAYYL AKNHGITNVA VLEKGWLAGG 

        70         80         90        100        110        120 
NMARNTTIIR SNYLWDESAG IYEKSLKLWE QLPEELDYDF LFSQRGVLNL AHTLGDVRES 

       130        140        150        160        170        180 
VRRVEANKFN GVDAEWLTPE QVKEVCPIIN IGDDIRYPVM GATYQPRAGI AKHDHVAWAF 

       190        200        210        220        230        240 
ARKANEMGVD IIQNCEVTGF LKDGEKVTGV KTTRGTIHAG KVALAGAGHS SVLAELAGFE 

       250        260        270        280        290        300 
LPIQSHPLQA LVSELFEPVH PTVVMSNHIH VYVSQAHKGE LVMGAGIDSY NGYGQRGAFH 

       310        320        330        340        350        360 
VIEEQMAAAV ELFPIFARAH VLRTWGGIVD TTMDASPIIS KTPIQNLYVN CGWGTGGFKG 

       370        380        390        400 
TPGAGFTLAH TIANDEAHAL NAPFSLERFE TGHLIDEHGA AAVAH

P40875 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools