[1]	NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE. TISSUE=Heart; DOI=10.1016/0014-5793(92)80248-F; PubMed=1577158 [NCBI, ExPASy, EBI, Israel, Japan] Arizmendi J.M., Runswick M.J., Skehel J.M., Walker J.E.; "NADH: ubiquinone oxidoreductase from bovine heart mitochondria. A fourth nuclear encoded subunit with a homologue encoded in chloroplast genomes."; FEBS Lett. 301:237-242(1992).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Crossbred X Angus; TISSUE=Liver; NIH - Mammalian Gene Collection (MGC) project; Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster (Potential).
SUBUNIT: Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme.
SUBCELLULAR LOCATION: Mitochondrion.
SIMILARITY: Belongs to the complex I 20 kDa subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X65020; CAA46154.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC109716; AAI09717.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

S22371; S22371.

NP_001033111.1; -.

3D structure databases

ModBase

P42026.

Family and domain databases

InterPro

IPR006138; NADH_DHase_20kDa_su.
IPR014406; NiFe_hyd_3_ssu/Q_oxred_NuoB.
IPR006137; OxRdtase_q6.
Graphical view of domain structure.

PANTHER

PTHR11995:SF2; NADH_DH_20kDa; 1.
PTHR11995; NiFe_hyd_3_ssu/Q_oxred_NuoB; 1.

Pfam

PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01957; nuoB_fam; 1.

PROSITE

PS01150; COMPLEX1_20K; 1.

BLOCKS

P42026.

Genome annotation databases

Ensembl

ENSBTAG00000019419; Bos taurus. [Contig view]

GeneID

338079; -.

KEGG

bta:338079; -.

Phylogenomic databases

HOVERGEN

P42026; -.

Other

ProtoNet

P42026.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Respiratory chain; Transit peptide; Transport; Ubiquinone.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 37`	`37`	`Mitochondrion.`
`CHAIN`	`38 216`	`179`	`NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial.`	`PRO_0000020026`
`METAL`	`91 91`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`92 92`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`156 156`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`186 186`		`Iron-sulfur (4Fe-4S) (Potential).`

Sequence information

Length: 216 AA [This is the length of the unprocessed precursor]

Molecular weight: 23771 Da [This is the MW of the unprocessed precursor]

CRC64: 514B8A63C59BE641 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAALAALRLL HPILAVRSGV GAALQVRGVH SSMAADSPSS TQPAVSQARA VVPKPAALPS 

        70         80         90        100        110        120 
SRGEYVVAKL DDLINWARRS SLWPMTFGLA CCAVEMMHMA APRYDMDRFG VVFRASPRQS 

       130        140        150        160        170        180 
DVMIVAGTLT NKMAPALRKV YDQMPEPRYV VSMGSCANGG GYYHYSYSVV RGCDRIVPVD 

       190        200        210 
IYVPGCPPTA EALLYGILQL QKKIKREKRL RIWYRR

P42026 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools