ID   KYE1_KLULA              Reviewed;         398 AA.
AC   P40952; Q6CXE2;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2004, sequence version 2.
DT   25-NOV-2008, entry version 52.
DE   RecName: Full=NADPH dehydrogenase 1;
DE            EC=1.6.99.1;
DE   AltName: Full=Old yellow enzyme 1;
GN   Name=KYE1; OrderedLocusNames=KLLA0A09075g;
OS   Kluyveromyces lactis (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=28985;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37;
RX   MEDLINE=95321018; PubMed=7597850;
RA   Miranda M., Ramirez J., Guevara S., Ongay-Larios L., Pena A.,
RA   Coria R.;
RT   "Nucleotide sequence and chromosomal localization of the gene encoding
RT   the Old Yellow Enzyme from Kluyveromyces lactis.";
RL   Yeast 11:459-465(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S.,
RA   Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E.,
RA   Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V.,
RA   Barnay S., Blanchin S., Beckerich J.-M., Beyne E., Bleykasten C.,
RA   Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A.,
RA   Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A.,
RA   Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R.,
RA   Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H.,
RA   Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O.,
RA   Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A.,
RA   Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B.,
RA   Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A.,
RA   Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J.,
RA   Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- CATALYTIC ACTIVITY: NADPH + acceptor = NADP(+) + reduced acceptor.
CC   -!- COFACTOR: FMN.
CC   -!- SUBUNIT: Homodimer or heterodimer.
CC   -!- SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase
CC       family.
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DR   EMBL; L37452; AAA98815.1; -; Genomic_DNA.
DR   EMBL; CR382121; CAH02985.1; -; Genomic_DNA.
DR   PIR; S55844; S55844.
DR   RefSeq; XP_451397.1; -.
DR   HSSP; Q02899; 1OYC.
DR   SMR; P40952; 5-398.
DR   GeneID; 2896828; -.
DR   KEGG; kla:KLLA0A09075g; -.
DR   HOGENOM; P40952; -.
DR   GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR   GO; GO:0003959; F:NADPH dehydrogenase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR001155; OxRdtase_FMN_N.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Pfam; PF00724; Oxidored_FMN; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Flavoprotein; FMN; NADP; Oxidoreductase.
FT   CHAIN         1    398       NADPH dehydrogenase 1.
FT                                /FTId=PRO_0000194476.
FT   ACT_SITE    196    196       Proton donor (By similarity).
FT   BINDING      37     37       FMN (By similarity).
FT   BINDING     191    191       FMN (By similarity).
FT   BINDING     191    191       Substrate (By similarity).
FT   BINDING     194    194       Substrate (By similarity).
FT   BINDING     243    243       FMN (By similarity).
FT   BINDING     348    348       FMN (By similarity).
FT   BINDING     375    375       Substrate (By similarity).
FT   CONFLICT    256    256       G -> V (in Ref. 1; AAA98815).
SQ   SEQUENCE   398 AA;  44780 MW;  AA40CCC0C2BE6EC1 CRC64;
     MSFMNFEPKP LADTDIFKPI KIGNTELKHR VVMPALTRMR ALHPGNVPNP DWAVEYYRQR
     SQYPGTMIIT EGAFPSAQSG GYDNAPGVWS EEQLAQWRKI FKAIHDNKSF VWVQLWVLGR
     QAFADNLARD GLRYDSASDE VYMGEDEKER AIRSNNPQHG ITKDEIKQYI RDYVDAAKKC
     IDAGADGVEI HSANGYLLNQ FLDPISNKRT DEYGGSIENR ARFVLEVVDA VVDAVGAERT
     SIRFSPYGVF GTMSGGSDPV LVAQFAYVLA ELEKRAKAGK RLAYVDLVEP RVTSPFQPEF
     EGWYKGGTNE FVYSVWKGNV LRVGNYALDP DAAITDSKNP NTLIGYGRAF IANPDLVERL
     EKGLPLNQYD RPSFYKMSAE GYIDYPTYEE AVAKGYKK
//