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UniProtKB/Swiss-Prot entry P50859


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CP51_CANGA
Primary accession number P50859
Secondary accession number Q02312
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on October 1, 1996 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 53)
Name and origin of the protein
Protein name Cytochrome P450 51
Synonyms EC 1.14.13.70
CYPLI
P450-LIA1
Sterol 14-alpha-demethylase
Lanosterol 14-alpha demethylase
P450-14DM
Gene name
Name: ERG11
Synonyms: CYP51
OrderedLocusNames: CAGL0E04334g
From
Candida glabrata (Yeast) (Torulopsis glabrata) [TaxID: 5478] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; mitosporic Saccharomycetales; Candida.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=2001-L5;
PubMed=8593007 [NCBI, ExPASy, EBI, Israel, Japan]
Geber A., Hitchcock C.A., Swartz J.E., Pullen F.S., Marsden K.E., Kwon-Chung K.J., Bennett J.E.;
"Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility.";
Antimicrob. Agents Chemother. 39:2708-2717(1995).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 2001 / CBS 138 / IFO 0622 / NRRL Y-65;
DOI=10.1038/nature02579; PubMed=15229592 [NCBI, ExPASy, EBI, Israel, Japan]
Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S., Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.;
"Genome evolution in yeasts.";
Nature 430:35-44(2004).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 60-473.
STRAIN=ATCC 2001 / CBS 138 / IFO 0622 / NRRL Y-65;
PubMed=7989540 [NCBI, ExPASy, EBI, Israel, Japan]
Burgener-Kairuz P., Zuber J.P., Jaunin P., Buchman T.G., Bille J., Rossier M.;
"Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment.";
J. Clin. Microbiol. 32:1902-1907(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L40389; AAB02329.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CR380951; CAG58795.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S75389; AAB32679.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq XP_445876.1; -.
3D structure databases
ModBase P50859.
Ontologies
GO
GO:0008398; Molecular function: sterol 14-demethylase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR001128; Cyt_P450.
Graphical view of domain structure.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PROSITE PS00086; CYTOCHROME_P450; 1.
BLOCKS P50859.
Genome annotation databases
GeneID 2887532; -.
KEGG cgr:CAGL0E04334g; -.
Phylogenomic databases
HOGENOM P50859; -.
Other
DrugBank DB00639; Butoconazole.
DB01167; Itraconazole.
DB01110; Miconazole.
ProtoNet P50859.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Heme; Iron; Lipid synthesis; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   533  533     Cytochrome P450 51. PRO_0000052004
METAL   472   472        Iron (heme axial ligand) (By similarity). 
CONFLICT   64    64        I -> M (in Ref. 3; AAB32679). 
CONFLICT   473   473        I -> T (in Ref. 3; AAB32679). 
Sequence information
Length: 533 AA [This is the length of the unprocessed precursor] Molecular weight: 61305 Da [This is the MW of the unprocessed precursor] CRC64: A0506C17507E6EF7 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSTENTSLVV ELLEYVKLGL SYFQALPLAQ RVSIMVALPF VYTITWQLLY SLRKDRPPLV 

        70         80         90        100        110        120 
FYWIPWVGSA IPYGTKPYEF FEDCQKKYGD IFSFMLLGRI MTVYLGPKGH EFIFNAKLAD 

       130        140        150        160        170        180 
VSAEAAYSHL TTPVFGKGVI YDCPNHRLME QKKFVKGALT KEAFVRYVPL IAEEIYKYFR 

       190        200        210        220        230        240 
NSKNFKINEN NSGIVDVMVS QPEMTIFTAS RSLLGKEMRD KLDTDFAYLY SDLDKGFTPI 

       250        260        270        280        290        300 
NFVFPNLPLE HYRKRDHAQQ AISGTYMSLI KERREKNDIQ NRDLIDELMK NSTYKDGTKM 

       310        320        330        340        350        360 
TDQEIANLLI GVLMGGQHTS AATSAWCLLH LAERPDVQEE LYQEQMRVLN NDTKELTYDD 

       370        380        390        400        410        420 
LQNMPLLNQM IKETLRLHHP LHSLFRKVMR DVAIPNTSYV VPRDYHVLVS PGYTHLQEEF 

       430        440        450        460        470        480 
FPKPNEFNIH RWDGDAASSS AAGGDEVDYG FGAISKGVSS PYLPFGGGRH RCIGELFAYC 

       490        500        510        520        530 
QLGVLMSIFI RTMKWRYPTE GETVPPSDFT SMVTLPTAPA KIYWEKRHPE QKY 

P50859 in FASTA format

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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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