ID   PCPB_SPHCR              Reviewed;         538 AA.
AC   P42535; Q8KMS8;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 4.
DT   25-NOV-2008, entry version 55.
DE   RecName: Full=Pentachlorophenol 4-monooxygenase;
DE            EC=1.14.13.50;
DE   AltName: Full=Pentachlorophenol hydroxylase;
GN   Name=pcpB;
OS   Sphingobium chlorophenolicum.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=46429;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-14.
RC   STRAIN=ATCC 39723 / DSM 6824 / L-1;
RX   MEDLINE=93123159; PubMed=7678243;
RA   Orser C.S., Lange C.C., Xun L., Zahrt T.C., Schneider B.J.;
RT   "Cloning, sequence analysis, and expression of the Flavobacterium
RT   pentachlorophenol-4-monooxygenase gene in Escherichia coli.";
RL   J. Bacteriol. 175:411-416(1993).
RN   [2]
RP   SEQUENCE REVISION TO 536, FUNCTION, AND INDUCTION.
RC   STRAIN=ATCC 39723 / DSM 6824 / L-1;
RX   MEDLINE=22159920; PubMed=12169590;
RX   DOI=10.1128/JB.184.17.4672-4680.2002;
RA   Cai M., Xun L.;
RT   "Organization and regulation of pentachlorophenol-degrading genes in
RT   Sphingobium chlorophenolicum ATCC 39723.";
RL   J. Bacteriol. 184:4672-4680(2002).
CC   -!- FUNCTION: Dechlorination of pentachlorophenol to
CC       tetrachlorohydroquinone. Removes also hydrogen and nitro, amino,
CC       and cyano groups from benzene ring at the para position in
CC       relation to the hydroxyl of phenol.
CC   -!- CATALYTIC ACTIVITY: Pentachlorophenol + 2 NADPH + O(2) = 2,3,5,6-
CC       tetrachlorohydroquinone + 2 NADP(+) + chloride + H(2)O.
CC   -!- CATALYTIC ACTIVITY: 2,3,5,6-tetrachlorophenol + NADPH + O(2) =
CC       2,3,5,6-tetrachlorohydroquinone + NADP(+) + H(2)O.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- PATHWAY: Xenobiotic degradation; pentachlorophenol degradation.
CC   -!- INDUCTION: By polychlorinated phenols.
CC   -!- SIMILARITY: Belongs to the pheA/tfdB FAD monooxygenase family.
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DR   EMBL; U12290; AAF15368.2; -; Genomic_DNA.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0018677; F:pentachlorophenol monooxygenase activity; IEA:EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000759; Adrndx_reductase.
DR   InterPro; IPR002938; mOase_FAD_bd.
DR   InterPro; IPR003042; Rng_hydrolase.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   PRINTS; PR00419; ADXRDTASE.
DR   PRINTS; PR00420; RNGMNOXGNASE.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Direct protein sequencing; FAD;
KW   Flavoprotein; Monooxygenase; NADP; Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    538       Pentachlorophenol 4-monooxygenase.
FT                                /FTId=PRO_0000214044.
FT   NP_BIND      16     45       FAD (Potential).
FT   NP_BIND     288    298       FAD (Potential).
SQ   SEQUENCE   538 AA;  59992 MW;  CB545A9A1DCE8AB9 CRC64;
     MSTYPINAPG QSADAAVLIV GGGPTGLIAA NELLRRGVSC RMIDRLPVAH QTSKSCTIHA
     RSMEMMEHIG IAARYIETGV RSNGFTFNFE NTDANALLDF SVLPGRYPFI TIYNQNETER
     VLRHDLEATY SFQPEWGTQL LALNQDENGI RADLRLKDGT KQTISPRWVI GADGVRSRVR
     ECLGIAYEGE DYEENVLQMM DVGIQDFEAG DDWIHYFIGQ DKFVFVTKLP GSNYRVIISD
     LGGANKSNLE ETREAFQGYL SSFDDHATLD EPRWATKWRV WKRMATAYRK GNVFLAGDAA
     HCHSPSGGSG MNVGMQDAFN LGWKIAMVER GEAKPDLLDT YHTERTPVAQ QLLEGTHAMH
     EIIMGHGKGL TDRIELTQAP GWHDAATYRV SGMSYNYRDQ LVSFNDDRLA GPSAGDRIPD
     AELAPRIRLF DLVRNTRPTL LVAPATEAEV AEAEKLRDLI REQWPLVKPV LVRPQGSEES
     IEGDVHVDSY GQLKREWGDN AKGWAALLRP DNYIHARAGL DRGDLLVQAI DAMLVRCA
//