NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 3-19, AND CHARACTERIZATION.
STRAIN=ATCC 19357 / DSM 3503 / IFO 14819 / LMG 1408 / NCIB 9013;
PubMed=7751271 [NCBI, ExPASy, EBI, Israel, Japan]
Klasen R., Bringer-Meyer S., Sahm H.;
"Biochemical characterization and sequence analysis of the gluconate:NADP 5-oxidoreductase gene from Gluconobacter oxydans.";
J. Bacteriol. 177:2637-2643(1995).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=621H;
DOI=10.1038/nbt1062; PubMed=15665824 [NCBI, ExPASy, EBI, Israel, Japan]
Prust C., Hoffmeister M., Liesegang H., Wiezer A., Fricke W.F., Ehrenreich A., Gottschalk G., Deppenmeier U.;
"Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans.";
Nat. Biotechnol. 23:195-200(2005).

Comments

FUNCTION: Involved in the non-phosphorylative, ketogenic oxidation of glucose and oxidizes gluconate to 5-ketogluconate. Dependent on NADP, almost inactive with NAD.
CATALYTIC ACTIVITY: D-gluconate + NAD(P)⁺ = 5-dehydro-D-gluconate + NAD(P)H.
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X80019; CAA56322.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
CP000009; AAW61923.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A57149; A57149.

RefSeq

YP_192579.1; -.

3D structure databases

HSSP

P08074; 1CYD. [HSSP ENTRY / PDB]

ModBase

P50199.

Enzyme and pathway databases

BioCyc

GOXY290633:GOX2187-MON; -.

Family and domain databases

InterPro

IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
Graphical view of domain structure.

PANTHER

PTHR19410; ADH_short_C2; 1.

Pfam

PF00106; adh_short; 1.
Pfam graphical view of domain structure.

PRINTS

PR00081; GDHRDH.
PR00080; SDRFAMILY.

PROSITE

PS00061; ADH_SHORT; 1.

BLOCKS

P50199.

Genome annotation databases

GeneID

3250506; -.

GenomeReviews

CP000009_GR; GOX2187.

KEGG

gox:GOX2187; -.

NMPDR

fig|290633.1.peg.2124; -.

Phylogenomic databases

HOGENOM

P50199; -.

Genome annotation databases

CMR

P50199; GOX2187.

Other

ProtoNet

P50199.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; Direct protein sequencing; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 256`	`256`	`Gluconate 5-dehydrogenase.`	`PRO_0000054701`
`NP_BIND`	`15 39`	`25`	`NADP (By similarity).`
`ACT_SITE`	`160 160`		`Proton acceptor (By similarity).`
`BINDING`	`147 147`		`Substrate (By similarity).`

Sequence information

Length: 256 AA [This is the length of the unprocessed precursor]

Molecular weight: 27256 Da [This is the MW of the unprocessed precursor]

CRC64: 38B03C0399C0A07A [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSHPDLFSLS GARALVTGAS RGIGLTLAKG LARYGAEVVL NGRNAESLDS AQSGFEAEGL 

        70         80         90        100        110        120 
KASTAVFDVT DQDAVIDGVA AIERDMGPID ILINNAGIQR RAPLEEFSRK DWDDLMSTNV 

       130        140        150        160        170        180 
NAVFFVGQAV ARHMIPRGRG KIVNICSVQS ELARPGIAPY TATKGAVKNL TKGMATDWGR 

       190        200        210        220        230        240 
HGLQINGLAP GYFATEMTER LVADEEFTDW LCKRTPAGRW GQVEELVGAA VFLSSRASSF 

       250 
VNGQVLMVDG GITVSL

P50199 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools