NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ML2;
PubMed=8824602 [NCBI, ExPASy, EBI, Israel, Japan]
Fong K.P.Y., Goh C.B.H., Tan H.M.;
"Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase.";
J. Bacteriol. 178:5592-5601(1996).

Comments

FUNCTION: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions (By similarity).
CATALYTIC ACTIVITY: Glycerol + NAD⁺ = glycerone + NADH.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
PATHWAY: Polyol metabolism; glycerol fermentation; glycerone phosphate from glycerol (oxidative route): step 1/2 .
SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF148496; AAC44426.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

Q9WYQ4; 1KQ3. [HSSP ENTRY / PDB]

ModBase

P50173.

Family and domain databases

InterPro

IPR001670; Fe_AlcDHase.
IPR016205; Glycerol_DH.
Graphical view of domain structure.

Pfam

PF00465; Fe-ADH; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000112; Glycerol_dehydrogenase; 1.

PROSITE

PS00913; ADH_IRON_1; 1.
PS00060; ADH_IRON_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Glycerol metabolism; Metal-binding; NAD; Oxidoreductase; Plasmid; Zinc.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 365`	`365`	`Glycerol dehydrogenase.`	`PRO_0000087830`
`NP_BIND`	`94 98`	`5`	`NAD (By similarity).`
`NP_BIND`	`116 119`	`4`	`NAD (By similarity).`
`METAL`	`171 171`		`Zinc; catalytic (By similarity).`
`METAL`	`254 254`		`Zinc; catalytic (By similarity).`
`METAL`	`271 271`		`Zinc; catalytic (By similarity).`
`BINDING`	`37 37`		`NAD (By similarity).`
`BINDING`	`121 121`		`Substrate (By similarity).`
`BINDING`	`125 125`		`NAD (By similarity).`
`BINDING`	`127 127`		`NAD; via carbonyl oxygen (By similarity).`
`BINDING`	`131 131`		`NAD (By similarity).`
`BINDING`	`171 171`		`Substrate (By similarity).`
`BINDING`	`254 254`		`Substrate (By similarity).`
`BINDING`	`271 271`		`Substrate (By similarity).`

Sequence information

Length: 365 AA [This is the length of the unprocessed precursor]

Molecular weight: 38316 Da [This is the MW of the unprocessed precursor]

CRC64: D6AEAA16D52A8D47 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDRAIQSPGK YVQGADALQR LGDYLKPLAD SWLVIADKFV LGFAEDTIRQ SLSKAGLAMD 

        70         80         90        100        110        120 
IVAFNGECSQ GEVDRLCQLA TQNGRSAIVG IGGGKTLDTA KAVAFFQKVP VAVAPTIAST 

       130        140        150        160        170        180 
DAPCSALSVL YTDEGEFDRY LMLPTNPALV VVDTAIVARA PARLLAAGIG DALATWFEAR 

       190        200        210        220        230        240 
AASRSSAATM AGGPATQTAL NLARFCYDTL LEEGEKAMLA VQAQVVTPAL ERIVEANTYL 

       250        260        270        280        290        300 
SGVGFESGGV AAAHAVHNGL TAVAETHHFY HGEKVAFGVL VQLALENASN AEMQEVMSLC 

       310        320        330        340        350        360 
HAVGLPITLA QLDITEDIPT KMRAVAELAC APGETIHNMP GGVTVEQVYG ALLVADQLGQ 


HFLEF

P50173 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools