NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-18.
DOI=10.1074/jbc.270.6.2827; PubMed=7852356 [NCBI, ExPASy, EBI, Israel, Japan]
Mukhopadhyay B., Purwantini E., Pihl T.D., Reeve J.N., Daniels L.;
"Cloning, sequencing, and transcriptional analysis of the coenzyme F420-dependent methylene-5,6,7,8-tetrahydromethanopterin dehydrogenase gene from Methanobacterium thermoautotrophicum strain Marburg and functional expression in Escherichia coli.";
J. Biol. Chem. 270:2827-2832(1995).

Comments

FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT.
CATALYTIC ACTIVITY: 5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420.
ENZYME REGULATION: Oxygen-stable, strictly dependent on coenzyme F420, thermostable, thermophilic and exhibits maximum activity at pH 4 and 55-65 degrees Celsius.
PATHWAY: One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methylene-H(4)MPT from 5,10-methenyl-H(4)MPT (cofactor F420 route): step 1/1 .
SUBUNIT: Found to be tightly associated with methyl-coenzyme M methylreductase.
SIMILARITY: Belongs to the MTD family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L37108; AAC41463.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

P55300.

Ontologies

GO:0030268;	Molecular function: methylenetetrahydromethanopterin dehydrogenase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00058; -; 1.
PBIL [Tree]

InterPro

IPR002844; Methylene_DHase.
Graphical view of domain structure.

Pfam

PF01993; MTD; 1.
Pfam graphical view of domain structure.

PIRSF005627; MTD; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Methanogenesis; One-carbon metabolism; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 276`	`275`	`F420-dependent methylenetetrahydromethanopterin dehydrogenase.`	`PRO_0000075042`

Sequence information

Length: 276 AA [This is the length of the unprocessed precursor]

Molecular weight: 29614 Da [This is the MW of the unprocessed precursor]

CRC64: E32142964BF3BAC3 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVVKIGIIKC GNIGTSPVLD LLLDERADRP NIDVCVVGSG AKMNPDEIER AVPTMLEMER 

        70         80         90        100        110        120 
DFVIFISPNP GAPGPAKARE LLSAADVPAM IIGDAPGLRV KDEIEEQGLG YIIVKADPMI 

       130        140        150        160        170        180 
GARREFLDPT EMASFNSDVI KVLAFTGAYR VVQNTIDAMI ADVEAGKAPE LPQVVIDTDK 

       190        200        210        220        230        240 
AVEAAGYTNP YAKAKAMAAY EIATKVADID VRGCFMVQDP DQYIPIVASA HEMLSAAAKL 

       250        260        270 
AIEAREIEKA NDTVLRTPHG KEGKTLSKKD LLAKPE

P55300 in FASTA format

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View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools