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UniProtKB/Swiss-Prot entry P41019

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU3_BACME
Primary accession number P41019
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1995
Sequence was last modified on February 1, 1995 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 54)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase
Synonyms EC 1.1.1.85
Beta-IPM dehydrogenase
IMDH
3-IPM-DH
Gene name
Name: leuB
Synonyms: leuC
From
Bacillus megaterium [TaxID: 1404] 
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=DSM 319;
DOI=10.1007/s002530050155; PubMed=7764969 [NCBI, ExPASy, EBI, Israel, Japan]
Meinhardt F., Busskamp M., Wittchen K.D.;
"Cloning and sequencing of the leu C and npr M genes and a putative spo IV gene from Bacillus megaterium DSM319.";
Appl. Microbiol. Biotechnol. 41:344-351(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X65184; CAA46295.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR I40226; I40226.
3D structure databases
HSSP P12010; 2AYQ. [HSSP ENTRY / PDB]
ModBase P41019.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030145; Molecular function: manganese ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0009098; Biological process: leucine biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
HAMAP MF_01033; -; 1.
PBIL [Tree]
InterPro IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00169; leuB; 1.
PROSITE PS00470; IDH_IMDH; 1.
ProtoNet P41019.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   367  367     3-isopropylmalate dehydrogenase. PRO_0000083641
NP_BIND   74    87  14     NAD (By similarity). 
NP_BIND   278   290  13     NAD (By similarity). 
METAL   220   220        Magnesium or manganese (By similarity). 
METAL   244   244        Magnesium or manganese (By similarity). 
METAL   248   248        Magnesium or manganese (By similarity). 
BINDING   94    94        Substrate (By similarity). 
BINDING   104   104        Substrate (By similarity). 
BINDING   132   132        Substrate (By similarity). 
BINDING   220   220        Substrate (By similarity). 
SITE   139   139  1     Important for catalysis (By similarity). 
SITE   188   188  1     Important for catalysis (By similarity). 
Sequence information
Length: 367 AA [This is the length of the unprocessed precursor] Molecular weight: 39942 Da [This is the MW of the unprocessed precursor] CRC64: DC04D48E0EEAB0DD [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MTKKVAVLPG DGGQSNQRRC CRLKAIGERL RHQFEFTYGL IGGAAIDEAG SSSSRSTIET 

        70         80         90        100        110        120 
CNEADAVLLG SVGGPKWDRF PSHLRPEKGL LAIRKELDLY ANLRPVTFYD SLADASPLKK 

       130        140        150        160        170        180 
EYIEGVDFII VRELTGGLYF GKPSDRRTEG NKETVVDTLF YKRTEIERII ASIDTAVNRR 

       190        200        210        220        230        240 
KKVTSVDKAN VLESSRVWRE VAEEVAKDYP DVELEHMLVD SAAMQLIRNP KYFDVVVTEN 

       250        260        270        280        290        300 
MFGDILSDEA SMLTGSLGML PSASLTADGP SLYEPVHGSA PDIAGQNKAN PIAAAILSAA 

       310        320        330        340        350        360 
MLLRHSFGLE KEAAVIEQAV ESVLHAGHRT ADLADGNHYL GTDKMVEVIT AVIANDSAIS 


SIMTASR
P41019 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
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