FUNCTION: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.
CATALYTIC ACTIVITY: Nitrite + NADP⁺ + H₂O = nitrate + NADPH.
COFACTOR: Binds 1 FAD.
COFACTOR: Binds 1 heme group. The heme group is called cytochrome b-557.
COFACTOR: Binds 1 molybdenum ion.
SUBUNIT: Homodimer (By similarity).
SIMILARITY: Belongs to the nitrate reductase family.
SIMILARITY: Contains 1 cytochrome b5 heme-binding domain.
SIMILARITY: Contains 1 FAD-binding FR-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X84950; CAA59336.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S52857; S52857.

3D structure databases

HSSP

P04166; 1EUE. [HSSP ENTRY / PDB]

ModBase

P43100.

Family and domain databases

InterPro

IPR001199; Cyt_B5.
IPR001834; Cyt_B5_reductase.
IPR001709; FPN_cyt_redctse.
IPR005066; MoCF_OxRdtse_dimer.
IPR008335; Mopterin_OxRdtase_euk.
IPR012137; Nitr_rd_NADH.
IPR008333; OxRdtase_FAD-bd.
IPR001433; OxRdtase_FAD/NAD_bd.
IPR000572; OxRdtase_Mopterin-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.120.10; Cyt_B5; 1.
G3DSA:2.60.40.650; MoCF_oxrdtse_dimer; 1.
G3DSA:3.90.420.10; Oxred_molyb_bd; 1.

Pfam

PF00173; Cyt-b5; 1.
PF00970; FAD_binding_6; 1.
PF03404; Mo-co_dimer; 1.
PF00175; NAD_binding_1; 1.
PF00174; Oxidored_molyb; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000233; Nitr_rd_NADH; 1.

PRINTS

PR00406; CYTB5RDTASE.
PR00363; CYTOCHROMEB5.
PR00407; EUMOPTERIN.
PR00371; FPNCR.

ProDom

PD000612; Cyt_B5; 1.
[Domain structure / List of seq. sharing at least 1 domain]

PROSITE

PS00191; CYTOCHROME_B5_1; 1.
PS50255; CYTOCHROME_B5_2; 1.
PS51384; FAD_FR; 1.
PS00559; MOLYBDOPTERIN_EUK; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

FAD; Flavoprotein; Heme; Iron; Metal-binding; Molybdenum; NADP; Nitrate assimilation; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 894`	`894`	`Nitrate reductase [NADPH].`	`PRO_0000166041`
`DOMAIN`	`535 610`	`76`	`Cytochrome b5 heme-binding.`
`DOMAIN`	`638 749`	`112`	`FAD-binding FR-type.`
`METAL`	`169 169`		`Molybdenum-pterin (Potential).`
`METAL`	`220 220`		`Molybdenum-pterin (Potential).`
`METAL`	`570 570`		`Iron (heme axial ligand) (By similarity).`
`METAL`	`593 593`		`Iron (heme axial ligand) (By similarity).`
`DISULFID`	`418 418`		`Interchain (Potential).`

Sequence information

Length: 894 AA [This is the length of the unprocessed precursor]

Molecular weight: 99934 Da [This is the MW of the unprocessed precursor]

CRC64: D0ED234BF1B1322B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAVKSQLGVT YTTKTFPPSP PRTVGNSHAG SDDERDEVDA TPTTPPVEKL GQLLKPYSLP 

        70         80         90        100        110        120 
PTNTPTHVLP EDLKTPDHRV NRDPRLIRLT GVHPFNVEPP LTDLYDEGFL NSENLHYVRN 

       130        140        150        160        170        180 
HGPVPHCPDD ESLNWTFTVD GLVEKPFTIA VRDLIQKYDQ FTYPVTLVCA GNRRKEQNVV 

       190        200        210        220        230        240 
RKSKGFSWGA AGLSTALWTG VPIGALLRMA KPKRAAKYVC FEGADKLPNG YYGTSVKLNW 

       250        260        270        280        290        300 
CMDENRGIMV AHKMNGQSLH PDHGKPVRII IPGQIGGRSV KWLKKITITS EPSDNWYHIY 

       310        320        330        340        350        360 
DNRVLPTTIS PDASANLPDV WKDEKYAIYD LNANSAICYP RHDERLVLAT APDTYKVRGY 

       370        380        390        400        410        420 
AYGGGGKRIT RLEVTLNKGK SWLLAGIHYP EDDYRRAPDG DLLYGGSTDM WWRETCFCWC 

       430        440        450        460        470        480 
FWEIDIPVAD LSAADDIMIR AMDEGMMVQP RDMYWSVLGM MNNPWFRVVI HKEDGALRFE 

       490        500        510        520        530        540 
HPTQPALMPG GWMERVKRRG GNLTNGFWGE KTAAEEEQVL AEPEKEICMT NPKVVRIISL 

       550        560        570        580        590        600 
EELKAHEGEM EPWFVVNGHV YNGTPYLDNH PGGATSIINA AAQDATEEFM TIHSENAKAM 

       610        620        630        640        650        660 
MPQYHIGTLN DAARKALEGS AEESPASDPT RAVFLQPKYW SKAILETKTD VSSDSKIFSF 

       670        680        690        700        710        720 
RLDHAAQSIG LPTGQHLLVR LRDPATREAV IRAYTPLSET HAKGQLDILI KIYRDVPGQP 

       730        740        750        760        770        780 
GGKMTQALDS IPLGHFVDIK GPVGKFEYLG KGHCTVSGTS RHVRRFVMIC AGSGVTPIFQ 

       790        800        810        820        830        840 
VLRAVTSDAQ DGTECLVLDG NRCEKDILCR EELDAMVARA PARTTLLHKL SRPDASWCGL 

       850        860        870        880        890 
RGRMDKEYLE EHIGGFRKSD GREMVLVCGP AALEETVRSV LVEMAWKPED MLFF

P43100 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools