ID   SOXB_CORS1              Reviewed;         405 AA.
AC   P40875; Q46335;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   25-NOV-2008, entry version 65.
DE   RecName: Full=Sarcosine oxidase subunit beta;
DE            Short=Sarcosine oxidase subunit B;
DE            EC=1.5.3.1;
GN   Name=soxB;
OS   Corynebacterium sp. (strain P-1).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Corynebacteriaceae; Corynebacterium.
OX   NCBI_TaxID=69006;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=95355441; PubMed=7543100; DOI=10.1074/jbc.270.31.18252;
RA   Chlumsky L.J., Zhang L., Jorns M.S.;
RT   "Sequence analysis of sarcosine oxidase and nearby genes reveals
RT   homologies with key enzymes of folate one-carbon metabolism.";
RL   J. Biol. Chem. 270:18252-18259(1995).
RN   [2]
RP   PROTEIN SEQUENCE OF 161-187 AND 334-404.
RC   STRAIN=U-96;
RX   MEDLINE=92041745; PubMed=1939012;
RA   Suzuki H., Kawamura-Konishi Y.;
RT   "Cysteine residues in the active site of Corynebacterium sarcosine
RT   oxidase.";
RL   J. Biochem. 109:909-917(1991).
RN   [3]
RP   PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=U-96;
RX   MEDLINE=89076343; PubMed=3202887;
RA   Suzuki H., Kawamura-Konishi Y.;
RT   "Presence of AMP binding sequence in subunit B of Corynebacterium
RT   sarcosine oxidase.";
RL   Biochem. Int. 17:577-583(1988).
RN   [4]
RP   CHARACTERIZATION.
RX   MEDLINE=94032225; PubMed=7692961; DOI=10.1021/bi00092a024;
RA   Chlumsky L.J., Zhang L., Ramsey A.J., Jorns M.S.;
RT   "Preparation and properties of recombinant corynebacterial sarcosine
RT   oxidase: evidence for posttranslational modification during turnover
RT   with sarcosine.";
RL   Biochemistry 32:11132-11142(1993).
RN   [5]
RP   FMN-BINDING.
RX   PubMed=8611516; DOI=10.1021/bi952995h;
RA   Willie A., Edmondson D.E., Jorns M.S.;
RT   "Sarcosine oxidase contains a novel covalently bound FMN.";
RL   Biochemistry 35:5292-5299(1996).
RN   [6]
RP   PROTEIN SEQUENCE OF 174-178, FMN-BINDING AT HIS-173, AND MASS
RP   SPECTROMETRY.
RX   PubMed=9485355; DOI=10.1021/bi972705s;
RA   Chlumsky L.J., Sturgess A.W., Nieves E., Jorns M.S.;
RT   "Identification of the covalent flavin attachment site in sarcosine
RT   oxidase.";
RL   Biochemistry 37:2089-2095(1998).
RN   [7]
RP   PROTEIN SEQUENCE OF 174-178, FMN-BINDING AT HIS-173, AND MASS
RP   SPECTROMETRY.
RX   PubMed=11902668; DOI=10.1023/A:1014135216860;
RA   Mukouyama E.B., Ohsawa H., Suzuki H.;
RT   "Cofactors in sarcosine oxidase from Corynebacterium sp. U-96.";
RL   J. Protein Chem. 21:59-64(2002).
CC   -!- FUNCTION: Catalyzes the oxidative demethylation of sarcosine to
CC       yield glycine, hydrogen peroxide and 5,10-
CC       methylenetetrahydrofolate.
CC   -!- CATALYTIC ACTIVITY: Sarcosine + H(2)O + O(2) = glycine +
CC       formaldehyde + H(2)O(2).
CC   -!- COFACTOR: Binds 1 FAD per subunit.
CC   -!- COFACTOR: Binds 1 FMN covalently.
CC   -!- SUBUNIT: Heterotetramer (alpha 100 kDa, beta 42 kDa, gamma 20 kDa,
CC       and delta 6 kDa).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the soxB family.
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DR   EMBL; U23955; AAC43459.1; -; Genomic_DNA.
DR   PIR; PX0049; PX0049.
DR   SMR; P40875; 3-405.
DR   BioCyc; MetaCyc:MON-8522; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0008115; F:sarcosine oxidase activity; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0046653; P:tetrahydrofolate metabolic process; IEA:InterPro.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR006278; SoxB.
DR   Pfam; PF01266; DAO; 1.
DR   TIGRFAMs; TIGR01373; soxB; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; FMN;
KW   Oxidoreductase.
FT   CHAIN         1    405       Sarcosine oxidase subunit beta.
FT                                /FTId=PRO_0000072044.
FT   MOD_RES     173    173       Tele-8alpha-FMN histidine.
FT   VARIANT     167    167       R -> Y (in strain: U-96).
FT   VARIANT     172    173       KH -> CK (in strain: U-96).
FT   VARIANT     178    178       W -> T (in strain: U-96).
FT   VARIANT     182    182       R -> N (in strain: U-96).
FT   VARIANT     359    359       K -> FC (in strain: U-96).
FT   VARIANT     366    366       F -> Y (in strain: U-96).
FT   VARIANT     370    370       H -> E (in strain: U-96).
FT   VARIANT     374    374       N -> A (in strain: U-96).
FT   VARIANT     377    379       AHA -> PKK (in strain: U-96).
FT   VARIANT     385    385       S -> A (in strain: U-96).
SQ   SEQUENCE   405 AA;  43987 MW;  401141810BA60CDE CRC64;
     MADLLPEHPE FLWANPEPKK SYDVVIVGGG GHGLATAYYL AKNHGITNVA VLEKGWLAGG
     NMARNTTIIR SNYLWDESAG IYEKSLKLWE QLPEELDYDF LFSQRGVLNL AHTLGDVRES
     VRRVEANKFN GVDAEWLTPE QVKEVCPIIN IGDDIRYPVM GATYQPRAGI AKHDHVAWAF
     ARKANEMGVD IIQNCEVTGF LKDGEKVTGV KTTRGTIHAG KVALAGAGHS SVLAELAGFE
     LPIQSHPLQA LVSELFEPVH PTVVMSNHIH VYVSQAHKGE LVMGAGIDSY NGYGQRGAFH
     VIEEQMAAAV ELFPIFARAH VLRTWGGIVD TTMDASPIIS KTPIQNLYVN CGWGTGGFKG
     TPGAGFTLAH TIANDEAHAL NAPFSLERFE TGHLIDEHGA AAVAH
//