[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). DOI=10.1101/gad.1255404; PubMed=15574588 [NCBI, ExPASy, EBI, Israel, Japan] Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R.; "The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans."; Genes Dev. 18:3004-3009(2004).
[2]	ERRATUM. Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R.; Genes Dev. 19:188-188(2005).
[3]	ERRATUM. Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R.; Genes Dev. 19:411-411(2005).
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=Bristol N2; DOI=10.1038/368032a0; PubMed=7906398 [NCBI, ExPASy, EBI, Israel, Japan] Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P.; "2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans."; Nature 368:32-38(1994).
[5]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING. STRAIN=Bristol N2; DOI=10.1126/science.282.5396.2012; PubMed=9851916 [NCBI, ExPASy, EBI, Israel, Japan] The C. elegans sequencing consortium; "Genome sequence of the nematode C. elegans: a platform for investigating biology."; Science 282:2012-2018(1998).

Comments

FUNCTION: Probably does not act as a sensor that couples lifespan to information about energy levels and insulin-like signals.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	a
Isoform ID	P45894-1
This is the isoform sequence displayed in this entry.

Name	b
Isoform ID	P45894-2
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_017190.

SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
SIMILARITY: Contains 1 protein kinase domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY347272; AAR06927.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U00025; ABA54413.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U00025; ABA54414.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

S44859; S44859.

NP_741254.3; -.

3D structure databases

HSSP

Q63450; 1A06. [HSSP ENTRY / PDB]

ModBase

P45894.

Organism-specific databases

WormBase

WBGene00019801; aak-1.

WormPep

PAR2.3a; CE39167. [WormPep / WorfDB]
PAR2.3b; CE39168. [WormPep / WorfDB]

Gene expression databases

ArrayExpress

P45894; -.

Ontologies

GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0004674;	Molecular function: protein serine/threonine kinase activity (inferred from electronic annotation from InterPro).
GO:0006468;	Biological process: protein amino acid phosphorylation (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR015741; AMPK.
IPR000719; Prot_kinase_core.
IPR017441; Protein_kinase_ATP_bd_CS.
IPR017442; Se/Thr_pkinase-rel.
IPR008271; Ser_thr_pkin_AS.
IPR002290; Ser_thr_pkinase.
Graphical view of domain structure.

PANTHER

PTHR22982:SF61; AMPK; 1.

Pfam

PF00069; Pkinase; 1.
Pfam graphical view of domain structure.

ProDom

PD000001; Prot_kinase; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00220; S_TKc; 1.
SMART graphical view of domain structure.

PROSITE

PS00107; PROTEIN_KINASE_ATP; 1.
PS50011; PROTEIN_KINASE_DOM; 1.
PS00108; PROTEIN_KINASE_ST; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P45894.

Genome annotation databases

Ensembl

PAR2.3; Caenorhabditis elegans. [Contig view]

GeneID

176230; -.

KEGG

cel:PAR2.3; -.

Other

NextBio

891672; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Serine/threonine-protein kinase; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 589`	`589`	`5'-AMP-activated protein kinase catalytic subunit alpha-1.`	`PRO_0000085598`
`DOMAIN`	`24 276`	`253`	`Protein kinase.`
`NP_BIND`	`30 38`	`9`	`ATP (By similarity).`
`ACT_SITE`	`147 147`		`Proton acceptor (By similarity).`
`BINDING`	`53 53`		`ATP (By similarity).`
`VAR_SEQ`	`1 171`		`Missing (in isoform b).`	`VSP_017190`
`CONFLICT`	`205 205`		`L -> P (in Ref. 1; AAR06927).`
`CONFLICT`	`277 277`		`R -> H (in Ref. 1; AAR06927).`

Sequence information

Length: 589 AA [This is the length of the unprocessed precursor]

Molecular weight: 65620 Da [This is the MW of the unprocessed precursor]

CRC64: AED4E43EFBFDD863 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPPSGRFDRT IALAGTGHLK IGNFVIKETI GKGAFGAVKR GTHIQTGYDV AIKILNRGRM 

        70         80         90        100        110        120 
KGLGTVNKTR NEIDNLQKLT HPHITRLFRV ISTPSDIFLV MELVSGGELF SYITRKGALP 

       130        140        150        160        170        180 
IRESRRYFQQ IISGVSYCHN HMIVHRDLKP ENLLLDANKN IKIADFGLSN YMTDGDLLST 

       190        200        210        220        230        240 
ACGSPNYAAP ELISNKLYVG PEVDLWSCGV ILYAMLCGTL PFDDQNVPTL FAKIKSGRYT 

       250        260        270        280        290        300 
VPYSMEKQAA DLISTMLQVD PVKRADVKRI VNHSWFRIDL PYYLFPECEN ESSIVDIDVV 

       310        320        330        340        350        360 
QSVAEKFDVK EEDVTGALLA EDHHHFLCIA YRLEVNHKRN ADESSQKAME DFWEIGKTMK 

       370        380        390        400        410        420 
MGSTSLPVGA TTKTNVGRKI LEGLKKEQKK LTWNLGIRAC LDPVETMKHV FLSLKSVDME 

       430        440        450        460        470        480 
WKVLSMYHII VRSKPTPINP DPVKVSLQLF ALDKKENNKG YLLDFKGLTE DEEAVPPSRC 

       490        500        510        520        530        540 
RSRAASVSVT LAKSKSDLNG NSSKVPMSPL SPMSPISPSV NIPKVRVDDA DASLKSSLNS 

       550        560        570        580 
SIYMADIENS MESLDEVSTQ SSEPEAPIRS QTMEFFATCH IIMQALLAE

P45894 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools