NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=RIMD 2210633 / Serotype O3:K6;
DOI=10.1016/S0140-6736(03)12659-1; PubMed=12620739 [NCBI, ExPASy, EBI, Israel, Japan]
Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
"Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae.";
Lancet 361:743-749(2003).

[2]

NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 6-138.
STRAIN=BB22;
PubMed=8021208 [NCBI, ExPASy, EBI, Israel, Japan]
McCarter L.L.;
"MotY, a component of the sodium-type flagellar motor.";
J. Bacteriol. 176:4219-4225(1994).

Comments

FUNCTION: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione (By similarity).
CATALYTIC ACTIVITY: (R)-S-lactoylglutathione = glutathione + methylglyoxal.
COFACTOR: Binds 1 nickel ion per subunit (By similarity).
PATHWAY: Secondary metabolite metabolism; methylglyoxal degradation; D-lactate from methylglyoxal: step 1/2 .
SIMILARITY: Belongs to the glyoxalase I family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000031; BAC60372.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U06949; AAA21576.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_798488.1; -.

3D structure databases

HSSP

Q59384; 1F9Z. [HSSP ENTRY / PDB]

SMR

P46235; 5-128.

ModBase

P46235.

Enzyme and pathway databases

BioCyc

VPAR223926:VP2109-MON; -.

Ontologies

GO:0004462;	Molecular function: lactoylglutathione lyase activity (inferred from electronic annotation from InterPro).
GO:0016151;	Molecular function: nickel ion binding (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR004360; Glyas_bleo-R_dOase.
IPR004361; Glyoxalase_1.
Graphical view of domain structure.

Pfam

PF00903; Glyoxalase; 1.
Pfam graphical view of domain structure.

ProDom

PD002334; Gly_diox; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR00068; glyox_I; 1.

PROSITE

PS00934; GLYOXALASE_I_1; 1.
PS00935; GLYOXALASE_I_2; 1.

ProtoNet

P46235.

Genome annotation databases

1189621; -.

BA000031_GR; VP2109.

vpa:VP2109; -.

fig|223926.1.peg.2109; -.

Phylogenomic databases

HOGENOM

P46235; -.

Genome annotation databases

CMR

P46235; VP2109.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Lyase; Metal-binding; Nickel.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 138`	`138`	`Probable lactoylglutathione lyase.`	`PRO_0000168098`
`METAL`	`8 8`		`Nickel (By similarity).`
`METAL`	`59 59`		`Nickel (By similarity).`
`METAL`	`77 77`		`Nickel (By similarity).`
`METAL`	`125 125`		`Nickel (By similarity).`

Sequence information

Length: 138 AA [This is the length of the unprocessed precursor]

Molecular weight: 15034 Da [This is the MW of the unprocessed precursor]

CRC64: 5738D502E3CA35FC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSNGRILHTM LRVGDLDKSI KFYTEVMGMQ LLRTNENKEY EYTLAFVGYG DESQGAVIEL 

        70         80         90        100        110        120 
TYNWGKTEYD LGTAFGHIAI GVDDIYATCD AIKAAGGNVT REAGPVKGGT THIAFVKDPD 

       130 
GYMIELIQNK QASAGLEG

P46235 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools