ID   DYR_HAEIN               Reviewed;         160 AA.
AC   P43791;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-NOV-2008, entry version 60.
DE   RecName: Full=Dihydrofolate reductase;
DE            EC=1.5.1.3;
GN   Name=folA; Synonyms=folH; OrderedLocusNames=HI0899;
OS   Haemophilus influenzae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=727;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   MEDLINE=95350630; PubMed=7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M.,
RA   Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D.,
RA   Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C.,
RA   Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M.,
RA   Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O.,
RA   Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Isolate R1042, Isolate R1047, and Isolate R906;
RA   de Groot R.;
RL   Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-
CC       dihydrofolate + NADPH.
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis;
CC       tetrahydrofolate from dihydrofolate: step 1/1.
CC   -!- MISCELLANEOUS: Isolates R906, R1042, and R1047 are trimethoprim-
CC       resistant.
CC   -!- MISCELLANEOUS: The reaction catalyzed by this enzyme represents an
CC       essential step for de novo glycine and purine synthesis, DNA
CC       precursor synthesis, and for the conversion of dUMP to dTMP.
CC   -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC   -!- SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.
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DR   EMBL; L42023; AAC22559.1; -; Genomic_DNA.
DR   EMBL; X84207; CAA58993.1; -; Genomic_DNA.
DR   EMBL; X84205; CAA58991.1; -; Genomic_DNA.
DR   EMBL; X84206; CAA58992.1; -; Genomic_DNA.
DR   PIR; C64101; C64101.
DR   PIR; S52336; S52336.
DR   PIR; S52337; S52337.
DR   PIR; S52338; S52338.
DR   RefSeq; NP_439060.1; -.
DR   HSSP; P00379; 1DHI.
DR   GeneID; 949905; -.
DR   GenomeReviews; L42023_GR; HI0899.
DR   KEGG; hin:HI0899; -.
DR   TIGR; HI0899; -.
DR   HOGENOM; P43791; -.
DR   BioCyc; HINF71421:HI_0899-MON; -.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IEA:InterPro.
DR   GO; GO:0050661; F:NADP binding; IEA:InterPro.
DR   GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR   GO; GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR012259; DHFR.
DR   InterPro; IPR001796; DHFR_reg.
DR   PANTHER; PTHR11549:SF1; DHFR; 1.
DR   Pfam; PF00186; DHFR_1; 1.
DR   PRINTS; PR00070; DHFR.
DR   PROSITE; PS00075; DHFR_1; 1.
DR   PROSITE; PS51330; DHFR_2; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Complete proteome; NADP; One-carbon metabolism;
KW   Oxidoreductase; Trimethoprim resistance.
FT   CHAIN         1    160       Dihydrofolate reductase.
FT                                /FTId=PRO_0000186391.
FT   DOMAIN        2    159       DHFR.
FT   VARIANT      13     13       N -> S (in strain: Isolate R1042 and
FT                                Isolate R1047).
FT   VARIANT      21     21       M -> I (in strain: Isolate R1047).
FT   VARIANT      54     54       P -> A (in strain: Isolate R1047).
FT   VARIANT      56     56       P -> A (in strain: Isolate R906).
FT   VARIANT      67     67       L -> P (in strain: Isolate R1042).
FT   VARIANT      69     69       E -> K (in strain: Isolate R1042).
FT   VARIANT      74     74       I -> V (in strain: Isolate R1042).
FT   VARIANT      77     77       D -> N (in strain: Isolate R1042).
FT   VARIANT      79     79       F -> L (in strain: Isolate R1042).
FT   VARIANT      95     95       I -> L (in strain: Isolate R1047).
FT   VARIANT     135    135       E -> K (in strain: Isolate R1042).
FT   VARIANT     142    142       R -> H (in strain: Isolate R1042).
FT   VARIANT     154    154       F -> S (in strain: Isolate R1042).
SQ   SEQUENCE   160 AA;  18913 MW;  384A1D64092B8C4F CRC64;
     MTFSLIVATT LNNVIGKDNQ MPWHLPADLA WFRQNTTGKP VIMGRKTFES IGRPLPKRTN
     IVLSRQLFEH EGVIWKDSFE SAVNFVRDFD EIMLIGGGEL FKQYLPKADK LYLTQIQTEL
     DGDTFFPQLN WEEWEIEFDE YRKADEQNRY DCRFLILTRK
//