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UniProtKB/Swiss-Prot entry P52032


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GPX1_ARATH
Primary accession number P52032
Secondary accession numbers O19985 O81717
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on March 27, 2002 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 72)
Name and origin of the protein
Protein name Phospholipid hydroperoxide glutathione peroxidase 1, chloroplastic [Precursor]
Synonyms PHGPx
EC 1.11.1.12
Gene name
Name: GPX1
OrderedLocusNames: At2g25080
ORFNames: F13D4.40
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Columbia;
Gachotte D., Benveniste P.;
"Cloning and sequencing of a glutathione peroxidase homologue from Arabidopsis thaliana.";
(er) Plant Gene Register PGR95-133.
[2]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Columbia;
PubMed=9680987 [NCBI, ExPASy, EBI, Israel, Japan]
Mullineaux P.M., Karpinski S., Jimenez A., Cleary S.P., Robinson C., Creissen G.P.;
"Identification of cDNAS encoding plastid-targeted glutathione peroxidase.";
Plant J. 13:375-379(1998).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/45471; PubMed=10617197 [NCBI, ExPASy, EBI, Israel, Japan]
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
Nature 402:761-768(1999).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[5]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M300030-MCP200; PubMed=12766230 [NCBI, ExPASy, EBI, Israel, Japan]
Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.;
"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana.";
Mol. Cell. Proteomics 2:325-345(2003).
[6]
GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY, AND INDUCTION.
DOI=10.1046/j.1365-313X.2003.01901.x; PubMed=14617062 [NCBI, ExPASy, EBI, Israel, Japan]
Rodriguez Milla M.A., Maurer A., Rodriguez Huete A., Gustafson J.P.;
"Glutathione peroxidase genes in Arabidopsis are ubiquitous and regulated by abiotic stresses through diverse signaling pathways.";
Plant J. 36:602-615(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X89866; CAA61965.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ000469; CAA04112.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY035153; AAK59657.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY063024; AAL34198.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A84644; A84644.
S71250; S71250.
RefSeq NP_180080.1; -.
UniGene At.24670
3D structure databases
HSSP P00435; 1GP1. [HSSP ENTRY / PDB]
ModBase P52032.
Protein family/group databases
PeroxiBase 2499; AtGPx01.
Organism-specific databases
GeneFarm 2047; 163.
TAIR At2g25080; -.
Gene expression databases
ArrayExpress P52032; -.
GermOnline AT2G25080; Arabidopsis thaliana.
Family and domain databases
InterPro IPR000889; Glut_peroxidase.
IPR012335; Thioredoxin_fold.
Graphical view of domain structure.
Gene3D G3DSA:3.40.30.10; Thioredoxin_fold; 1.
PANTHER PTHR11592; Glut_peroxidase; 1.
Pfam PF00255; GSHPx; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000303; Glutathion_perox; 1.
PRINTS PR01011; GLUTPROXDASE.
PROSITE PS00460; GLUTATHIONE_PEROXID_1; 1.
PS00763; GLUTATHIONE_PEROXID_2; 1.
PS51355; GLUTATHIONE_PEROXID_3; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P52032.
Genome annotation databases
GeneID 817046; -.
GenomeReviews CT485783_GR; AT2G25080.
KEGG ath:AT2G25080; -.
NMPDR fig|3702.1.peg.9525; -.
Other
ProtoNet P52032.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Chloroplast; Complete proteome; Oxidoreductase; Peroxidase; Plastid; Stress response; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    64  64     Chloroplast (Potential). 
CHAIN   65   236  172     Phospholipid hydroperoxide glutathione peroxidase 1, chloroplastic. PRO_0000013086
ACT_SITE   111   111        By similarity. 
CONFLICT   18    18        V -> F (in Ref. 1; CAA61965). 
CONFLICT   236   236        A -> ELKNF (in Ref. 1). 
Sequence information
Length: 236 AA [This is the length of the unprocessed precursor] Molecular weight: 26016 Da [This is the MW of the unprocessed precursor] CRC64: D676C0381526C37A [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVSMTTSSSS YGTFSTVVNS SRPNSSATFL VPSLKFSTGI SNFANLSNGF SLKSPINPGF 

        70         80         90        100        110        120 
LFKSRPFTVQ ARAAAEKTVH DFTVKDIDGK DVALNKFKGK VMLIVNVASR CGLTSSNYSE 

       130        140        150        160        170        180 
LSHLYEKYKT QGFEILAFPC NQFGFQEPGS NSEIKQFACT RFKAEFPIFD KVDVNGPSTA 

       190        200        210        220        230 
PIYEFLKSNA GGFLGGLIKW NFEKFLIDKK GKVVERYPPT TSPFQIEKDI QKLLAA 

P52032 in FASTA format

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View entry in raw text format (no links)
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