ID   PUO_MICRU               Reviewed;         478 AA.
AC   P40974;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-NOV-2008, entry version 39.
DE   RecName: Full=Putrescine oxidase;
DE            EC=1.4.3.10;
GN   Name=puo;
OS   Micrococcus rubens (Deinococcus erythromyxa) (Kocuria rosea).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Micrococcineae; Micrococcaceae; Kocuria.
OX   NCBI_TaxID=1275;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=ATCC 186 / DSM 20447 / IFO 3768 / JCM 11614 / NCTC 7523;
RX   MEDLINE=93232762; PubMed=8473854;
RA   Ishizuka H., Horinouchi S., Beppu T.;
RT   "Putrescine oxidase of Micrococcus rubens: primary structure and
RT   Escherichia coli.";
RL   J. Gen. Microbiol. 139:425-432(1993).
CC   -!- CATALYTIC ACTIVITY: Putrescine + O(2) + H(2)O = 4-aminobutanal +
CC       NH(3) + H(2)O(2).
CC   -!- COFACTOR: FAD.
CC   -!- SIMILARITY: Belongs to the flavin monoamine oxidase family.
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DR   EMBL; D12511; BAA02074.1; -; Genomic_DNA.
DR   PIR; A47693; A47693.
DR   HSSP; P27338; 1GOS.
DR   BioCyc; MetaCyc:MON-23; -.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0050232; F:putrescine oxidase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000759; Adrndx_reductase.
DR   InterPro; IPR001613; Amineoxid_fl.
DR   InterPro; IPR002937; Amino_oxidase.
DR   Pfam; PF01593; Amino_oxidase; 1.
DR   PRINTS; PR00419; ADXRDTASE.
DR   PRINTS; PR00757; AMINEOXDASEF.
DR   ProDom; PD000139; FAD_pyr_redox; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; FAD; Flavoprotein; Oxidoreductase.
FT   CHAIN         1    478       Putrescine oxidase.
FT                                /FTId=PRO_0000099866.
FT   NP_BIND      15     70       FAD (Potential).
SQ   SEQUENCE   478 AA;  51914 MW;  9C4567051B010582 CRC64;
     MTDQRTLGSE TAIERDVVVV GAGPAGLMAA RTLVAAGRTV AVLEARDRVG GRTWSKTVDG
     AFLEIGGQWI SPDQTELLAL VDELGLETYQ RYREGESVYL APDGTRHTYT GSMFPAGEST
     IVEMEKLVAL LDGLVAEIGA TEPWAHPAAR ELDTISFHHW LRQHSDDEAA CSNIGIFVAG
     GMLTKPAHAF SVLQAVLMAA SAGSFSNLVD EDFILDRRVV GGMQSVSETM AAELGEDVVF
     LDTPVRTIRW AGDGGTYAEH VPGTPVTVWS DRLTVRAKDV VVAVPPNLYS RISFEPPLPR
     LQHQMHQHQS LGLVIKVHAV YETPFWRDKG LSGTGFGAHE LSQEVYDNTN HGDPRGTLVG
     FVSDERADEL FGLPAEERRR LILESLSHYL GEEALHPVVY YESDFGSEEW TRGAYAASYD
     LGGLHRYGAH QRTPVGPIRW ACSDLAAEGY QHVDGALRQG RLAAAEVLGA GSLTGAER
//