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UniProtKB/Swiss-Prot entry P48534

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name APX1_PEA
Primary accession number P48534
Secondary accession numbers None
Integrated into Swiss-Prot on February 1, 1996
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    September 23, 2008 (Entry version 66)
Name and origin of the protein
Protein name L-ascorbate peroxidase, cytosolic
Synonyms AP
EC 1.11.1.11
PsAPx01
Gene name
Name: APX1
Synonyms: APPX1
From
Pisum sativum (Garden pea) [TaxID: 3888] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Fabeae; Pisum.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=cv. Little Marvel;
TISSUE=Leaf;
DOI=10.1016/0014-5793(91)81083-K; PubMed=1915856 [NCBI, ExPASy, EBI, Israel, Japan]
Mittler R., Zilinskas B.A.;
"Molecular cloning and nucleotide sequence analysis of a cDNA encoding pea cytosolic ascorbate peroxidase.";
FEBS Lett. 289:257-259(1991).
[2]
 NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Little Marvel;
PubMed=1400489 [NCBI, ExPASy, EBI, Israel, Japan]
Mittler R., Zilinskas B.A.;
"Molecular cloning and characterization of a gene encoding pea cytosolic ascorbate peroxidase.";
J. Biol. Chem. 267:21802-21807(1992).
[3]
 ERRATUM.
Mittler R., Zilinskas B.A.;
J. Biol. Chem. 268:4568-4568(1993).
[4]
 X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
DOI=10.1021/bi00013a023; PubMed=7703247 [NCBI, ExPASy, EBI, Israel, Japan]
Patterson W.R., Poulos T.L.;
"Crystal structure of recombinant pea cytosolic ascorbate peroxidase.";
Biochemistry 34:4331-4341(1995).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M93051; AAA33645.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X62077; CAA43992.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A45116; A45116.
3D structure databases
PDB
1APX; X-ray; 2.20 A; A/B/C/D=1-250.[ExPASy / RCSB / EBI]
PDBsum 1APX; -.
ModBase P48534.
Protein family/group databases
PeroxiBase 2462; PsAPx01.
Family and domain databases
InterPro IPR002207; Asc_perxdse.
IPR002016; Haem_peroxidase_pln/fun/bac.
Graphical view of domain structure.
Pfam PF00141; peroxidase; 1.
Pfam graphical view of domain structure.
PRINTS PR00459; ASPEROXIDASE.
PR00458; PEROXIDASE.
PROSITE PS00435; PEROXIDASE_1; 1.
PS00436; PEROXIDASE_2; 1.
PS50873; PEROXIDASE_4; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P48534.
Other
LinkHub P48534; -.
ProtoNet P48534.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Calcium; Cytoplasm; Heme; Hydrogen peroxide; Iron; Metal-binding; Oxidoreductase; Peroxidase; Potassium; Stress response.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   250  249     L-ascorbate peroxidase, cytosolic. PRO_0000055597
ACT_SITE   42    42        Proton acceptor. 
METAL   163   163        Iron (heme axial ligand). 
METAL   164   164        Potassium or calcium. 
METAL   180   180        Potassium or calcium. 
METAL   182   182        Potassium or calcium. 
METAL   185   185        Potassium or calcium; via carbonyl oxygen. 
METAL   187   187        Potassium or calcium. 
SITE   38    38  1     Transition state stabilizer. 
HELIX   10    30  21      
HELIX   33    44  12      
TURN   49    52  4      
STRAND   55    58  4      
HELIX   59    61  3      
HELIX   63    66  4      
HELIX   69    71  3      
HELIX   74    86  13      
HELIX   93   107  15      
HELIX   138   145  8      
TURN   146   148  3      
HELIX   153   160  8      
HELIX   161   164  4      
TURN   170   172  3      
STRAND   177   181  5      
HELIX   189   195  7      
HELIX   206   209  4      
TURN   210   212  3      
HELIX   217   226  10      
HELIX   228   243  16      
TURN   244   246  3      
Sequence information
Length: 250 AA [This is the length of the unprocessed precursor] Molecular weight: 27193 Da [This is the MW of the unprocessed precursor] CRC64: 6F51006D0A13B42C [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MGKSYPTVSP DYQKAIEKAK RKLRGFIAEK KCAPLILRLA WHSAGTFDSK TKTGGPFGTI 

        70         80         90        100        110        120 
KHQAELAHGA NNGLDIAVRL LEPIKEQFPI VSYADFYQLA GVVAVEITGG PEVPFHPGRE 

       130        140        150        160        170        180 
DKPEPPPEGR LPDATKGSDH LRDVFGKAMG LSDQDIVALS GGHTIGAAHK ERSGFEGPWT 

       190        200        210        220        230        240 
SNPLIFDNSY FTELLTGEKD GLLQLPSDKA LLTDSVFRPL VEKYAADEDV FFADYAEAHL 

       250 
KLSELGFAEA
P48534 in FASTA format

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