ID   PNTB_HAEIN              Reviewed;         474 AA.
AC   P43010;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-NOV-2008, entry version 54.
DE   RecName: Full=NAD(P) transhydrogenase subunit beta;
DE            EC=1.6.1.2;
DE   AltName: Full=Nicotinamide nucleotide transhydrogenase subunit beta;
DE   AltName: Full=Pyridine nucleotide transhydrogenase subunit beta;
GN   Name=pntB; OrderedLocusNames=HI1363;
OS   Haemophilus influenzae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=727;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   MEDLINE=95350630; PubMed=7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M.,
RA   Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D.,
RA   Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C.,
RA   Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M.,
RA   Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O.,
RA   Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 244-474.
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   MEDLINE=96186898; PubMed=8635745; DOI=10.1016/0378-1119(95)00777-6;
RA   Chandler M.S., Smith R.A.;
RT   "Characterization of the Haemophilus influenzae topA locus: DNA
RT   topoisomerase I is required for genetic competence.";
RL   Gene 169:25-31(1996).
CC   -!- FUNCTION: The transhydrogenation between NADH and NADP is coupled
CC       to respiration and ATP hydrolysis and functions as a proton pump
CC       across the membrane (By similarity).
CC   -!- CATALYTIC ACTIVITY: NADPH + NAD(+) = NADP(+) + NADH.
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane
CC       protein (By similarity).
CC   -!- SIMILARITY: Belongs to the PNT beta subunit family.
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DR   EMBL; L42023; AAC23010.1; -; Genomic_DNA.
DR   EMBL; U20964; AAC43725.1; -; Genomic_DNA.
DR   PIR; F64119; F64119.
DR   RefSeq; NP_439514.1; -.
DR   HSSP; P11024; 1D4O.
DR   SMR; P43010; 308-474.
DR   GeneID; 950280; -.
DR   GenomeReviews; L42023_GR; HI1363.
DR   KEGG; hin:HI1363; -.
DR   NMPDR; fig|71421.1.peg.1299; -.
DR   TIGR; HI1363; -.
DR   HOGENOM; P43010; -.
DR   BioCyc; HINF71421:HI_1363-MON; -.
DR   GO; GO:0016021; C:integral to membrane; IEA:InterPro.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008750; F:NAD(P)+ transhydrogenase (AB-specific) acti...; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR012136; NADH_DH_b.
DR   InterPro; IPR004003; NADP_transhyd_b.
DR   Pfam; PF02233; PNTB; 1.
DR   PIRSF; PIRSF000204; PNTB; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Complete proteome; Membrane; NAD;
KW   NADP; Oxidoreductase; Transmembrane.
FT   CHAIN         1    474       NAD(P) transhydrogenase subunit beta.
FT                                /FTId=PRO_0000199026.
FT   TRANSMEM      4     24       Potential.
FT   TRANSMEM     46     66       Potential.
FT   TRANSMEM     83    103       Potential.
FT   TRANSMEM    132    152       Potential.
FT   TRANSMEM    181    200       Potential.
FT   TRANSMEM    202    222       Potential.
FT   TRANSMEM    229    249       Potential.
FT   TRANSMEM    253    273       Potential.
FT   TRANSMEM    321    341       Potential.
FT   CONFLICT    343    343       A -> P (in Ref. 2; AAC43725).
SQ   SEQUENCE   474 AA;  50163 MW;  5F914630211FF844 CRC64;
     MSEGLVQAAY ILAALLFIMS LAGLSKHETA KAGCWFGIVG MTIALIATIF GPHSEGTFWI
     IIAMIIGGAI GVQRALKVEM TEMPELVAIL HSFVGLAAVL VGFNSYGLHH EALMPEGLDA
     AAQAAFVAEQ AVLTNIHNVE VFLGIFIGAV TFTGSVVAFG KLSGKINSKA LMLPHRHKLN
     LAALVVSALL MVAFLNNPES IFPVLLMTAI ALAFGWHLVA SIGGADMPVV VSMLNSYSGW
     AAAAAGFILN NDLLIVTGAL VGSSGAILSY IMCKAMNRSF VSVIAGGFGN DVQVSSSEEQ
     GEHRETTAEE VAELLKNASS VIITPGYGMA VAQAQYPVAD ITAKLRERGV NVRFGIHPVA
     GRLPGHMNVL LAEAKVPYDV VLEMDEINDD FADTDVVLVI GANDTVNPAA MEDPNSPIAG
     MPVLEVWKAQ NVIVFKRSMA VGYAGVQNPL FFKENTQMLF GDAKDRVEDI LKAL
//