NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Shogun;
TISSUE=Floret;
DOI=10.1104/pp.108.2.859; PubMed=7610188 [NCBI, ExPASy, EBI, Israel, Japan]
Pogson B.J., Downs C.G., Davies K.M., Morris S.C., Buchanan-Wollaston V.;
"Nucleotide sequence of a cDNA clone from broccoli with high identity with the PSST subunit of NADH:ubiquinone oxidoreductase.";
Plant Physiol. 108:859-860(1995).

Comments

FUNCTION: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
CATALYTIC ACTIVITY: NADH + ubiquinone = NAD⁺ + ubiquinol.
CATALYTIC ACTIVITY: NADH + acceptor = NAD⁺ + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster (Potential).
SUBUNIT: Complex I is composed of 45 different subunits This is a component of the iron-sulfur (IP) fragment of the enzyme (By similarity).
SUBCELLULAR LOCATION: Mitochondrion.
SIMILARITY: Belongs to the complex I 20 kDa subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X82274; CAA57725.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S48826; S48826.

3D structure databases

ModBase

P42027.

Ontologies

GO:0005746;	Cellular component: mitochondrial respiratory chain (inferred from electronic annotation from UniProtKB-KW).
GO:0051539;	Molecular function: 4 iron, 4 sulfur cluster binding (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0008137;	Molecular function: NADH dehydrogenase (ubiquinone) activity (inferred from electronic annotation from InterPro).
GO:0006120;	Biological process: mitochondrial electron transport, NADH to ubiquinone (inferred from electronic annotation from InterPro).
GO:0006810;	Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR006138; NADH_DHase_20kDa_su.
IPR014406; NiFe_hyd_3_ssu/Q_oxred_NuoB.
IPR006137; OxRdtase_q6.
Graphical view of domain structure.

PANTHER

PTHR11995:SF2; NADH_DH_20kDa; 1.
PTHR11995; NiFe_hyd_3_ssu/Q_oxred_NuoB; 1.

Pfam

PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01957; nuoB_fam; 1.

PROSITE

PS01150; COMPLEX1_20K; 1.

ProtoNet

P42027.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Electron transport; Iron; Iron-sulfur; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Respiratory chain; Transit peptide; Transport; Ubiquinone.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 ?`		`Mitochondrion.`
`CHAIN`	`? 215`		`NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial.`	`PRO_0000020034`
`METAL`	`90 90`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`91 91`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`155 155`		`Iron-sulfur (4Fe-4S) (Potential).`
`METAL`	`185 185`		`Iron-sulfur (4Fe-4S) (Potential).`

Sequence information

Length: 215 AA [This is the length of the unprocessed precursor]

Molecular weight: 23839 Da [This is the MW of the unprocessed precursor]

CRC64: E39E62D038C11FC3 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAMITRNTAT RLPLVLQSHR AAAVSHLHTS LPALSPATTP TSYTRPGPPS TSAPPPGLSK 

        70         80         90        100        110        120 
TAEFVISKVD DLMNWARRGS IWPMTFGLAC CAVEMMHTGA ARYDLDRFGI IFRPSPRQSD 

       130        140        150        160        170        180 
CMIVAGTLTN KMAPALRKVY DQMPEPRWVI SMGSCANGGG YYHYSYSVVR GCDRIVPVDI 

       190        200        210 
YVPGCPPTAE ALLYGLLQLQ KKINRRKDFL HWWNK

P42027 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools